Probing the interaction of human serum albumin with DPPH in the absence and presence of the eight antioxidants

Li, Xiangrong; Chen, Dejun; Wang, Gongke; Lü, Yan

doi:10.1016/j.saa.2014.08.140

Cited by 13 publications

(3 citation statements)

References 32 publications

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“…The binding of HSA by various ligands has an influence on the course of the reaction between DPPH and HSA. This phenomenon is especially noticeable, when ligands such as ascorbic acid, α-tocopherol, melatonin or β-carotene are strong antioxidants [ 8 ]. Cao et al studied the incubation of various polyphenols with bovine serum albumin (BSA), and they observed that the 7-day incubation caused a higher antioxidant potential in polyphenols relative to DPPH than that in samples without BSA [ 7 ].…”

Section: Resultsmentioning

confidence: 99%

“…Mutual interactions between various substances such as drugs can have a significant impact on their biological activity [ 6 ]. Chemicals bound with HSA modulate its antioxidant activity as well as modify HSA properties and structure [ 7 , 8 ]. The interaction between biologically active substances are antagonistic, synergistic and additive.…”

Section: Introductionmentioning

confidence: 99%

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Comparison of Losartan and Furosemide Interaction with HSA and Their Influence on HSA Antioxidant Potential

et al. 2022

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Serum albumin (HSA) is the most important protein in human body. Due to the antioxidant activity, HSA influences homeostasis maintenance and transport of drugs as well as other substances. It is noteworthy that ligands, such as popular drugs, modulate the antioxidant activity of HSA. The aim of this study was to analyze the influence of losartan (LOS) and furosemide (FUR) on HSA antioxidant properties as well as the interaction between these drugs and protein using calorimetric and spectroscopic methods. LOS and FUR showed the high affinity for human serum albumin, and the binding reactions between them were spontaneous and exothermic. LOS and FUR, separately and together in the system, have no significant impact on the secondary HSA structure; however they have significant impact on the tertiary HSA structure. LOS and FUR mixed with HSA have the ability to scavenge free radicals, and the ligand(s)–HSA interactions were synergistic.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Comparison of Losartan and Furosemide Interaction with HSA and Their Influence on HSA Antioxidant Potential

et al. 2022

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“…Newly synthesized compounds (2a and 2b) were subjected to 2,2'‐diphenyl‐1‐picryl hydrazyl (DPPH) radical scavenging assay in order to estimate their antioxidant properties. Among the different antioxidant assays, DPPH analysis has been most widely used technique for estimating the free radical scavenging activity of antioxidants . The scavenging efficiency of test compounds against DPPH radicals was resolved spectrophotometrically and the findings are given in Table .…”

Section: Resultsmentioning

confidence: 99%

New phthalimide‐appended Schiff bases: Studies of DNA binding, molecular docking and antioxidant activities

et al. 2016

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Herein, we investigated new phthalimide-based Schiff base molecules as promising DNA-binding and free radical scavenging agents. Physicochemical properties of these molecules were demonstrated on the basis of elemental analysis, ultraviolet-visible (UV-Vis), infra-red (IR), H and C nuclear magnetic resonance (NMR) spectroscopy. All spectral data are agreed well with the proposed Schiff base framework. The DNA-binding potential of synthesized compounds were investigated by means of UV-visible, fluorescence, iodide quenching, circular dichroism, viscosity and thermal denaturation studies. The intrinsic binding constants (K ) were calculated from absorption studies were found to be 1.1 × 10 and 1.0 × 10 M for compounds 2a and 2b suggesting that compound 2a binding abilities with DNA were stronger than the compound 2b. Our studies showed that the presented compounds interact with DNA through groove binding. Molecular docking studies were carried out to predict the binding between Ct-DNA and test compounds. Interestingly, in silico predictions were corroborated with in vitro DNA-binding conclusions. Furthermore, the title compounds displayed remarkable antioxidant activity compared with reference standard.

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Analysis of binding properties and interaction of thiabendazole and its metabolite with human serum albumin via multiple spectroscopic methods

Sun

Yang

et al. 2017

Food Chemistry

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Probing the interaction of human serum albumin with DPPH in the absence and presence of the eight antioxidants

Cited by 13 publications

References 32 publications

Comparison of Losartan and Furosemide Interaction with HSA and Their Influence on HSA Antioxidant Potential

Comparison of Losartan and Furosemide Interaction with HSA and Their Influence on HSA Antioxidant Potential

New phthalimide‐appended Schiff bases: Studies of DNA binding, molecular docking and antioxidant activities

Analysis of binding properties and interaction of thiabendazole and its metabolite with human serum albumin via multiple spectroscopic methods

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