Pro-inflammatory role of angiotensin II in mercuric chloride-induced nephropathy in rats

Peña, Caterina; Hernández-Fonseca, Juan Pablo; Rincón, Jaimar; Pedreáñez, Adriana; Viera, Ninoska; Mosquera, Jesús

doi:10.3109/1547691x.2012.699478

Cited by 7 publications

(6 citation statements)

References 41 publications

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“…The association of Ang II with the pathogenesis of HgCl 2 -induced nephronpathy has been previously documented Peña et al 2012). In this regard, a vasopressor role for glomerular Ang II during the early phase of HgCl 2 -induced nephropathy was reported .…”

Section: Introductionmentioning

confidence: 82%

“…Specifically, increased CD8 + cell infiltration in the interstitium and decreased infiltration in the glomeruli were found. While HgCl 2 has a potent nephrotoxic effect (mediated by Hg 2+ -glutathione complexes (Yanagisawa 1998), the effect is mainly in the proximal tubules, inducing acute tubular necrosis and interstitial inflammation (Yanagisawa 1998;Peña et al 2012). Our earlier studies noted that increased expression of immunological markers of inflammation (e.g.…”

Section: Discussionmentioning

confidence: 99%

“…Rats with no HgCl 2 treatment (Groups IV, V and VI) received, respectively, Enalapril, Losartan or normal saline only daily by gavage (500 ml). The Anti-Ang II doses used here were previously shown to be capable of diminishing pro-inflammatory events in different inflammatory models (Vargas et al 2012;Muñoz et al 2011;Peña et al 2012).…”

Section: Animals and Experimental Designmentioning

confidence: 99%

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Renal oxidative stress and renal CD8⁺ T-cell infiltration in mercuric chloride-induced nephropathy in rats: role of angiotensin II

Peña

Hernández-Fonseca

Pedreáñez

et al. 2015

Journal of Immunotoxicology

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Mercuric chloride (HgCl 2 ) induces kidney damage, in part, through oxidative stress. A role for angiotensin II (Ang II) in pro-inflammatory events in a model of acute HgCl 2 -induced nephropathy was reported. Ang II is a potent oxidative stress inducer; however, its role in oxidative/anti-oxidative events in HgCl 2 -induced nephropathy remains unknown. The aim of this study was to determine the role of Ang II in the oxidative stress and renal infiltration of CD8 + T-cells after an acute HgCl 2 intoxication. Three groups of Sprague Dawley rats were treated with a single subcutaneous dose of 2.5 mg/kg HgCl 2 : for 3 days prior to and for 4 days after that injection, rats in one group received Losartan (30 mg/kg), in another group Enalapril (30 mg/kg) or normal saline in the last group. Two other groups of drug-treated rats received saline in place of HgCl 2 . A final group of rats received saline in place of HgCl 2 and the test drugs. All treatments were via gastric gavage. At 96 h after the vehicle/HgCl 2 injection, blood and kidney samples were harvested. Renal sections were homogenized for measures of malondialdehyde (MDA), reduced glutathione (GSH) and catalase activity. Frozen sections were studied for the presence of superoxide anion (O À 2 ) and CD8 + T-cells. HgCl 2 -treated rats had increased interstitial and tubular expression of O À 2 , high levels of MDA, normal catalase activity and GSH content, increased levels of interstitial CD8 + T-cells and an increased percentage of necrotic tubules. Anti-Ang II treatments diminished the HgCl 2 -induced increases in interstitial O À 2 , CD8 + T-cells and tubular damage and increased catalase and GSH expression above that due to HgCl 2 alone; the HgCl 2 -induced high MDA levels were unaffected by the drugs. These data provide new information regarding the potential role of Ang II in the oxidative stress and renal CD8 + T-cell infiltration that occur during HgCl 2 nephropathy.ARTICLE HISTORY

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Section: Introductionmentioning

confidence: 82%

Section: Discussionmentioning

confidence: 99%

Section: Animals and Experimental Designmentioning

confidence: 99%

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Renal oxidative stress and renal CD8⁺ T-cell infiltration in mercuric chloride-induced nephropathy in rats: role of angiotensin II

Peña

Hernández-Fonseca

Pedreáñez

et al. 2015

Journal of Immunotoxicology

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“…In vivo titrations were performed in young male Sprague Dawley rats using groups of four rats, each inoculated intraperitoneally (ip) with 0.5 ml of log 10 dilutions of virus (10 -3 , 10 -4 , 10 -5 , 10 -6 ). Endpoints were determined by the method of Reed and Muench [13] and expressed as the median lethal dose (LD 50 [14,15] and PDTC [16,17] in rats. Thereafter, animals were divided in two groups.…”

