Principles Underlying Cryopreservation and Freeze-Drying of Cells and Tissues

Wolkers, Willem F.; Oldenhof, Harriëtte

doi:10.1007/978-1-0716-0783-1_1

Cited by 47 publications

(50 citation statements)

References 107 publications

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“…Moreover, PRP, FCS and combinations of the different HAS formulations in the presence of HA were also compared. It is widely known that lyophilization, or freeze-drying, of sterile therapeutics extends the long-term stability of molecules, including large molecules such as proteins [ 34 , 35 ]. For this reason, lyophilization is currently involved in the manufacturing process of 50% of the total biopharmaceutical products [ 36 , 37 ].…”

Section: Discussionmentioning

confidence: 99%

Optimization of Lyophilized Hyperacute Serum (HAS) as a Regenerative Therapeutic in Osteoarthritis

Calvo¹,

Kuten²,

Kramer

et al. 2021

IJMS

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Hyperacute serum (HAS) is a blood derivative product that promotes the proliferation of various cell types and controls inflammation in vitro. The aim of this study is to investigate the regenerative potential of different formulations of HAS, including lyophilized and hyaluronic acid combined versions, to obtain a stable and standardized therapeutic in osteoarthritis (OA), which may be able to overcome the variability limitations of platelet-rich plasma (PRP). Primary human osteoarthritic chondrocytes were used for testing cellular viability and gene expression of OA-related genes. Moreover, a co-culture of human explants of cartilage, bone and synovium under inflammatory conditions was used for investigating the inflammatory control capacities of the different therapeutics. In this study, one formulation of lyophilized HAS achieved the high cell viability rates of liquid HAS and PRP. Gene expression analysis showed that HAS induced higher Col1a1 expression than PRP. Cytokine quantification from supernatant fluids revealed that HAS treatment of inflamed co-cultures significantly reduced levels of IL-5, IL-15, IL-2, TNFα, IL-7 and IL-12. To conclude, lyophilized HAS is a stable and standardized therapeutic with high potential in joint regeneration.

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Section: Discussionmentioning

confidence: 99%

Optimization of Lyophilized Hyperacute Serum (HAS) as a Regenerative Therapeutic in Osteoarthritis

Calvo¹,

Kuten²,

Kramer

et al. 2021

IJMS

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“…The cryopreservation of the gametes of females is challenged by the small number of cells, differences in developmental stages, and the invasive procedures needed to access the oocytes or embryos. Embryo cryopreservation has reached a commercial level only in the cattle industry (Saragusty 2012), whereas cryopreserved sperm is almost used entirely for artificial insemination (Wolkers and Oldenhof 2021). In general, only a few biobanks holding biomaterials from domesticated animals exist and are hosted by veterinary hospitals, zoological gardens, breeding and diagnostics companies, national farm animal genetic resource gene banks, research institutes, and universities (Groeneveld et al 2016).…”

Section: Animal Biomaterialsmentioning

confidence: 99%

“…In animal biomaterials, additional contaminations must be considered when tissues and organs are not obtained under sterile conditions. Thereby, semen of most domestic species such as a stallion is typically collected for cryopreservation outside the breeding station (Wolkers and Oldenhof 2021). In addition, the collection method can have profound effects on the microbial load of sample materials.…”

Section: Sampling and Tank Environmentmentioning

confidence: 99%

Microbial occurrence in liquid nitrogen storage tanks: a challenge for cryobanking?

Bajerski

Nagel

Overmann

2021

Appl Microbiol Biotechnol

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Modern biobanks maintain valuable living materials for medical diagnostics, reproduction medicine, and conservation purposes. To guarantee high quality during long-term storage and to avoid metabolic activities, cryostorage is often conducted in the N2 vapour phase or in liquid nitrogen (LN) at temperatures below − 150 °C. One potential risk of cryostorage is microbial cross contamination in the LN storage tanks. The current review summarises data on the occurrence of microorganisms that may compromise the safety and quality of biological materials during long-term storage. We assess the potential for the microbial contamination of LN in storage tanks holding different biological materials based on the detection by culture-based and molecular approaches. The samples themselves, the LN, the human microbiome, and the surrounding environment are possible routes of contamination and can cause cross contaminations via the LN phase. In general, the results showed that LN is typically not the source of major contaminations and only a few studies provided evidence for a risk of microbial cross contamination. So far, culture-based and culture-independent techniques detected only low amounts of microbial cells, indicating that cross contamination may occur at a very low frequency. To further minimise the potential risk of microbial cross contaminations, we recommend reducing the formation of ice crystals in cryotanks that can entrap environmental microorganisms and using sealed or second sample packing. A short survey demonstrated the awareness for microbial contaminations of storage containers among different culture collections. Although most participants consider the risk of cross contaminations in LN storage tanks as low, they prevent potential contaminations by using sealed devices and − 150 °C freezers. It is concluded that the overall risk for cross contaminations in biobanks is relatively low when following standard operating procedures (SOPs). We evaluated the potential sources in detail and summarised our results in a risk assessment spreadsheet which can be used for the quality management of biobanks. Key points • Identification of potential contaminants and their sources in LN storage tanks. • Recommendations to reduce this risk of LN storage tank contamination. • Development of a risk assessment spreadsheet to support quality management.

