Probing mutual interactions between <i>Pseudomonas aeruginosa</i> and <i>Candida albicans</i> in a biofabricated membrane-based microfluidic platform

Pham, Le Hoang Phu; Colon-Ascanio, Mariliz; Ou, Jin; Ly, Khanh L.; Piao, Hongyu; Choy, John S.; Luo, Xiaolong

doi:10.1039/d2lc00728b

Cited by 3 publications

(2 citation statements)

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“…Antibiotics with strong interaction with alginate hydrogel such as tetracycline [ 48 ] were excluded. It has been previously shown that small molecules such as fluorescein, glucose, peptides, and antibiotics can freely diffuse through the alginate hydrogels [ 34 , 35 , [49] , [50] , [51] ] as the pore size of the alginate hydrogel prepared with 1% w/v should be around tens of nanometers [ 52 , 53 ]. Together, these observations suggest that the alginate hydrogel did not limit the diffusion of the selected antibiotics.…”

Section: Resultsmentioning

confidence: 99%

Dissolvable alginate hydrogel-based biofilm microreactors for antibiotic susceptibility assays

Pham

Colon-Ascanio

et al. 2023

Biofilm

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Section: Resultsmentioning

confidence: 99%

Dissolvable alginate hydrogel-based biofilm microreactors for antibiotic susceptibility assays

Pham

Colon-Ascanio

et al. 2023

Biofilm

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“…Importantly, the pH-dependent sol–gel transition of chitosan-based on amine chemistry ( Figure 1 A) enables a facile and reversible biofabrication of chitosan membrane-integrated microchips by simply manipulating the pH of the surrounding aqueous environment [ 20 , 24 ]. Using this amine chemistry of chitosan, Luo’s group integrated chitosan membranes in microfluidic platforms with flows by creating localized pH gradients at the flow interface for numerous applications ranging from chemical gradient generation [ 20 ], chemotaxis [ 25 ], and chemotropism [ 26 ] to cellular signaling and communications [ 27 , 28 , 29 ]. On the other hand, Payne’s group has pioneered the use of electrical stimuli to form spatiotemporally programmable chitosan membranes on the cathode surface, which have been used as bioelectronic sensors [ 30 , 31 ], protective barriers against redox reactions [ 32 ], and antimicrobial films [ 33 ].…”

Section: Introductionmentioning

confidence: 99%

Programmable Physical Properties of Freestanding Chitosan Membranes Electrofabricated in Microfluidics

Piao

Raub

et al. 2023

Membranes

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Microfluidic-integrated freestanding membranes with suitable biocompatibility and tunable physicochemical properties are in high demand for a wide range of life science and biological studies. However, there is a lack of facile and rapid methods to integrate such versatile membranes into microfluidics. A recently invented interfacial electrofabrication of chitosan membranes offers an in-situ membrane integration strategy that is flexible, controllable, simple, and biologically friendly. In this follow-up study, we explored the ability to program the physical properties of these chitosan membranes by varying the electrofabrication conditions (e.g., applied voltage and pH of alginate). We found a strong association between membrane growth rate, properties, and fabrication parameters: high electrical stimuli and pH of alginate resulted in high optical retardance and low permeability, and vice versa. This suggests that the molecular alignment and density of electrofabricated chitosan membranes could be actively tailored according to application needs. Lastly, we demonstrated that this interfacial electrofabrication could easily be expanded to produce chitosan membrane arrays with higher uniformity than the previously well-established flow assembly method. This study demonstrates the tunability of the electrofabricated membranes’ properties and functionality, thus expanding the utility of such membranes for broader applications in the future.

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High‐Resolution Large‐Area Image Analysis Deciphers the Distribution of Salmonella Cells and ECM Components in Biofilms Formed on Charged PEDOT:PSS Surfaces

Ray,

Löffler,

Richter‐Dahlfors

2024

Advanced Science

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Biofilms, comprised of cells embedded in extracellular matrix (ECM), enable bacterial surface colonization and contribute to pathogenesis and biofouling. Yet, antibacterial surfaces are mainly evaluated for their effect on bacterial cells rather than the ECM. Here, a method is presented to separately quantify amounts and distribution of cells and ECM in Salmonella biofilms grown on electroactive poly(3,4‐ethylenedioxythiophene):polystyrenesulfonate (PEDOT:PSS). Within a custom‐designed biofilm reactor, biofilm forms on PEDOT:PSS surfaces electrically addressed with a bias potential and simultaneous recording of the resulting current. The amount and distribution of cells and ECM in biofilms are analyzed using a fluorescence‐based spectroscopic mapping technique and fluorescence confocal microscopy combined with advanced image processing. The study shows that surface charge leads to upregulated ECM production, leaving the cell counts largely unaffected. An altered texture is also observed, with biofilms forming small foci or more continuous structures. Supported by mutants lacking ECM production, ECM is identified as an important target when developing antibacterial strategies. Also, a central role for biofilm distribution is highlighted that likely influences antimicrobial susceptibility in biofilms. This work provides yet a link between conductive polymer materials and bacterial metabolism and reveals for the first time a specific effect of electrochemical addressing on bacterial ECM formation.

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Probing mutual interactions between Pseudomonas aeruginosa and Candida albicans in a biofabricated membrane-based microfluidic platform

Abstract: Microbes are typically found in multi-species (polymicrobial) communities. Cooperative and competitive interactions between species, mediated by diffusible factors and physical contact, leads to highly dynamic communities that undergo changes in...

Cited by 3 publications

References 54 publications

Dissolvable alginate hydrogel-based biofilm microreactors for antibiotic susceptibility assays

Dissolvable alginate hydrogel-based biofilm microreactors for antibiotic susceptibility assays

Programmable Physical Properties of Freestanding Chitosan Membranes Electrofabricated in Microfluidics

High‐Resolution Large‐Area Image Analysis Deciphers the Distribution of Salmonella Cells and ECM Components in Biofilms Formed on Charged PEDOT:PSS Surfaces

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