“…Although these amounts may seem small, they are indeed significant. Volumetric changes involved in folding or unfolding induced in proteins by ligand binding are very modest ( Azam et al, 2021 , Azam et al, 2020 , Ball, 2017 , Brovchenko et al, 2010 , Kaur et al, 2018 , Pereira et al, 2006 , Persson and Halle, 2018 , Petřek et al, 2007 , Pfeiffer et al, 2008 ). According to Eq.…”