Probestin, a new inhibitor of aminopeptidase M, produced by Streptomyces azureus MH663-2F6. I. Taxonomy, production, isolation, physico-chemical properties and biological activities.

Aoyagi, Takaaki; Yoshida, Shigemi; Nakamura, Yasushi; Shigihara, Yasuhiro; Hamada, Masa; Takeuchi, Tomio

doi:10.7164/antibiotics.43.143

Cited by 66 publications

(24 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Although H9 and HUT78 cells express neutral aminopeptidase activities equal to or higher than U937, respectively, only a minor fraction of the aminopeptidase activity in T cells really originates from the APN gene. This could be concluded from the lack of inhibition of the enzymatic activity by the antibody WM15, strongly decreasing APN activity [21] and the low inhibition of enzymatic activity by the APNspecific inhibitor probestin [22]. Summarizing these data, it could be concluded that only 5 20% of total neutral aminopeptidase activities in T cells represent membrane-bound APN.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Quantification of aminopeptidase N mRNA in T cells by competitive PCR

Wex

Lendeckel

Wex³

et al. 1995

FEBS Letters

View full text Add to dashboard Cite

the occurrence of neutral aminopeptidase activity on these cells [13][14][15][16]18]. These results suggest that the aminopeptidase N (CD 13) is expressed in lymphoid cells too.To address this assumption and to investigate aminopeptidase N-gene expression in lymphoid cells, we have created a competitive RT-PCR of APN mRNA. Furthermore, neutral aminopeptidase activity of T cell lines was characterized in more detail to answer the question whether their aminopeptidase activity could be attributed to APN. Materials and methods Cloning and partial deletion of an APN gene fragmentThe 1865-bp EcoRI fragment of the APN cDNA (kindly provided by A.T. Look) was cloned into the plasmid pSPTI9 (Boehringer Mannheim). The deletion of the APN cDNA fragment was achieved by BAL31 nuclease (USB) using the unique DraIII restriction site of the APN cDNA. Extent and position of the deletion were determined by sequencing using the 'cycle sequencing kit' (USB) and the DIGlabelled APN-specific primer A1. The sample was loaded on a 8% sequencing gel and electrophoretically separated by the direct blotting system GATC (MWG Biotec) as recommended by the manufacturer.

show abstract

Section: Discussionmentioning

confidence: 99%

“…All measurements were made in triplicate as previously described [13]. The presence of APN was detected using APN-specific effectors, such as the anti-CD 13 monoclonal antibody WMI5 [21] and the APN-specific inhibitor probestin [22].…”

Section: Enzymatic Assaymentioning

confidence: 99%

Quantification of aminopeptidase N mRNA in T cells by competitive PCR

Wex

Lendeckel

Wex³

et al. 1995

FEBS Letters

View full text Add to dashboard Cite

show abstract

“…It is also present in phebestin (167), an inhibitor of aminopeptidase N [157], and in probestin (168) [158], an inhibitor of aminopeptidase M [159] (Figure 4.26 Ahoa (159) is present in the cyclic heptapeptide nostophycin (169) isolated from a cyanobacterium of the genus Nostoc [160], while Apoa/Ahmp (160) and its p-bromophenyl derivative Aboa (161) occur in theonegramide [161], the theonellamides A (170) and D (171) [17,18], and theopalauamide [162], which are bicyclic dodecapeptides isolated from marine sponges belonging to the genus Theonella (Figure 4.27).…”

Section: Biosynthesis Of B-amino Acids J139mentioning

confidence: 99%

β‐Amino Acid Biosynthesis

Spiteller

2009

Amino Acids, Peptides and Proteins in Organic Chemistry

View full text Add to dashboard Cite

“…Up to now, a quantity of APN inhibitors such as Bestatin, Probestin 8 , Amatatin 9 , Prebestin 8 , Lapstatin 10 , AHPA-Val 11 have been reported. The first APN inhibitor Bestatin was isolated from a culture filtrate of Streptomyces olivoreticuli in 1976 12 , and now generally used as the positive control in the assay of small molecule APN inhibitors.…”

Section: Introductionmentioning

confidence: 99%