Probing the Folding Dynamics of Human Telomeric G-Quadruplex with Single-Molecule FRET

Abstract

Oxidative stress has been connected to aging, cancer and the development of many other diseases. Oxidative damage occurs while cellular oxidizing species concentration increases or antioxidant defenses decrease. One of the most common products of oxidative damage in DNA is 8-oxoguanine (8-oxoG).The frequent presence of 8-oxoguanine is due to the lowest oxidation potential of guanine amongst the four DNA bases. In particular, the guanine rich nature telomeric 3'-overhang sequence makes telomere more prone to the 8-oxoG damage. Therefore it provides an interesting region for studying the consequence of oxidative damage in DNA. We studied the effect of 8-oxoG lesion in human telomeric overhang which consist of repeats TTAGGG that can self-fold into G-quadruplex (GQ). The central guanine of triple-G was replaced

Dispersive approach to two-photon exchange in elastic electron-proton scattering

“…The parametrization in Eq. (58a) is compared with an alternative parametrization from the recent analysis by Zhou and Yang [14], which agrees with the data in the intermediate Using the Aznauryan parametrization [60,61] of the γ * N ∆ form factors, the TPE contributions from ∆ intermediate states to the generalized form factors F 1 , F 2 and G a are illustrated in Fig. 10 for both the imaginary and real parts.…”

“…The electric form factor G * E determines the difference g 2 − g 1 , while g 3 is sensitive to G * E and the Coulomb form factor G * C . In the present analysis, we take the Jones-Scadron form factors from the phenomenological parametrization by Aznauryan [60,61],…”

“…Using the Aznauryan parametrization [60,61] of the γ * N ∆ form factors, the TPE contributions from ∆ intermediate states to the generalized form factors F 1 , F 2 and G a are illustrated in Fig. 10 for both the imaginary and real parts.…”

“…the ∆ transition form factors are fit to the parametrization of Ref. [60,61], The form factors g 1 (Q 2 ), g 2 (Q 2 ), and g 3 (Q 2 ) can be obtained from simple combinations of these parametrizations, while still allowing for implementation in analytic form.…”