“…The use of patient fat cells (adipocytes) to print customized tissue configurations such as breast reconstruction has also been studied (Melchelo et al 2011). Yanez et al (2012) have recently investigated a 3-layer, 3D-printed skin grafts by printing the dermal layer onto a biopaper (biomimetic hydrogel) using a collagen bioink (hydrogel matrix) with fibroblast cells, an intermediate layer using a fibrin gel bioink containing endothelial cells, and an outer or epidermal layer using a collagen-based hydrogel bioink containing keratinocyte cells. The strategy in this approach was to encourage vascularization in the intermediate layer printed with endothelial cells (Fig.…”