Pro-Adrenocorticotropin/Endorphin-Derived Peptides: Coordinate Action on Adrenal Steroidogenesis

Pedersen, Robert C.; Brownie, Alexander C.; Ling, Nicholas

doi:10.1126/science.6246578

Cited by 206 publications

(53 citation statements)

References 37 publications

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“…Furthermore, Page et al (32) demonstrated that the concomitant administration of a-MSH and growth hormone stimulated aldosterone secretion in hypophysectomized sodium-depleted rats, while Vinson et al (33) reported that a-MSH stimulated aldosterone production by adrenal glomerulosa cells in vitro. in hypophysectomized rats (34 tides may be involved in the regulation of aldosterone secretion. It has been shown that 13-LPH increases the level of plasma aldosterone in the rat in vivo (35).…”

Section: Resultsmentioning

confidence: 99%

Effects of β-Lipotropin and β-Lipotropin-derived Peptides on Aldosterone Production in the Rat Adrenal Gland

Matsuoka¹,

Mulrow²,

Franco‐Saenz³

et al. 1981

J. Clin. Invest.

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A B S T R A C T To investigate the role of non-ACTH pituitary peptides on steroidogenesis, we studied the effects of synthetic 3-lipotropin, (8-melanotropin, and p-endorphin on aldosterone and corticosterone stimulation using rat adrenal collagenase-dispersed capsular and decapsular cells. f8-lipotropin induced a significant aldosterone stimulation in a dose-dependent fashion (10 nM-1 ,uM). 8-endorphin, which is the carboxyterminal fragment of 8-lipotropin, did not stimulate aldosterone production at the doses used (3 nM-6 ,uM).

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Section: Resultsmentioning

confidence: 99%

Effects of β-Lipotropin and β-Lipotropin-derived Peptides on Aldosterone Production in the Rat Adrenal Gland

Matsuoka¹,

Mulrow²,

Franco‐Saenz³

et al. 1981

J. Clin. Invest.

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“…Recently, a trypsin digest of the N-terminal peptide of POMC, prepared from mouse pituitary tumor cells, and a bovine ? '3-melanotropin (MSH) synthetic peptide, representing a portion of the N-terminal peptide, have been shown to have a marked effect on the potentiation of adrenocortical response to ACTH (39,40). In another study, a naturally-occurring human pro-r-MSH glycopeptide potentiated the ACTH-induced steroidogenic response of isolated perfused rat and human adre nocortical cells (41).…”

Section: Discussionmentioning

confidence: 96%

Activities of ACTH-Potentiating Substances Isolated from Rat Serum on Steroidogenes s in Isolated Rat Adrenal Cells

Morita

1986

Japanese Journal of Pharmacology

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Abstract-Peptides that potentiate the response of adrenal cells to ACTH1__24 were isolated from rat serum.ACTH-potentiating activity was found in fractions of 9,000-40,000 in molecular weight (APS-Fr) and of smaller molecular weight (SM-Fr) on Sephadex G-100 gel filtration of the serum extract.The peptides were isolated from .APS-Fr by preparative acid polyacrylamide gel electrophoresis and named d1, d, d2, e, f and g. Their molecular weights, estimated by Sephadex G-75 gel filtration, were 41,000, 33,000, 24,000, 17,500, 17,500 and 16,000, respectively.All of these peptides were glycopeptides. The isoelectric point of peptide d was 8.45 and some of the others, such as f and g, were more basic. Sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis revealed that these peptides were decomposed into various fragments.ACTH-potentiating activity was highest in peptide d1 and lowest in peptide e. The maximum activity of peptide d was observed at 3x10-8 M when steroidogenesis was induced by 9 X 10-12 M ACTH,-24.While these peptides did not show any ACTH-like activity at any stage of isolation, the fractions of these peptides eluted from a Sephadex G-75 column showed more or less ACTH-like activity. These peptides therefore seemed to possess latent ACTH-like activity.The molecular weight of SM-Fr was smaller than ACTH1 _24. The ACTH-potentiating activity of SM-Fr was low, and SM-Fr did not show any ACTH-like activity.SM-Fr therefore seems to be the smallest structure which has ACTH-potentiating activity. The similarity of these peptides to proopiomelanocortin-related substances was discussed.

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“…The synthetic peptide y3-MSH, which represents a portion of the amino terminal or 16,000 fragment of pro-OLMC, has been shown to potentiate the stimulation of aldosterone and corticosterone production by ACTH in hypophysectomized rats (15) and isolated rat adrenocortical cells (16) but y3-MSH itself had no steroidogenic activity. In a subsequent study, ly3-MSH has been reported to stimulate aldosterone release from human adrenal adenoma cells in vitro (18).…”

Section: Resultsmentioning

confidence: 99%

“…Intravenous injection of a synthetic peptide representing a portion of the amino terminal or 16,000 fragment of pro-OLMC, termed y3-MSH, potentiated the stimulation of corticosterone and aldosterone by (1-24) ACTH in hypophysectomized rats (15). Furthermore, the amino-terminal region of pro-OLMC has been reported to potentiate slightly but significantly the action of (1-24) ACTH in isolated rat adrenocortical cells (16).…”

mentioning

confidence: 99%

Beta endorphin selectively stimulates aldosterone secretion in hypophysectomized, nephrectomized dogs.

Güllner¹,

Gill²

1983

J. Clin. Invest.

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A B S T R A C T We examined the effects of synthetic human /-endorphin (BEND) and a stable methionine (Met)-enkephalin analogue on aldosterone and cortisol secretion rates in anesthetized, hypophysectomized, and nephrectomized dogs and compared them to those of (1-39) ACTH. The circulation of the adrenal glands was completely isolated on the arterial and venous sides (Hilton Pouch). The peptides were infused to deliver 3 pmol/min into the aortic "pouch." Blood was collected from the vena caval pouch, which received blood only from the adrenal gland. Secretion rates of aldosterone and cortisol were calculated as the product of adrenal blood flow and venous steroid concentration. Duplicate steroid measurements were obtained during a control period, at 10, 30, and 50 min of peptide infusion and during a postcontrol period. BEND increased aldosterone secretion rate from 2.4±0.5 ng/ min (mean±SEM) to 3.2±0.9 ng/min at 10 min (N.S.), 8.2±2.5 ng/min (P < 0.05) at 30 min and 11.0±3.7 ng/ min (P < 0.05) at 50 min of infusion. Cortisol secretion rate was not affected by infusion of SEND. Infusion of the stable Met-enkephalin analogue D-alanine2; Metphenylalanine4, Met(O)-enkephalin-ol or saline alone had no effect on aldosterone or cortisol secretion rates. ACTH infusion increased mean aldosterone secretion rate by -215% and significantly stimulated cortisol secretion rate. These results indicate that BEND selectively stimulates aldosterone secretion with a potency similar to that of an equimolar dose of ACTH. INTRODUCTION There is considerable indirect evidence suggesting that an unidentified pituitary factor other than ACTH

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Pro-Adrenocorticotropin/Endorphin-Derived Peptides: Coordinate Action on Adrenal Steroidogenesis

Cited by 206 publications

References 37 publications

Effects of β-Lipotropin and β-Lipotropin-derived Peptides on Aldosterone Production in the Rat Adrenal Gland

Effects of β-Lipotropin and β-Lipotropin-derived Peptides on Aldosterone Production in the Rat Adrenal Gland

Activities of ACTH-Potentiating Substances Isolated from Rat Serum on Steroidogenes s in Isolated Rat Adrenal Cells

Beta endorphin selectively stimulates aldosterone secretion in hypophysectomized, nephrectomized dogs.

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