Probing the interaction between 3 flavonoids and pancreatic lipase by methods of fluorescence spectroscopy and enzymatic kinetics

Li, Yanqin; Yang, Peng; Gao, Fei; ZongWen, Zhang; Wu, Bin

doi:10.1007/s00217-011-1491-z

Cited by 56 publications

(59 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The 7 inhibition lines of PL intersect on the x-axis indicated that the mechanism of pancreatic lipase inhibition by WE, ME, EE, CE, NBE and EAE was noncompetitive type. This was similar to the result obtain by Li [3] but diametrically opposed to Martins [18]. The data showed that the extracts and substrate could bind to pancreatic lipase simultaneously.…”

Section: Kinetics Of Enzyme Inhibitionsupporting

confidence: 89%

“…When the concentration of orlistat > 400 μg/mL, the inhibition rate reaches 100%. Nevertheless, the extracts still showed a strong inhibitory activity of pancreatic lipase compared with quercetin, isoquercitrin and rutin [3], especially ME. To some extent, The IC 50 values showed the inhibitory effect of inhibitors on enzyme active under a particular substrate concentration.…”

Section: Pancreatic Lipase Inhibitory Activitymentioning

confidence: 88%

“…Obesity not only affects human appearance, but also causes many diseases, such as hepatic adipose infiltration [1], arteriosclerosis [2], hypertension, coronary heart disease, type II diabetes and other chronic diseases [3]. Due to the increasing obesity rates, the World Health Organization has prompted to promote strategies to prevent and control its progress [4].…”

Section: Introductionmentioning

confidence: 99%

“…Dietary triglycerides are hydrolyzed by lipase to monoglycerides, fatty acids and other small molecules, which are absorbed and resynthesized triglycerides leading to obesity ultimately [3]. The inhibition of fat digestion is an approach for reducing lipid absorption which can reduce the chance of obesity.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

The Inhibitory Effect of Different Solvents Extracts from Walnut Shell (Juglans regia L.) on Pancreatic Lipase and Adipogenesis of 3T3-L1 Preadipocytes

Yang¹,

Chen²,

Zhao³

et al. 2014

JFNR

View full text Add to dashboard Cite

In this article, the effect of the solvents (water, methanol, ethanol, chloroform, N-butanol and ethyl acetate) on the extraction yields (EY), total flavonoids content (TFC), total phenols content (TPC) form walnut shell and the inhibitory effect of extracts on pancreatic lipase were analysed. The kinetics of enzyme inhibition and was investigated. The inhibitory effect of extracts on adipogenesis of 3T3-L1 preadipocytes was also investigated. The highest EY, TFC and TPC were achieved with N-butanol (4.54%), Ethyl acetate (80.40 mg QEs/g extract) and ethyl acetate (200.40 mg GAE/g extract). The order of inhibitory activity was methanol extract (ME) > ethyl acetate extract (EAE) > ethanol extract (EE) > water extract (WE) > N-butanol extract (NBE) > chloroform extract (CE), the inhibition ratios were 82.51%, 73.13%, 70.49%, 69.42%, 65.57% and 60.66%, respectively under the concentration of 500 μg/mL. The IC 50 values of WE, ME, EE, CE, NBE and EAE were 371.35, 308.91, 407.57, 196.50, 247.79 and 254.27 μg/mL respectively. The inhibitory types of walnut shell extracts were noncompetitive. All the extracts were non-toxic to 3T3-L1 preadipocytes. The ME, EE, CE, NBE and EAE have inhibitory effect on triglyceride accumulation of 3T3-L1 cells.

show abstract

Section: Kinetics Of Enzyme Inhibitionsupporting

confidence: 89%

Section: Pancreatic Lipase Inhibitory Activitymentioning

confidence: 88%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The Inhibitory Effect of Different Solvents Extracts from Walnut Shell (Juglans regia L.) on Pancreatic Lipase and Adipogenesis of 3T3-L1 Preadipocytes

