Probing Regulatory Proteins for Vascular Contraction by Deoxyribonucleic Acid Microarray

Abstract

Background and ObjectivesThe heat-shock response modulates contractility of vascular smooth muscles. With complementary deoxyribonucleic acid microarray, we tried to identify the novel genes that are involved in the regulation of vascular contraction after heat shock.Materials and MethodsHuman radial artery strips were mounted in organ baths, exposed at 42℃ for 45 minutes, and returned to equilibrate at 37℃. This study examined gene expression profile associated with heat-shock response in radial arteries of patients with hyperlipidemia by using a microarray that contained 5763 human cDNA. The results of microarray hybridization experiments from the radial arteries of 4 different subjects were analyzed and classified by the cluster program.ResultsAmong these differentially-expressed genes, Hsp70, Hsp10, αB-crystallin, and Hsp60 were significantly increased by the heat shock response. Of non-HSP genes, 15 genes increased, while 22 genes decreased. Among these 37 genes, αB-crystallin (CRYAB) (up 1.92-fold), myosin, light polypeptide kinase transcript variant 8, 6 (up 1.70-fold, up 1.68-fold), catenin (cadherin-associated protein, alpha-like 1) (down-0.57 fold) and tropomyosin 3 (down 0.68-fold) were thought to be related with the contraction. Real-time quantitative polymerase chain reaction showed that Hsp70, Hsp10 and αB-crystallin were significantly increased.ConclusionGene expression profile by heat shock provides information about genes implicated in augmentation of vascular contraction after heat shock.

Differential Gene Expression of Cardiac Ion Channels in Human Dilated Cardiomyopathy

“…Moreover, there have been many studies that have used this method to examine expression levels of genes related to the EC process that occurs in muscle cells [27-29]. Besides, there are studies analyzing failing and non-failing human hearts establishing gender differences in electrophysiological gene expression, and using these data to predict the electrophysiological remodeling [30,31].…”

“…Microarray experiments are a suitable method for analyzing the global expression of genes involved in human diseases, such as HF, showing alterations in gene expression profiles [ 23 - 26 ]. Moreover, there have been many studies that have used this method to examine expression levels of genes related to the EC process that occurs in muscle cells [ 27 - 29 ]. Besides, there are studies analyzing failing and non-failing human hearts establishing gender differences in electrophysiological gene expression, and using these data to predict the electrophysiological remodeling [ 30 , 31 ].…”

Bases Genómicas Y Celulares De La Contracción Ventricular. Papel Del Retículo Endoplásmico Y Los Canales Iónicos en La Insuficiencia Cardiaca Humana

“…La tecnología de microarrays proporciona un método apropiado para el análisis de la expresión global de genes implicados en enfermedades humanas como la IC, mostrando las alteraciones presentes en los perfiles de expresión génica (187)(188)(189)(190). Además, existen numerosos estudios que utilizan este método para examinar los niveles de expresión de genes relacionados con el proceso de contracción de las células musculares (191)(192)(193), así como estudios que analizan, en corazones patológicos y sanos, las diferencias entre géneros en cuanto a la expresión génica de moléculas implicadas en electrofisiología (138,194). La herramienta de clasificación funcional de genes DAVID, resume la principal implicación biológica de estos grupos de genes y permite ordenar amplias listas de genes en grupos funcionalmente relacionados adjudicando una puntuación de enriquecimiento.…”