Probing strigolactone receptors in
            <i>Striga hermonthica</i>
            with fluorescence

Tsuchiya, Yuichiro; Yoshimura, Masahiko; Sato, Yoshikatsu; Kuwata, Keiko; Toh, Shigeo; Holbrook-Smith, Duncan; Zhang, Hua; McCourt, Peter; Itami, Kenichiro; Kinoshita, Toshinori; Hagihara, Shinya

doi:10.1126/science.aab3831

Cited by 231 publications

(298 citation statements)

References 30 publications

(15 reference statements)

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“…Intriguingly, a high number of KAI2/HTL genes are present in the P. patens genome compared with angiosperms but also with Selaginella and Marchantia (Delaux et al, 2012). A similar KAI2/HTL gene expansion is found in parasitic plants (Conn et al, 2015;Tsuchiya et al, 2015). Interestingly, in these species, it was suggested that some of these KAI2/HTL homologs could be SL receptors (Conn et al, 2015).…”

Section: The Origin and Evolution Of Sl Signalingmentioning

confidence: 71%

Strigolactone biosynthesis and signaling in plant development

et al. 2015

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Strigolactones (SLs), first identified for their role in parasitic and symbiotic interactions in the rhizosphere, constitute the most recently discovered group of plant hormones. They are best known for their role in shoot branching but, more recently, roles for SLs in other aspects of plant development have emerged. In the last five years, insights into the SL biosynthetic pathway have also been revealed and several key components of the SL signaling pathway have been identified. Here, and in the accompanying poster, we summarize our current understanding of the SL pathway and discuss how this pathway regulates plant development.

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Section: The Origin and Evolution Of Sl Signalingmentioning

confidence: 71%

Strigolactone biosynthesis and signaling in plant development

et al. 2015

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“…2 J-N). This site spatially overlaps with the cellular location where germination is initiated in Arabidopsis (12,32), and where the hormone strigolactone first acts to stimulate the germination of Striga seeds (33). Fate switching in the radicle is informed by the surrounding endosperm, where all decision-making components are also present (SI Appendix, Supplementary Fig.…”

Section: Resultsmentioning

confidence: 99%

Temperature variability is integrated by a spatially embedded decision-making center to break dormancy in Arabidopsis seeds

Topham

Taylor

Yan

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

107

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Plants perceive and integrate information from the environment to time critical transitions in their life cycle. Some mechanisms underlying this quantitative signal processing have been described, whereas others await discovery. Seeds have evolved a mechanism to integrate environmental information by regulating the abundance of the antagonistically acting hormones abscisic acid (ABA) and gibberellin (GA). Here, we show that hormone metabolic interactions and their feedbacks are sufficient to create a bistable developmental fate switch in Arabidopsis seeds. A digital single-cell atlas mapping the distribution of hormone metabolic and response components revealed their enrichment within the embryonic radicle, identifying the presence of a decision-making center within dormant seeds. The responses to both GA and ABA were found to occur within distinct cell types, suggesting cross-talk occurs at the level of hormone transport between these signaling centers. We describe theoretically, and demonstrate experimentally, that this spatial separation within the decision-making center is required to process variable temperature inputs from the environment to promote the breaking of dormancy. In contrast to other noise-filtering systems, including human neurons, the functional role of this spatial embedding is to leverage variability in temperature to transduce a fate-switching signal within this biological system. Fluctuating inputs therefore act as an instructive signal for seeds, enhancing the accuracy with which plants are established in ecosystems, and distributed computation within the radicle underlies this signal integration mechanism.seed | dormancy | signal integration | distributed control | variability P lant development is guided by the perception of diverse environmental cues and their integration into key transitions (1). One major decision in the life cycle of plants is when to commence flowering (2, 3). The other major decision is when to initiate a new plant (4). This decision is achieved through seed dormancy, an adaptive trait that determines where and when plants are established, and the entry and exit of plants into and out of ecosystems (4). The germination of seeds also represents the starting point for the vast majority of world agriculture, having great industrial, economic, and societal significance (5). During seed development, dormancy level is established in response to the environment experienced by the mother plant (6). This control is achieved through the quantitative regulation of genetically encoded regulatory factors, including the DOG1 locus (7, 8), and hormone abundance and sensitivity (9, 10). Following their release from the mother plant, the control of dormancy in seeds was proposed to be mediated by the activity of antagonistically acting factors (11). Later work identified this endogenous signal integration mechanism to consist of the antagonistically acting hormone abscisic acid (ABA) promoting dormancy and gibberellin (GA) promoting germination (9, 12). The relative abundance of ...

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“…It is possible that wholesale duplication of this signaling pathway in the vascular plant lineage gave rise to the D14 and SMXL6,7,8/D53 clades, and that subsequent coevolution of the receptor-target pairs created separate signaling pathways for SL and KAR/KL (Bennett and Leyser, 2014). Interestingly, this signaling system has evolved more recently at the receptor level in parasitic plants in the Orobanchaceae family, in which SL perception through KAI2 is the basis of hosttriggered germination (Conn et al, 2015;Toh et al, 2015;Tsuchiya et al, 2015).…”

Section: Evolutionary Diversification Of Butenolide Signaling Pathwaysmentioning

confidence: 99%

SMAX1-LIKE/D53 Family Members Enable Distinct MAX2-Dependent Responses to Strigolactones and Karrikins in Arabidopsis

et al. 2015

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The plant hormones strigolactones and smoke-derived karrikins are butenolide signals that control distinct aspects of plant development. Perception of both molecules in Arabidopsis thaliana requires the F-box protein MORE AXILLARY GROWTH2 (MAX2). Recent studies suggest that the homologous SUPPRESSOR OF MAX2 1 (SMAX1) in Arabidopsis and DWARF53 (D53) in rice (Oryza sativa) are downstream targets of MAX2. Through an extensive analysis of loss-of-function mutants, we demonstrate that the Arabidopsis SMAX1-LIKE genes SMXL6, SMXL7, and SMXL8 are co-orthologs of rice D53 that promote shoot branching. SMXL7 is degraded rapidly after treatment with the synthetic strigolactone mixture rac-GR24. Like D53, SMXL7 degradation is MAX2-and D14-dependent and can be prevented by deletion of a putative P-loop. Loss of SMXL6,7,8 suppresses several other strigolactone-related phenotypes in max2, including increased auxin transport and PIN1 accumulation, and increased lateral root density. Although only SMAX1 regulates germination and hypocotyl elongation, SMAX1 and SMXL6,7,8 have complementary roles in the control of leaf morphology. Our data indicate that SMAX1 and SMXL6,7,8 repress karrikin and strigolactone signaling, respectively, and suggest that all MAX2-dependent growth effects are mediated by degradation of SMAX1/SMXL proteins. We propose that functional diversification within the SMXL family enabled responses to different butenolide signals through a shared regulatory mechanism.

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Probing strigolactone receptors in Striga hermonthica with fluorescence

Cited by 231 publications

References 30 publications

Strigolactone biosynthesis and signaling in plant development

Strigolactone biosynthesis and signaling in plant development

Temperature variability is integrated by a spatially embedded decision-making center to break dormancy in Arabidopsis seeds

SMAX1-LIKE/D53 Family Members Enable Distinct MAX2-Dependent Responses to Strigolactones and Karrikins in Arabidopsis

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