Principles of Immunophenotyping

Naeim, Faramarz; Rao, P. Nagesh; Song, Sophie X.; Phan, Ryan T.

doi:10.1016/b978-0-12-809843-1.00002-4

Cited by 38 publications

(44 citation statements)

References 7 publications

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“…1a, middle). As CD14 can be expressed in other immune cells, we then used a second marker, CD68, to specifically identify the peritoneal monocyte and macrophage populations [27,29]. The expression level of CD14 and CD68 was correlated, with contour plots revealing distinct CD14 +low /CD68 +low and CD14 +high /CD68 +high subpopulations in both women with and without endometriosis (Fig.…”

Section: Peritoneal Macrophages Are Divided Into Cd14 +Low /Cd68 +Lowmentioning

confidence: 99%

Endometriosis Patients Show an Increased M2 Response in the Peritoneal CD14+low/CD68+low Macrophage Subpopulation Coupled with an Increase in the T-helper 2 and T-regulatory Cells

et al. 2020

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Endometriosis is a chronic inflammatory disease associated with an impaired immune response at the site of lesion implantation. The ability of macrophages to respond to changes in their environment is critical for an effective immune response. However, the existing knowledge of the peritoneal immune cell populations, their activation state and contribution to the immunological changes that occur in endometriosis are still controversial and inconclusive. In this study, we have examined the relative abundance of peritoneal macrophage subtypes, in women with (n = 21) versus without (n = 18) endometriosis and diseaseassociated changes in the adaptive T cell response. Using flow cytometry, we showed that peritoneal fluid monocyte/ macrophages are composed of two populations of cells that exhibit major differences in the levels of the CD14 and CD68 markers, which we classified as the CD14 +low /CD68 +low and CD14 +high /CD68 +high subpopulations. Moreover, endometriosisassociated changes in the macrophage subtypes occurred only in the CD14 +low /CD68 +low subpopulation. In this subpopulation, we found an increased macrophage type 2 response that was coupled with an increase in peritoneal T-helper 2 and T-regulatory cell populations in women with endometriosis, compared with controls. In summary, this study resolves conflicting data in the literature regarding changes in the peritoneal immune cell population in endometriosis and identifies CD14 +low /CD68 +low macrophages as the subpopulation that changes in response to the disease.

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Section: Peritoneal Macrophages Are Divided Into Cd14 +Low /Cd68 +Lowmentioning

confidence: 99%

Endometriosis Patients Show an Increased M2 Response in the Peritoneal CD14+low/CD68+low Macrophage Subpopulation Coupled with an Increase in the T-helper 2 and T-regulatory Cells

et al. 2020

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“…NK cells may also have cytoplasmic expression of CD3. Nonspecific cytoplasmic staining of plasma cells and macrophages is also possible [18].…”

Section: Discussionmentioning

confidence: 99%

Immunohistochemical Characterisation of Lymphoid Populations in Rabbit Jejunal Peyer’s Patch

Khomych¹,

Fedorenko²

2020

EUREKA: Life Sciences

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Rabbits are an important livestock animal species, which are used for their meat and fur. Nowadays they are also becoming more popular as pets. Furthermore, rabbits are commonly used in research, inter alia in immunological studies and for studying pathogenesis of human and animal diseases. The lymphoid tissue is abundant in the rabbit intestine and a lot of it is concentrated in Peyer's patches, the majority of which is located in the jejunum. Understanding of the rabbit Peyer's patches functions is essential for the prevention and treatment of their diseases. In order to enhance it, accurate knowledge of its lymphocyte population composition is needed. At present, the cellular composition of the rabbit gut-associated lymphoid tissue remains insufficiently studied. Therefore, the aim of our study was to establish the content and localization of lymphoid populations in the domestic rabbit jejunal Peyer’s patches. We performed immunohistochemical studies using monoclonal antibodies CD3, CD10 and CD20 (DAKO, Denmark) to identify the distribution of lymphoid populations in jejunal Peyer's patches of a mature domestic rabbit. The results, obtained in our study, complement the existing data and determine the main lymphoid populations in the rabbit jejunal Peyer's patches. The presented data are fundamental for further studies of the rabbit gut-associated lymphoid tissue. We found that lymphoid nodules of jejunal Peyer's patches contains a predominant CD20+ cell population (B cells). It also contains many CD10+ cells (precursors of T cells and B cells), which are mostly concentrated in the germinal centers of lymphoid nodules. In the diffuse form of the lymphoid tissue the vast majority of cells are CD3+ cells (T cells)

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“…We used primary human T lymphocytes in our in vitro human leukocytes model to avoid any proteolytic effect on human endocan, as it can be proteolytically cleaved by neutrophils 10 . This choice was justified by the expression of LFA‐1 in all leukocyte subsets, and especially known to be found at the surface of T lymphocytes and neutrophils 12 . Additionally, blocking of LFA‐1 has been shown to inhibit diapedesis in both neutrophils 13–15 and T lymphocytes 16–18 because of the critical role of LFA‐1 in the firm adhesion step, which is mandatory for transmigration 19 .…”

Section: Methodsmentioning

confidence: 99%

Cleaved endocan acts as a biologic competitor of endocan in the control of ICAM-1-dependent leukocyte diapedesis

Gaudet

Portier

Mathieu

et al. 2020

Journal of Leukocyte Biology

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Dysregulated leukocyte diapedesis is a major contributor to acute severe inflammatory states like sepsis and acute respiratory distress syndrome, which are common conditions in critically ill subjects. Endocan is a circulating proteoglycan that binds to the leukocyte integrin LFA-1 and blocks its interaction with its endothelial ligand ICAM-1, subsequently leading to the inhibition of leukocyte recruitment. Recent data have highlighted the hypothetic role of p14, endocan's major catabolite found in the bloodstream of septic patients, as a potential antagonist of endocan, thus participating in the regulation of acute inflammation. We hereby characterize the role of p14 as a biologic competitor of endocan, through assessment of its molecular interactions with LFA-1, endocan, and ICAM-1, as well as its effects on human leukocyte trafficking. Using immunodetection assay, we report that p14 can bind to LFA-1, thus inhibiting the interaction between LFA-1 and endocan, which in turn leads to the restoration of the ICAM-1/LFA-1 interaction. In primary human T cells trafficking assays, we underline the absence of effect of p14 on ICAM-1-dependent adhesion and migration, as well as on transendothelial migration. However, in those models, p14 reverses the antimigratory effect of endocan. To conclude, our study supports the hypothesis of an antagonistic role of p14 versus endocan in its effect on the LFA-1/ICAM-1-dependent human leukocyte recruitment. K E Y W O R D Sacute lung injury, cleaved endocan, endocan, inflammation, leukocyte diapedesis, p14

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Principles of Immunophenotyping

Cited by 38 publications

References 7 publications

Endometriosis Patients Show an Increased M2 Response in the Peritoneal CD14+low/CD68+low Macrophage Subpopulation Coupled with an Increase in the T-helper 2 and T-regulatory Cells

Endometriosis Patients Show an Increased M2 Response in the Peritoneal CD14+low/CD68+low Macrophage Subpopulation Coupled with an Increase in the T-helper 2 and T-regulatory Cells

Immunohistochemical Characterisation of Lymphoid Populations in Rabbit Jejunal Peyer’s Patch

Cleaved endocan acts as a biologic competitor of endocan in the control of ICAM-1-dependent leukocyte diapedesis

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