Pro-Apoptotic Activity of French Polynesian Padina pavonica Extract on Human Osteosarcoma Cells

Abstract

Recently, seaweeds and their extracts have attracted great interest in the pharmaceutical industry as a source of bioactive compounds. Studies have demonstrated the cytotoxic activity of macroalgae towards different types of cancer cell models, and their consumption has been suggested as a chemo-preventive agent against several cancers such as breast, cervix and colon cancers. Reports relevant to the chemical properties of brown algae Padina sp. are limited and those accompanied to a comprehensive evaluation of the biological activity on osteosarcoma (OS) are non existent. In this report, we explored the chemical composition of French Polynesian Padina pavonica extract (EPP) by spectrophotometric assays (total phenolic, flavonoid and tannin content, and antioxidant activity) and by gas chromatography-mass spectrometry (GC-MS) analysis, and provided EPP lipid and sterols profiles. Several compounds with relevant biological activity were also identified that suggest interesting pharmacological and health-protecting effects for EPP. Moreover, we demonstrated that EPP presents good anti-proliferative and pro-apoptotic activities against two OS cell lines, SaOS-2 and MNNG, with different cancer-related phenotypes. Finally, our data suggest that EPP might target different properties associated with cancer development and aggressiveness.

The Quest for Phenolic Compounds from Macroalgae: A Review of Extraction and Identification Methodologies

“…Although unusual in the extraction of phenolic compounds due to the higher temperatures of the extraction involved, at least two studies have used the Soxhlet apparatus in the extraction of phenolic compounds from brown macroalgae, namely from the macroalga Sargassum wightii [56], from which some phlorotannins were identified after fractionation, and from Padina pavonica [92]. In this last study, the phenolic fraction was only characterized by spectrophotometric methods, namely, in terms of TPC, TFC, and total tannins content (TTC) (see Section 4.1).…”

“…The spectrophotometric characterization of the phenolic rich extracts from macroalgae involves mostly the estimation of TPC and TPhC, although some authors have also estimated the content of other specific families, such as flavonoids or anthocyanins [66,67,92,102,128]. The most commonly used method to determine the TPC in macroalgae extracts is the well-known Folin-Ciocalteu method, firstly established as Folin-Denis method [129] and then modified by Folin and Ciocalteu [130] and later by Singleton and Rossi [131].…”

“…Additionally, no consensus is found in the standard used for the calibration curves, which makes even more difficult the comparison between different studies. Most authors have expressed the TPC of macroalgae extracts as gallic acid [28,57,65,72,77,89,92,102,105,110,112,119,135] or phloroglucinol equivalents [104,136,137]. However, the use of other standards such as catechol [95,128] or tannic acid [54] has already been considered.…”

Macroalgal diversity for sustainable biotechnological development in French tropical overseas territories

“…Turbinaric acid, isolated from Turbinaria conoides from New Caledonia (Le Lann et al 2014) is a tris-nor-triterpene (C27), a derivative of squalene, which possesses cytotoxic properties (Kornprobst 2014). Studying brown seaweeds in French Polynesia, Bernardini et al (2018) highlighted interesting pharmacological and health-protecting effects, i.e. anti-proliferative and pro-apoptotic activities against two osteo-sarcoma cell lines, for phlorotannins isolated from Padina.…”

Alternative Treatments for Cancer Prevention and Cure [Part 1]

“…Seaweeds are specifically used to treat tumors in CHM [116]. Several studies revealed that active metabolites among the terpenoids, including carotenoids, polyphenols and alkaloids that can obtained from marine source [117][118][119][120][121][122][123][124][125]. Compounds from natural sources with antiproliferative activity represent an important and novel alternative to treat several types of cancer.…”