Principles of Isolation, Cultivation, and Conservation of Bacteria

Stolp, H.; Starr, Mortimer P.

doi:10.1007/978-3-662-13187-9_5

Cited by 12 publications

(5 citation statements)

References 136 publications

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“…Most bacteria and fungi can be grown in culture under appropriate conditions (Stolp and Starr, 1981;Fahy and Hayward, 1983;Hayward. 1983;Dhingra and Sinclair, 1985;.…”

Section: Selective Mediamentioning

confidence: 99%

New Approaches to the Detection of Microbial Plant Pathogens

Chu

Waterhouse

Martín

et al. 1989

Biotechnology and Genetic Engineering Reviews

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“…Most bacteria and fungi can be grown in culture under appropriate conditions (Stolp and Starr, 1981;Fahy and Hayward, 1983;Hayward. 1983;Dhingra and Sinclair, 1985;.…”

Section: Selective Mediamentioning

confidence: 99%

New Approaches to the Detection of Microbial Plant Pathogens

Chu

Waterhouse

Martín

et al. 1989

Biotechnology and Genetic Engineering Reviews

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“…Most microorganisms are neutrophiles since they survive only within the pH range 5 to 8. 5 and exhibit maximum growth rates at close to neutrality (127). Gram-positive neutrophiles are particularly sensitive to extremes of pH (91).…”

Section: Microorganisms In Acidic Environmentsmentioning

confidence: 99%

“…These environments and their flora have been reviewed by Langworthy (76). Many of the bacterial species which grow in acidic lakes and soils (pH 3.5 to 5.0) also grow well at neutral pH, and many of the enrichment media used in the isolation of these organisms are buffered at close to neutrality (127). Such organisms are therefore best described as acid tolerant and include species of the following genera: Plantomyces, Bactoderma, Caulobacter, and Microcyclus (56,120).…”

Section: Microorganisms In Acidic Environmentsmentioning

confidence: 99%

Energy conservation in acidophilic bacteria.

Cobley¹,

Cox²

1983

Microbiological Reviews

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“…Above pH 10.0, the lipase production was suppressed and ceased at pH 12.0. Most microorganisms can survive within a pH range 5 to 8.5 and exhibit maximum growth rates at close to neutrality 33 . In the case of Aeromonas sp., growth and lipase secretion were highest at neutral to alkaline pH 34 .…”

Section: Effect Of Carbon Sources and Physical Parameters On Lipase Pmentioning

confidence: 99%

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Charoenpanich¹,

Suktanarag²,

Toobbucha³

2011

ScienceAsia

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Aeromonas sp. EBB-1 isolated from marine sludge in Angsila, Thailand produced an extracellular thermophilic lipase. Maximum lipase activity of the culture medium was obtained after 15 h at 25°C and pH 8.0 with 0.5% (v/v) olive oil as a carbon source. The enzyme was successfully purified 30-fold to homogeneity by successive ammonium sulphate precipitation, gel-permeation column chromatography, and ultrafiltration. The purified bacterial lipase possessed a relative molecular mass of 45 kDa. Highest lipase activity was determined at pH 8.0 and 37°C with p-nitrophenyl palmitate as a substrate. The enzyme was stable at pH 6.0-8.0 and at temperatures of 30-80°C. Enzyme activity decreased slightly in 50% (v/v) isoamyl alcohol, decane, or heptane. Lipase activity decreased to a half in the presence of butanol and benzene, and by more than 60% in DMSO, methanol, ethanol, and hexadecane. Lipase activity was inhibited by most of the salt ions (except Ca 2+ ions) as well as by Tween 80, DTT, PMSF, SDS, and EDTA. Although lipase showed variable specificity/hydrolytic activity towards a number of p-nitrophenyl esters, it was preferentially active towards long-carbon chain acyl esters (C12-C16). The high temperature stability and ability to hydrolyse long length esters support the potential of this lipase enzyme as a vigorous biocatalyst for industrial applications.

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Principles of Isolation, Cultivation, and Conservation of Bacteria

Cited by 12 publications

References 136 publications

New Approaches to the Detection of Microbial Plant Pathogens

New Approaches to the Detection of Microbial Plant Pathogens

Energy conservation in acidophilic bacteria.

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