2014
DOI: 10.1111/tpj.12691
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Probing formation of cargo/importin‐α transport complexes in plant cells using a pathogen effector

Abstract: Importin-αs are essential adapter proteins that recruit cytoplasmic proteins destined for active nuclear import to the nuclear transport machinery. Cargo proteins interact with the importin-α armadillo repeat domain via nuclear localization sequences (NLSs), short amino acids motifs enriched in Lys and Arg residues. Plant genomes typically encode several importin-α paralogs that can have both specific and partially redundant functions. Although some cargos are preferentially imported by a distinct importin-α i… Show more

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Cited by 44 publications
(66 citation statements)
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“…Like TN13, neither SNC1 nor EDS1 or other known defense regulators were identified in MOS6 affinity purifications from Hpa Noco2‐infected tissues. We also did not identify the known MOS6 interactor HaRxL106 (Wirthmueller et al ., ) or other Hpa Noco2 effector proteins. Generally, this might be because of the fact that the interactions of α‐importins with their cargos are transient (e.g.…”
Section: Discussionmentioning
confidence: 99%
“…Like TN13, neither SNC1 nor EDS1 or other known defense regulators were identified in MOS6 affinity purifications from Hpa Noco2‐infected tissues. We also did not identify the known MOS6 interactor HaRxL106 (Wirthmueller et al ., ) or other Hpa Noco2 effector proteins. Generally, this might be because of the fact that the interactions of α‐importins with their cargos are transient (e.g.…”
Section: Discussionmentioning
confidence: 99%
“…Transgenic Arabidopsis plants expressing yellow fluorescent protein (YFP)-tagged HaRxL106 have been described (Wirthmueller et al, 2015). To generate transgenic lines expressing 3xHA-StrepII (HS):HaRxL106, RFP:HaRxL106, RFP:NLS: HaRxL106DC, and RFP:HaRxL106-Cterm58 (all Hpa Emoy2) we used the following previously described pENTR plasmids: pENTR4-HaRxL106, M followed by HaRxL106 amino acids I 25 -S 285 (Fabro et al, 2011), pENTR/D-TOPO-SV40NLS: HaRxL106DC, sequence APKKKRKV followed by HaRxL106 amino acids I 25 -G 229 , and pENTR/D-TOPO-HaRxL106-Cterm58, HaRxL106 amino acids G 228 -S 285 (Wirthmueller et al, 2015). Plasmids pXCSG-HS and pH7WGR2 were recombined with the aforementioned pENTR plasmids using Gateway ® LR clonase II to generate HS-and red fluorescent protein (RFP)-tagged versions of the HaRxL106 constructs respectively.…”
Section: Generation Of Transgenic Arabidopsis Linesmentioning
confidence: 99%
“…HaRxL106 is actively transported into nuclei of plant cells (Wirthmueller et al, 2015). Given that NPR1 is an important nuclear signal integrator of the SA pathway, we tested whether HaRxL106 affects NPR1 localization or protein levels.…”
Section: Effector Harxl106 Attenuates Npr1-dependent Defense Activationmentioning
confidence: 99%
See 1 more Smart Citation
“…Nuclear import occurs in a highly dynamic and rapid manner, thus the interactions of importin α with cargoes in the cytoplasm are usually not easily visualized or detected in cells. In addition, importin α superfamily contains several distinct isoforms (seven in human) that have both specific and partially redundant functions, and it remains unclear how cargoes are preferentially recognized by a distinct importin α . Thus, to test the efficiency and application of Mito‐docking, we tried to determine whether the method can capture this kind of dynamic interactions occurring in nuclear transport by detecting the interaction of six isoforms of human importin α (α1, α3, α4, α5, α6, and α7) with the nuclear localization signal (NLS) of wild‐type simian virus 40 (SV40) (NLS wt ).…”
Section: The Interactions Of Different Importin α Isoforms With Sv40‐mentioning
confidence: 99%