Section: Virus Titration and Infectionmentioning

confidence: 99%

Role of angiotensin II in experimental Venezuelan equine encephalitis in rats

et al. 2015

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Venezuelan equine encephalitis (VEE) is a viral disease transmitted by mosquitoes. The inflammation induced by the VEE virus is associated with a high mortality rate in mice. Angiotensin II (Ang II), a pro-inflammatory molecule, is produced in the normal rat brain. There is no information about the role of this molecule in the inflammatory events occurring during VEE and the effect of inflammation on the mortality rate in VEE-virus-infected rats. This study was designed to determine the role of Ang II in VEE and to analyze the effect of inflammation on mortality in infected rats. Two groups of rats were studied: 1) Virus-infected animals and controls (n = 60) were treated with losartan (a blocker of the Ang II-AT1 receptor) or with pyrrolidine dithiocarbamate (PDTC, an inhibitor of NF-κB) or left untreated and analyzed for morbidity and mortality. 2) Animals treated using the same protocol (n = 30) were sacrificed at day 4 postinfection and analyzed by immunohistochemistry and histopathology and for cytokine production. Increased expression of Ang II, ICAM-1, ED-1 and cytokines (IL-1α, MCP-1, IL-6 and IL-10) in infected animals was observed. The main histopathology findings were dilated capillaries and capillaries with endothelial detachment. Losartan and PDTC reduced the expression of IL-1α, MCP-1, and IL-10, and the number of dilated capillaries and capillaries with endothelial detachment. Survival analysis showed that 100% mortality was reached earlier in infected rats treated with losartan (day 14) or PDTC (day 11) than in untreated animals (day 19). These findings suggest that Ang II plays a role in VEE and that brain inflammation is protective against viral infection.

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“…After binding of Ang II to its receptors, in particular AT1 (angiotensin II type 1 receptor: AT1 receptor), intracellular translocation of NF-kB to the nucleus occurs, inducing transcription of pro-inflammatory cytokines and production of oxygen-reactive species. Ang II-mediated inflammatory events can be inhibited by using AT-1 receptor antagonists or by inhibition of angiotensin I converting enzyme (ACE) [7][8][9][10][11]. Ang II is involved in the production of several cytokines, including interleukin-1 (IL-1) [12], and it is also involved in virus-induced respiratory pathogenesis [13][14][15].…”

Section: Introductionmentioning

confidence: 99%

Losartan and enalapril decrease viral absorption and interleukin 1 beta production by macrophages in an experimental dengue virus infection

et al. 2015

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The role of angiotensin II (Ang II) in dengue virus infection remains unknown. The aim of this study was to determine the effect of losartan, an antagonist of the angiotensin II type 1 receptor (AT1 receptor), and enalapril, an inhibitor of angiotensin I-converting enzyme (ACE), on viral antigen expression and IL-1β production in peritoneal macrophages infected with dengue virus type 2. Mice treated with losartan or enalapril and untreated controls were infected intraperitoneally with the virus, and macrophages were analyzed. Infection resulted in increased IL-1β production and a high percentage of cells expressing viral antigen, and this was decreased by treatment with anti-Ang II drugs, suggesting a role for Ang II in dengue virus infection.

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Pro-inflammatory role of angiotensin II in mercuric chloride-induced nephropathy in rats

Cited by 7 publications

References 41 publications

Renal oxidative stress and renal CD8⁺ T-cell infiltration in mercuric chloride-induced nephropathy in rats: role of angiotensin II

Renal oxidative stress and renal CD8⁺ T-cell infiltration in mercuric chloride-induced nephropathy in rats: role of angiotensin II

Role of angiotensin II in experimental Venezuelan equine encephalitis in rats

Losartan and enalapril decrease viral absorption and interleukin 1 beta production by macrophages in an experimental dengue virus infection

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Pro-inflammatory role of angiotensin II in mercuric chloride-induced nephropathy in rats

Cited by 7 publications

References 41 publications

Renal oxidative stress and renal CD8+ T-cell infiltration in mercuric chloride-induced nephropathy in rats: role of angiotensin II

Renal oxidative stress and renal CD8+ T-cell infiltration in mercuric chloride-induced nephropathy in rats: role of angiotensin II

Role of angiotensin II in experimental Venezuelan equine encephalitis in rats

Losartan and enalapril decrease viral absorption and interleukin 1 beta production by macrophages in an experimental dengue virus infection

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Renal oxidative stress and renal CD8⁺ T-cell infiltration in mercuric chloride-induced nephropathy in rats: role of angiotensin II

Renal oxidative stress and renal CD8⁺ T-cell infiltration in mercuric chloride-induced nephropathy in rats: role of angiotensin II