Yang¹,

Chen²,

Zhao³

et al. 2014

JFNR

View full text Add to dashboard Cite

show abstract

“…Fluorescence spectroscopy is widely used to investigate the interaction between proteins and small molecule compounds [39]. When the excitation wavelength is set at 280 nm, the intrinsic fluorescence of protein at 340 nm is mainly contributed by tryptophan (Trp) and tyrosine (Tyr) residues.…”

Section: Fluorescence Quenching Of Lipase By the Inhibitorsmentioning

confidence: 99%

Screening of lipase inhibitors from Scutellaria baicalensis extract using lipase immobilized on magnetic nanoparticles and study on the inhibitory mechanism

et al. 2016

View full text Add to dashboard Cite

Scutellaria baicalensis is a traditional Chinese medicinal plant possessing a wide variety of biological activities. In this work, lipase immobilized on magnetic nanoparticles (LMNPs) was used as solid phase extract absorbent for screening of lipase inhibitors from this plant. Three flavonoids were found to bind to LMNPs and were identified as baicalin, wogonin, and oroxylin A by liquid chromatography-mass spectrometry (HPLC-MS). Their IC 50 values were determined to be 229.22 ± 12.67, 153.71 ± 9.21, and 56.07 ± 4.90 μM, respectively. Fluorescence spectroscopy and molecular docking were used to probe the interactions between these flavonoids and lipase. All the flavonoids quenched the fluorescence of lipase statically by forming new complexes, implying their affinities with the enzyme. The thermodynamic analysis suggested that van der Waals force and hydrogen bond were the main forces between wogonin and lipase, while hydrophobic force was the main force for the other two flavonoids. The results from a molecular docking study further revealed that all of them could insert into the pocket of lipase binding to a couple of amino acid residues.

show abstract

Myricetin and dihydromyricetin as JNK1 inhibitors protect against ROS‐mediated oxidative stress and apoptosis

Hao,

Song,

et al. 2024

eFood

View full text Add to dashboard Cite

Reactive oxygen species (ROS)‐mediated oxidative stress and apoptosis contribute to the pathogenesis and pathological conditions of various diseases induced by food and environmental pollutants. Myricetin and dihydromyricetin, as natural flavonoids, can counteract oxidative damage, apoptosis, and associated diseases. Molecular docking analysis, ELISA, fluorescence spectroscopy, and Western blot techniques were applied to examine the potential of the two flavonoids as effective therapeutics for ROS‐mediated diseases and the mechanism(s) underlying their protective effects against ethanol/H2O2‐induced damage. In Kunming mice and HepG2 cells, myricetin and dihydromyricetin inhibited ethanol/H2O2‐induced liver damage, ROS accumulation, and oxidative stress and apoptosis. Molecular docking showed that myricetin and dihydromyricetin interacted with JNK1 via hydrophobic interactions and hydrogen bonds, thereby exerting their protective effects. The significant differences in their binding patterns to JNK1 resulted from the different bond types between C2 and C3 on their C ring. Myricetin and dihydromyricetin likely protected liver injury via counteracting ROS/JNK1‐mediated oxidative stress and apoptosis. The finding that the suppression of ROS‐mediated oxidative stress and apoptosis by flavonoids via interacting with functional proteins, such as kinase, may represent an alternative strategy for treating excessive ROS‐induced health problems.

show abstract

Probing the interaction between 3 flavonoids and pancreatic lipase by methods of fluorescence spectroscopy and enzymatic kinetics

Cited by 56 publications

References 17 publications

The Inhibitory Effect of Different Solvents Extracts from Walnut Shell (<i>Juglans regia </i><i>L.</i>) on Pancreatic Lipase and Adipogenesis of 3T3-L1 Preadipocytes

The Inhibitory Effect of Different Solvents Extracts from Walnut Shell (<i>Juglans regia </i><i>L.</i>) on Pancreatic Lipase and Adipogenesis of 3T3-L1 Preadipocytes

Screening of lipase inhibitors from Scutellaria baicalensis extract using lipase immobilized on magnetic nanoparticles and study on the inhibitory mechanism

Myricetin and dihydromyricetin as JNK1 inhibitors protect against ROS‐mediated oxidative stress and apoptosis

Contact Info

Product

Resources

About