Probing the dynamic crosstalk of lysosomes and mitochondria with structured illumination microscopy

Xu, Xiuqiong; Qiu, Kangqiang; Tian, Zhiqi; Aryal, Chinta; Rowan, Fiona; Chen, Rui; Sun, Yujie; Diao, Jiajie

doi:10.1016/j.trac.2023.117370

Cited by 2 publications

(6 citation statements)

References 127 publications

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“…The xanthene probe Me-hNR was produced by a condensation reaction between intermediates I and III in the presence of perchloric acid. All intermediates and the final product were well characterized by 1 HNMR, 13 CNMR, and HR-MS (Figures S1−S12). Me-hNR molecules contained delocalized positive charges and were expected to have mitochondria targeting.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…The Supporting Information is available free of charge at https://pubs.acs.org/doi/10.1021/acs.analchem.3c05154. Materials and methods; 1 HNMR, 13 CNMR, and HRMS spectra; photophysical data; stability data; and imaging data (PDF)…”

Section: * Sı Supporting Informationmentioning

confidence: 99%

“…1 H NMR (400 MHz, Chloroform-d) δ (ppm): 7.93 (d, J = 8.8 Hz, 1H), 6.57 (d, J = 8.8 Hz, 1H), 6.36 (s, 1H), 3.44 (q, J = 6.8 Hz, 4H), 2.87 (t, J = 5.6 Hz, 2H), 2.57 (t, J = 6.1 Hz, 2H), 2.10 (p, J = 5.5 Hz, 2H), 1.22 (t, J = 6.9 Hz, 6H). 13 Synthesis of Intermediate II. 3-Hydroxy-N,N-diethylaniline (1.0 g, 6.06 mmol), and CH 2 (COOPh) 2 (1.55 g, 6.06 mmol) were added to anhydrous toluene (20 mL).…”

Section: ■ Conclusionmentioning

confidence: 99%

“…1 H NMR (400 MHz, DMSO-d6) δ (ppm): 11.93 (s, 1H), 7.56 (d, J = 8.8 Hz, 1H), 6.67 (d, J = 8.9 Hz, 1H), 6.45 (s, 1H), 5.25 (s, 1H), 3.43 (q, J = 6.5 Hz, 4H), 1.13 (t, J = 6.6 Hz, 6H). 13 (20 mL). The mixture was stirred at 140 °C for 5 h. After the mixture cooled to room temperature, ammonia was added to the mixture to adjust the pH to 7.…”

Section: ■ Conclusionmentioning

confidence: 99%

“…The Supporting Information is available free of charge at https://pubs.acs.org/doi/10.1021/acs.analchem.3c05154. Materials and methods; 1 HNMR, 13…”

Section: * Sı Supporting Informationmentioning

confidence: 99%

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Ultrabright Xanthene Fluorescence Probe for Mitochondrial Super-Resolution Imaging

Wu,

Zhang,

Sha

et al. 2024

Anal. Chem.

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Mitochondria are important organelles that provide energy for cellular physiological activities. Changes in their structures may indicate the occurrence of diseases, and the super-resolution imaging of mitochondria is of great significance. However, developing fluorescent probes for mitochondrial super-resolution visualization still remains challenging due to insufficient fluorescence brightness and poor stability. Herein, we rationally synthesized an ultrabright xanthene fluorescence probe Me-hNR for mitochondria-specific superresolution imaging using structured illumination microscopy (SIM). The rigid structure of Me-hNR provided its ultrahigh fluorescence quantum yield of up to 0.92 and ultrahigh brightness of up to 16,000. Occupying the para-position of the O atom in the xanthene skeleton by utilizing the smallest methyl group ensured its excellent stability. The study of the photophysical process indicated that Me-hNR mainly emitted fluorescence via radiative decay, and nonradiative decay and inter-system crossing were rare due to the slow nonradiative decay rate and large energy gap (ΔE st = 0.55 eV). Owing to these excellent merits, Me-hNR can specifically light up mitochondria at ultralow concentrations down to 5 nM. The unprecedented spatial resolution for mitochondria with an fwhm of 174 nm was also achieved. Therefore, this ultrabright xanthene fluorescence probe has great potential in visualizing the structural changes of mitochondria and revealing the pathogenesis of related diseases using SIM.

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Section: ■ Results and Discussionmentioning

confidence: 99%

Section: * Sı Supporting Informationmentioning

confidence: 99%

Section: ■ Conclusionmentioning

confidence: 99%

Section: ■ Conclusionmentioning

confidence: 99%

“…The Supporting Information is available free of charge at https://pubs.acs.org/doi/10.1021/acs.analchem.3c05154. Materials and methods; 1 HNMR, 13…”

Section: * Sı Supporting Informationmentioning

confidence: 99%

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Ultrabright Xanthene Fluorescence Probe for Mitochondrial Super-Resolution Imaging

Wu,

Zhang,

Sha

et al. 2024

Anal. Chem.

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Nanoscale insights into hematology: super-resolved imaging on blood cell structure, function, and pathology

Liu,

Tan,

Zheng

et al. 2024

J Nanobiotechnol

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Fluorescence nanoscopy, also known as super-resolution microscopy, has transcended the conventional resolution barriers and enabled visualization of biological samples at nanometric resolutions. A series of super-resolution techniques have been developed and applied to investigate the molecular distribution, organization, and interactions in blood cells, as well as the underlying mechanisms of blood-cell-associated diseases. In this review, we provide an overview of various fluorescence nanoscopy technologies, outlining their current development stage and the challenges they are facing in terms of functionality and practicality. We specifically explore how these innovations have propelled forward the analysis of thrombocytes (platelets), erythrocytes (red blood cells) and leukocytes (white blood cells), shedding light on the nanoscale arrangement of subcellular components and molecular interactions. We spotlight novel biomarkers uncovered by fluorescence nanoscopy for disease diagnosis, such as thrombocytopathies, malignancies, and infectious diseases. Furthermore, we discuss the technological hurdles and chart out prospective avenues for future research directions. This review aims to underscore the significant contributions of fluorescence nanoscopy to the field of blood cell analysis and disease diagnosis, poised to revolutionize our approach to exploring, understanding, and managing disease at the molecular level. Graphical Abstract

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Probing the dynamic crosstalk of lysosomes and mitochondria with structured illumination microscopy

Cited by 2 publications

References 127 publications

Ultrabright Xanthene Fluorescence Probe for Mitochondrial Super-Resolution Imaging

Ultrabright Xanthene Fluorescence Probe for Mitochondrial Super-Resolution Imaging

Nanoscale insights into hematology: super-resolved imaging on blood cell structure, function, and pathology

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