Probe-specific loop-mediated isothermal amplification magnetogenosensor assay for rapid and specific detection of pathogenic Leptospira

Najian, A.B. Nurul; Foo, Phiaw Chong; İsmail, Nabilah; Low, Kim-Fatt; Yean, Chan Yean

doi:10.1016/j.mcp.2019.03.001

Cited by 16 publications

(9 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…(a) Schematic representation of a LAMP assay combined with DNAzyme for naked‐eye color observation—positive samples develop a greenish color, whereas the negatives remain colorless (diagram from Zhu et al., 2017). (b) Representation of the magnetosensor, with the result interpretation—negative samples (brown) and positive ones (dark green) (diagram from Nurul Najian et al., 2019)…”

Section: Naked‐eye Detectionmentioning

confidence: 99%

“…Similar approaches have been reported for other pathogens (Liu et al., 2018; Zhu et al., 2017). Just like DNAzymes mimicking HRP, this enzyme can be used directly, as demonstrated in the study of Nurul Najian et al., where they also implemented magnetic nanoparticles to concentrate the LAMP amplicons, for a later naked‐eye detection adding the HRP along with the corresponding substrates previously mentioned (Nurul Najian et al., 2019). The limitation of these procedures relies on the need to open the reaction tubes in order to perform the colorimetric detection (see Figure 10a,b).…”

Section: Naked‐eye Detectionmentioning

confidence: 99%

See 1 more Smart Citation

Naked‐eye detection strategies coupled with isothermal nucleic acid amplification techniques for the detection of human pathogens

Garrido‐Maestu

Prado

2022

Comp Rev Food Sci Food Safe

View full text Add to dashboard Cite

Nucleic acid amplification‐based techniques have gained acceptance by the scientific, and general, community as reference methodologies for many different applications. Since the development of the gold standard of these techniques, polymerase chain reaction (PCR), back in the 1980s many improvements have been made, and alternative techniques emerged reporting improvements over PCR. Among these, isothermal amplification approaches resulted of particular interest as could overcome the need of specialized equipment to accurately control temperature changes, but it was after year 2000 that these techniques have flourished in a huge number of novel alternatives with many different degrees of complexities and requirements. An added value is their possibility to be combined with many different naked‐eye detection strategies, simplifying the resources needed, allowing to reduce cost, and serving as the basis for novel developments of lab‐on‐chip systems, and miniaturized devices, for point‐of‐care testing. In this review, we will go over different types of naked‐eye detection strategies, combined with isothermal amplification. This will provide the readers up‐to‐date information for them to select the most appropriate strategies depending on the particular needs and resources for their experimental setup.

show abstract

Section: Naked‐eye Detectionmentioning

confidence: 99%

Section: Naked‐eye Detectionmentioning

confidence: 99%

Naked‐eye detection strategies coupled with isothermal nucleic acid amplification techniques for the detection of human pathogens

Garrido‐Maestu

Prado

2022

Comp Rev Food Sci Food Safe

View full text Add to dashboard Cite

show abstract

“…LAMP is widely used as a screening technique, because it can be conducted at a constant temperature and is highly efficient and specific. LAMP has been used for identification of Mycobacterium tuberculosis, herpes, severe acute respiratory syndrome, anthrax, human and avian influenza viruses, and other pathogens [12,13]. LAMP was able to detect Leptospira DNA at a concen tration as low as 200 pg/ml.…”

Section: Amplification Techniques That Increase Analytical Signal By mentioning

confidence: 99%

“…LAMP was able to detect Leptospira DNA at a concen tration as low as 200 pg/ml. The specificity of detection was 100%, as evaluated with 172 bacterial strains [12].…”

Section: Amplification Techniques That Increase Analytical Signal By mentioning

confidence: 99%

Isothermal Nucleic Acid Amplification Techniques and Their Use in Bioanalysis

Bodulev

Sakharov

2020

Biochemistry Moscow

View full text Add to dashboard Cite

“…As an alternative to PCR-based analysis, LAMP has many significant advantages. Firstly, LAMP has higher specificity and efficiency than PCR by using four specially designed primers for six different regions of the target gene, which enables the amplification to be completed within 1 h [18][19][20]. Secondly, LAMP can amplify DNA in isothermal conditions without any thermal cycler by using the Bst polymerase enzyme.…”

Section: Introductionmentioning

confidence: 99%

Development of Anti-Idiotypic Nanobody-Phage Based Immuno-Loop-Mediated Isothermal Amplification Assay for Aflatoxins in Peanuts

Lei

Han

Tang

et al. 2020

Toxins

View full text Add to dashboard Cite

Aflatoxin contamination in agricultural products has posed serious health hazards and brought huge economic loss in the food and feed industries. Monitoring aflatoxins in various foods and feeds has become a crucial means to protect public health. This study aimed to report an immuno-loop-mediated isothermal amplification (iLAMP) assay by using an anti-idiotypic nanobody-phage for on-site and rapid detection of aflatoxin in real samples. The iLAMP method was developed on the basis of a competitive immunoassay and LAMP reaction performed in a simple water bath. This method can provide visualized test results: violet color represents positive samples while sky blue represents negative. The visual detection limits of iLAMP for aflatoxin B1, B2, G1, and G2 in peanut samples were 1.6, 1.6, 3.2, and 16 μg/kg, respectively. The developed assay was verified with high performance liquid chromatography (HPLC) for the analysis of aflatoxins in peanuts, which demonstrated that the iLAMP method can be applied to the detection of aflatoxin in real samples. The novel iLAMP assay eliminates the need for aflatoxin conjugates, the antibody labeling process, and special equipment, and offers an alternative to existing methods with advantages of time-saving, cost-effectiveness, and ease-of-use.

show abstract

Probe-specific loop-mediated isothermal amplification magnetogenosensor assay for rapid and specific detection of pathogenic Leptospira

Cited by 16 publications

References 21 publications

Naked‐eye detection strategies coupled with isothermal nucleic acid amplification techniques for the detection of human pathogens

Naked‐eye detection strategies coupled with isothermal nucleic acid amplification techniques for the detection of human pathogens

Isothermal Nucleic Acid Amplification Techniques and Their Use in Bioanalysis

Development of Anti-Idiotypic Nanobody-Phage Based Immuno-Loop-Mediated Isothermal Amplification Assay for Aflatoxins in Peanuts

Contact Info

Product

Resources

About