Pristimerin effectively inhibits the malignant phenotypes of uveal melanoma cells by targeting NF-κB pathway

Zhang, Biao; Zhang, Jing; Pan, Jingxuan

doi:10.3892/ijo.2017.4079

Cited by 23 publications

(38 citation statements)

References 43 publications

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“…Also, NF-κB pathway has been implicated in the pathogenesis of malignant melanoma. It was reported some medicines realized the melanoma-inhibiting effect through inactivating NF-κB pathway [24,44]. Previous studies have shown that activation of NF-κB can inhibit apoptosis induced by a number of stimuli [45].…”

Section: Discussionmentioning

confidence: 99%

MicroRNA-29a Inhibits Growth, Migration and Invasion of Melanoma A375 Cells in Vitro by Directly Targeting BMI1

Xiong

Liu

Qiu

et al. 2018

Cell Physiol Biochem

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Background/Aims: Melanoma is one of the most aggressive malignant tumors, with increasing incidence, poor prognosis, and lack of any effective targeted therapies. Abnormal expression of miR-29a has been found in several types of cancers, including melanoma. In this study, experiments were performed to investigate the role of miR-29a in melanoma, and the molecular mechanism by which miR-29a represses melanoma. Methods: miR-29 and Bmi1 expression was examined by quantitative real-time polymerase chain reaction (qRT-PCR). The cell viability, apoptosis, migration and invasion were respectively determined by Cell Counting Kit-8 assay, Propidium iodide (PI) fluorescein isothiocynate (FITC)-Annexin V staining assay, wound healing assay and transwell assay. Luciferase reporter assay was performed to determine a target gene of miR-29a. Western blot was used to analyze protein expression of apoptosis-related proteins, Bmi1, Wnt/β-catenin and Nuclear factor-κB (NF-κB) pathway target genes. Results: miR-29a was down-regulated in all tested melanoma cell lines. Up-regulation of miR-29a effectively inhibited cell viability, migration, and invasion, but promoted apoptosis in A375 cells. Bmi1 was a direct target gene of miR-29a. Transfection with miR-29a mimic decreased cell migration and invasion and Bmi1 expression in Malme-3M cells, SK-MEL-2, SK-MEL-5, and M14 cell lines. Moreover, miR-29a might suppress growth, migration and invasion of A375 cells by negatively regulating Bmi1. In addition, our results demonstrated that transfection with miR-29a mimic effectively blocked Wnt/β-catenin and NF-κB pathways via down-regulating Bmi1. Conclusion: miR-29a could be functioned as a potential tumor suppressor through direct regulation of Bmi1 in melanoma cells.

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Section: Discussionmentioning

confidence: 99%

MicroRNA-29a Inhibits Growth, Migration and Invasion of Melanoma A375 Cells in Vitro by Directly Targeting BMI1

Xiong

Liu

Qiu

et al. 2018

Cell Physiol Biochem

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“…18 2.8 | Cell cycle analysis by flow cytometry EC9706 and EC109 cells were seeded in 6-well plates and treated with or without pristimerin for 48 hours. 18 2.8 | Cell cycle analysis by flow cytometry EC9706 and EC109 cells were seeded in 6-well plates and treated with or without pristimerin for 48 hours.…”

Section: Colony Formation Assaymentioning

confidence: 99%

“…18,25 EC9706 and EC109 cells were incubated with (2.0 μM) or without pristimerin for 24 hours, and the ALDH + cells were detected by flow cytometry. 18,25 EC9706 and EC109 cells were incubated with (2.0 μM) or without pristimerin for 24 hours, and the ALDH + cells were detected by flow cytometry.…”

