Probing the functional role and localization of Escherichia coli ribosomal protein L16 with a monoclonal antibody

Nag, Bishwajit; Glitz, Dohn G.; Tewari, D S; Traut, Robert R.

doi:10.1016/s0021-9258(18)99135-0

Cited by 8 publications

(1 citation statement)

References 38 publications

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“…(1988). Sources for other placements: L16, Nag et al (1991); puromycin and p-azidopuromycin, Olson et al (1982Olson et al ( , 1985; 5S rRNA, Shatsky et al (1980), Stoffler-Meilicke et al (1981), and Evstafieva et al (1985.…”

Section: Acknowledgmentmentioning

confidence: 99%

Ribosomal Components Neighboring the 2475 Loop in Escherichia coli 50S Subunits

Muralikrishna¹,

Cooperman²

1995

Biochemistry

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We report the synthesis of a radioactive, photolabile oligodeoxyribonucleotide probe and its exploitation in identifying 50S ribosomal subunit components neighboring its target site in 23S rRNA. The probe is complementary to 23S rRNA nucleotides 2475-2483, a single-stranded sequence (the 2475 loop) near the peptidyltransferase center of Escherichia coli ribosomes. On photolysis in the presence of 50S subunits, it site-specifically incorporates into proteins L1, L13, L16, L32, and L33 and into 23S rRNA nucleotides G2470, A2471, and G2472. These results provide clear evidence that C2475 in 23S rRNA is within 21 A (the distance between C2475 and the photogenerated nitrene) of proteins L1, L13, L16, L32, and L33. The implications of these results for the evolving model of the internal structure of the 50S subunit are considered.

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Section: Acknowledgmentmentioning

confidence: 99%

Ribosomal Components Neighboring the 2475 Loop in Escherichia coli 50S Subunits

Muralikrishna¹,

Cooperman²

1995

Biochemistry

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Gene expression modulation by chalcopyrite and bornite in Acidithiobacillus ferrooxidans

et al. 2010

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Acidithiobacillus ferrooxidans is a mesophilic, acidophilic, chemolithoautotrophic bacterium that obtains energy from the oxidation of ferrous iron (Fe2+), elemental sulfur and reduced sulfur compounds. The industrial interest in A. ferrooxidans resides in its capacity to oxidize insoluble metal sulfides into soluble metal sulfates, thus allowing the recovery of the desired metals from low-grade sulfide ores. In the present work, RNA arbitrarily primed PCR (RAP-PCR) was performed to identify cDNAs differentially expressed in A. ferrooxidans cells grown in the presence of Fe2+ and cells maintained for 24 h in the presence of the copper sulfides bornite and chalcopyrite. Eighteen cDNAs corresponding to genes with known function were identified, and their relative expression was further characterized by real-time quantitative PCR. Bornite had a mild effect on the expression of the 18 genes analyzed. None of these genes was down-regulated and among the few genes up-regulated, it is worth mentioning lepA and def-2 that are involved in protein synthesis. Chalcopyrite presented the most significant changes. Five genes related to protein processing were down-regulated, and another 5 genes related to the transport system were up-regulated. The up- and down-regulation of these genes in the presence of bornite and chalcopyrite could be due to alterations in the ideal pH, presence of copper ions in solution and nutrient limitation. The results suggest that gene expression modulation might be important for the A. ferrooxidans early response to copper sulfides.

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