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“…The survival of plant cells after freezing is dependent on the lethal effects of intracellular ice. Ice crystals affect the organization of the cytoplasm and damage the membranes of cell organs by increasing their volume (Wolkers and Oldenhof 2021). The formation of intracellular ice can be effectively minimized by the cooling rate (Mazur 2004).…”

Section: Fast Cooling Lowers the Extent And/or Amount Of Ice Crystal Induced Damagementioning

confidence: 99%

“…At very slow cooling rates (~0.1 to 5 °C min -1 ), ice is formed intercellularly. The chemical potential between cells inside and outside leads to water efflux, cell dehydration (Wolkers and Oldenhof 2021) and eventually vitrification of the cytoplasm. However, the dehydration during slow cooling may severely damage desiccation-sensitive cells (Wesley- .…”

Section: Fast Cooling Lowers the Extent And/or Amount Of Ice Crystal Induced Damagementioning

confidence: 99%

Fast Drying Combined With Rapid Cooling Can Enhance The Survival of The Desiccation Sensitive Wheat Pollen After Ultra-Low Temperature Storage

Impe

Ballesteros

Nagel

2021

Preprint

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Long-term storage of pollen is important for the fertilization of spatially or temporally isolated female parents, especially during hybrid breeding. Wheat pollen is dehydration-sensitive and rapidly loses viability after shedding. To preserve wheat pollen, we hypothesized that fast-(flash)-drying and fast cooling (150°C min-1) compared to slow-(air)-drying and slow cooling (1°C min-1) would increase the rate of intracellular water content (WC) removal, decrease intracellular ice crystal formation, and increase viability after exposure to ultra-low temperatures. High correlations were found between pollen WC and viability analyzed by impedance flow cytometry (IFC viability: r=0.92, P<0.001) and pollen germination (r=0.94, P<0.001). After 10 min of air-drying, 66% WC was lost and pollen germination was at 12.2±12.3%. After 10 min of flash-drying, WC of pollen reduced by 74%. IFC viability decreased from 90.2±6.7 to 39.4±17.9%, and pollen germination dropped from 33.7±16.9 to 1.9±3.9%. After 12 min of flash-drying, WCs decreased to <0.34 mg H2O mg-1 DW, ice crystal formation was completely prevented (ΔH=0 J mg-1 DW), and pollen germination reached 1.2±1.0%. After slow and fast cooling, flash-dried pollen (WC 0.91±0.11 mg H2O mg-1 DW) showed less ice crystal formation during cryomicroscopic-video-recordings and had IFC viability of 4.5±7.0% (slow) and 6.1±8.8% (fast), respectively, compared to air-dried pollen which lost all viability. Generally, fast-(flash)-drying and increased cooling rates may enable the survival of wheat pollen likely due to (1) a fast rate of intracellular WC loss that reduces deleterious biochemical changes associated with the drying process and (2) a delay and reduction in intracellular ice crystal formation.

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Principles Underlying Cryopreservation and Freeze-Drying of Cells and Tissues

Cited by 47 publications

References 107 publications

Optimization of Lyophilized Hyperacute Serum (HAS) as a Regenerative Therapeutic in Osteoarthritis

Optimization of Lyophilized Hyperacute Serum (HAS) as a Regenerative Therapeutic in Osteoarthritis

Microbial occurrence in liquid nitrogen storage tanks: a challenge for cryobanking?

Fast Drying Combined With Rapid Cooling Can Enhance The Survival of The Desiccation Sensitive Wheat Pollen After Ultra-Low Temperature Storage

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