Section: Pristimerin Attenuates the Properties Of Cancer Stem-like mentioning

confidence: 99%

“…13 NF-κB inhibition by Bay11-7082 can suppress tumour growth, angiogenesis, and metastasis of human EC. [16][17][18] Consequently, pristimerin possessed anti-proliferation, apoptosis-inducing, and antiinflammatory activities. 15 Pristimerin, a natural quinonemethide triterpenoid compound isolated from traditional Chinese herbals, demonstrated to inhibit the activity of NF-κB pathway in chronic myelogenous leukaemia, acute myelogenous leukaemia, and uveal melanoma.…”

Section: Introductionmentioning

confidence: 99%

“…14 Therefore, NF-κB pathway may represent a valuable therapeutic target in the treatment of EC. [18][19][20] Furthermore, pristimerin suppresses metastasis of prostate cancer by targeting CSCs, 21 which are responsible for chemo-resistance, metastasis, and recurrence. [16][17][18] Consequently, pristimerin possessed anti-proliferation, apoptosis-inducing, and antiinflammatory activities.…”

Section: Introductionmentioning

confidence: 99%

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Pristimerin targeting NF‐κB pathway inhibits proliferation, migration, and invasion in esophageal squamous cell carcinoma cells

Tan

Zhou

et al. 2018

Cell Biochemistry & Function

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Although several approaches including surgery, chemotherapy, and radiotherapy had been applied in the treatment of ESCC, more effective targeted chemotherapies are required to increase the survival rates of patients. This study suggested that inhibiting NF-κB signalling pathway could be an effective approach for the treatment of ESCC. Pristimerin, a potent NF-κB inhibitor, exerted potent anti-ESCC effects both in vitro and in vivo, which may be a promising therapeutic agent for ESCC.

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Identification of differentially expressed genes and functional annotations associated with metastases of the uveal melanoma

Han

Wang

et al. 2019

J of Cellular Biochemistry

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Uveal melanoma (UVM) is an adult intraocular malignancy which is the most frequent and has a high tendency for metastasis. This study aims to develop significant differential gene subnetwork between primary and metastatic UVM to identify potential prognostic biomarkers. Differentially expressed genes (DEGs) among three chip datasets were downloaded from Gene Expression Omnibus and identified according to standardization annotation information. Genetic enrichment analyses were utilized to describe biologic functions. The protein‐protein interaction network of DEGs was developed and the module analysis was constructed by STRING and Cytoscape. Kaplan‐Meier method of the integrated expression score was applied to analyze survival outcomes. Functional annotation was assessed to perform GO and Kyoto Encyclopedia of Genes and Genomes enrichment analysis. In addition, ClueGO and gene set enrichment analysis were analyzed to detect underlying significant genes and involved signaling pathways. A total of 103 DEGs with function enrichment were recognized and might be considered as candidate prognostic biomarkers between primary and metastatic UVM. Furthermore, Kaplan‐Meier method suggested that SCD5, SPTBN1, FABP5, SQLE, PTPLA (HACD1), and CDC25B were independent prognostic factors in UVM. Functional annotations indicated that the most involved significant pathways including interferon‐gamma response, IL‐6 JAK STAT3 signaling, TNFA signaling via NFKB and inflammatory response. Significant DEGs between primary and metastatic UVM tissue were identified and might have involved in the metastasis of UVM. SCD5, SPTBN1, FABP5, SQLE, PTPLA (HACD1), and CDC25B transcription levels were of high prognostic value, which might assist us to understand the underlying carcinogenesis or advancement of UVM better.

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Pristimerin effectively inhibits the malignant phenotypes of uveal melanoma cells by targeting NF-κB pathway

Cited by 23 publications

References 43 publications

MicroRNA-29a Inhibits Growth, Migration and Invasion of Melanoma A375 Cells in Vitro by Directly Targeting BMI1

MicroRNA-29a Inhibits Growth, Migration and Invasion of Melanoma A375 Cells in Vitro by Directly Targeting BMI1

Pristimerin targeting NF‐κB pathway inhibits proliferation, migration, and invasion in esophageal squamous cell carcinoma cells

Identification of differentially expressed genes and functional annotations associated with metastases of the uveal melanoma

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