Probing the Impact of Gamma-Irradiation on the Metabolic State of Neural Stem and Precursor Cells Using Dual-Wavelength Intrinsic Signal Two-Photon Excited Fluorescence

Krasieva, Tatiana B.; Giedzinski, Erich; Tran, Katherine K.; Lan, Mary L.; Limoli, Charles L.; Tromberg, Bruce J.

doi:10.1142/s1793545811001629

Cited by 3 publications

(1 citation statement)

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“…An increase in early larvae survival following embryonic exposure to radiation has also been seen for zebrafish, and is thought to be associated with better use of yolk sac reserves relative to nonirradiated embryos (Simon et al ). It is also possible that cumulative exposure to chronic low‐dose irradiation triggered an increase in DNA repair pathways and reduced mitochondrial oxidative metabolism (Krasieva et al ) or stress response mechanisms (e.g., increasing constitutive heat shock proteins and/or inducible heat shock protein production), which may help prepare the embryos for future stressors (Jolly and Meyer ). Thus chronic low‐dose irradiation may not prominently impact round whitefish during embryonic development, but it could be beneficial for posthatch survival, and consequently, recruitment to the next year.…”

Section: Discussionmentioning

confidence: 99%

Impacts of temperature, morpholine, and chronic radiation on the embryonic development of round whitefish (Prosopium cylindraceum)

Lim

Manzon

Somers

et al. 2018

Enviro Toxic and Chemistry

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During incubation, round whitefish embryos may experience fluctuating or elevated temperatures from natural (e.g., seasonal temperature changes) and/or anthropogenic sources. Anthropogenic sources like once-through cooling discharges from nuclear power plants can also expose embryos to chemicals (e.g., morpholine) and/or radiation. To examine the effects of these potential stressors on embryogenesis, round whitefish were incubated under fluctuating or constant temperatures, with morpholine or Cs gamma rays. We report the percentage of prehatch and posthatch mortality, developmental rate, hatch dynamics, and morphometrics at 4 development stages. Embryos reared at constant temperatures had delayed developmental stage onset and median hatch, higher mortality at constant 8 °C, and lower mortality at ≤5 °C, compared with embryos reared under seasonal temperature regimes. Embryos incubated with ≥500 mg L morpholine (>200× regulatory limits) had advanced hatch, reduced body size, and increased prehatch (100% at 1000 mg L ) and posthatch (≈95% at 500 mg L ) mortality compared with controls. Relative to controls, embryos irradiated with ≥0.16 mGy/d had larger body mass early in development, and all irradiated embryos had decreased posthatch mortality; the lowest dose was >300× discharge limits. Our study suggests that fluctuating or elevated temperatures and high-dose morpholine can alter development rate, hatch dynamics, and growth, and/or increase mortality compared with embryos reared at constant temperatures of ≤5 °C; conversely, low-dose irradiation had transient developmental effects but may benefit early posthatch survival. Environ Toxicol Chem 2018;37:2593-2608. © 2018 SETAC.

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Section: Discussionmentioning

confidence: 99%