Pro-Inflammatory Oral Microbiota in Juvenile Spondyloarthritis: A Pilot Study

Abstract

The role of the microbiota in the pathogenesis of arthritis is gaining increasing attention. While multiple studies have queried the intestinal microbiota, very few have analyzed the contents of the oral microbiota. In this pilot study, we obtained salivary and sub-gingival specimens from a cohort of six healthy controls and five children with well-controlled spondyloarthritis (SpA) and performed 16S sequencing on bacteria obtained from both habitats. The Quantitative Insight into Microbial Ecology tool suite was used to generate operational taxonomic units, Phyloseq was used for diversity analyses, and DeSeq2 was used to compare abundances while adjusting for multiple comparisons. A repeat specimen was obtained from one subject during a flare. Clustering based upon diagnosis was observed from both habitats, with decreased alpha diversity seen within the plaque obtained from the patients vs. controls. Among the differentially abundant taxa were statistically significantly increased plaque Fusobacterium and salivary Rothia mucilaginosa among the patients compared to the controls. Additionally, the abundance of plaque Fusobacterium increased in one patient at the time of a flare. Our data suggest that the oral cavity may harbor bacteria involved in the pathogenesis of spondyloarthritis; additional studies are warranted.

The oral microbiome in autoimmune diseases: friend or foe?

“…Notably, the levels of Veillonellaceae , Brucella spp., and C. concisus were significantly higher in saliva samples from patients with AS, while the abundance of Streptococcus was decreased [ 207 ]. Stoll et al reported that the levels of salivary Rothia mucilaginosa and plaque-derived Fusobacterium were significantly increased in patients with spondyloarthritis [ 208 ], indicating AS is potentially an oral microbiome-driven disease.…”