Principles of Enzymic Histo‐ and Cytochemistry

Glick, David

doi:10.1002/9780470122549.ch10

Cited by 21 publications

(18 citation statements)

References 103 publications

(5 reference statements)

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“…It was estimated with reference to the calibration curve and expressed as µg g −1 tissue FW. The total phenol content was determined by Malick and Singh (1980) and the concentration of phenols express as mg phenols/1 g extract. Estimation of Sucrose - metabolizing enzymes: Sucrose phosphate synthase (SPS) and sucrose synthase (SS) activity were estimated by the method described by Hubbard et al (1989).…”

Section: Methodsmentioning

confidence: 99%

Adaptive physiological and biochemical response of sugarcane genotypes to high-temperature stress

Kohila

Gomathi

2018

Ind J Plant Physiol.

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Impact of elevated temperature on physiological and biochemical changes were evaluated in 5 commercial sugarcane genotypes and 2 wild species clones at two different growth phases. The study revealed that heat stress decreased chlorophyll content, chlorophyll stability index (CSI), SPAD value, maximum quantum efficiency of PSII photochemistry (Fv/Fm ratio), leaf gas exchange parameters, relative water content (RWC), and activities of nitrate reductase (NR), sucrose-metabolizing enzymes (SPS, SS, AI, NI) in all the genotypes and species clones. In contrast, elevated temperature induced an increase in proline, total phenolics content (TP), antioxidant enzyme activities (SOD and POX), lipid peroxidation (LP), membrane injury index (MII) and soluble sugar content in all clones. Principal component analysis based on physiological heat tolerance indexes could clearly distinguish sugarcane genotypes into three heat tolerance clusters. Noteworthy in comparison to the heat-sensitive varieties, sugarcane genotype that possessed higher degrees of heat tolerance Co 99004 displayed higher chlorophyll content, CSI, antioxidant enzyme activities, NR activity, RWC, total phenols, sucrose-metabolizing enzymes, soluble sugar content and leaf gas exchange and lower level of lipid peroxidation and membrane injury index.

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Section: Methodsmentioning

confidence: 99%

Adaptive physiological and biochemical response of sugarcane genotypes to high-temperature stress

Kohila

Gomathi

2018

Ind J Plant Physiol.

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“…Total phenolic content was determined by Folin-Ciocalteu reagent using the method of Malick and Singh [46] and expressed as mg gallic acid equivalent (GAE) g −1 of extract (mg g −1 ). The method of Zhishen et al [47] was employed for the estimation of flavonoid content.…”

Section: Total Phenols and Flavonoidsmentioning

confidence: 99%

Combined Kinetin and Spermidine Treatments Ameliorate Growth and Photosynthetic Inhibition in Vigna angularis by Up-Regulating Antioxidant and Nitrogen Metabolism under Cadmium Stress

Ahanger

Aziz

Sahli³

et al. 2020

Biomolecules

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Pot experiments were conducted to investigate the probable beneficial role of the individual as well as combined application of kinetin (50 μM Kn) and spermidine (200 μM Spd) on Vigna angularis under cadmium (Cd) stress. Cd treatment reduced growth by declining the content of chlorophylls and carotenoids, photosynthesis, and gas exchange parameters. Exogenously, Kn and Spd application enhanced the photosynthetic parameters and up-regulated the antioxidant system by improving the activities of antioxidant enzymes and the content of non-enzymatic components. In addition, the application of Kn and Spd resulted in significant improvement in the content of sugars, proline, and glycine betaine, ameliorating the decline in relative water content. Oxidative stress parameters including hydrogen peroxide, superoxide, lipid peroxidation, lipoxygenase activity, and electrolyte leakage increased due to Cd stress; however, the application of Kn and Spd imparted a significant decline in all these parameters. Further, reduced Cd uptake was also observed due to Kn and Spd application. Total phenols and flavonoids also increased due to Kn and Spd treatments under normal as well as Cd stress conditions, which may have further helped with the elimination of reactive oxygen species. Reduction in the activity of nitrate reductase and the content of nitrogen was ameliorated due to the exogenous application of Kn and Spd. Therefore, the exogenous application of Kn and Spd benefited Vigna angularis counteracting the damaging effects of Cd stress by up-regulating the tolerance mechanisms, including antioxidant and osmolyte metabolism.

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“…This curve was subsequently employed to quantify the protein content, with the outcomes being reported in milligrams per gram of dry weight. The estimation of total phenols was conducted using the method developed by Malick and Singh (Malick & Singh, 1980). The quantification of total phenols was conducted by employing a standard curve that was constructed by utilizing various quantities of gallic acid.…”

Section: Methodsmentioning

confidence: 99%

Aquaponics as a climate‐smart technology for sustainable food production: A comparison with conventional production system in United Arab Emirates

Nishanth,

Alshamsi,

Alkaabi

et al. 2024

J World Aquaculture Soc

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This research aimed to standardize the growth of two commonly cultivated herbs in the United Arab Emirates, basil (Ocimum basilicum L.) and coriander (Coriandrum sativum L.), within an aquaponic system. The study compared a recirculatory aquaponic system (RAS) with static aquaculture systems (for fish performance) and irrigated greenhouse cultivation systems (for herb performance). The project assessed growth, element composition, and aquaponic efficacy to determine the viability of aquaponics for these herbs. In the soilless (aquaponic) system, both herbs exhibited significantly enhanced growth parameters, including plant height, root length, leaf count, and fresh and dry weights, compared to conventional soil‐based cultivation. This growth enhancement was attributed to the additional nutrients derived from fish waste in the aquaponic system. Furthermore, plants displayed significantly higher element levels, highlighting the effectiveness of aquaponics in nutrient delivery. Notably, the aquaponic system demonstrated superior antioxidant activity in both herbs. The study also revealed remarkable water use efficiency in the aquaponic system, with water savings exceeding 90% compared to conventional methods. These findings establish the efficiency and sustainability of aquaponic systems over traditional food production approaches, particularly in arid regions such as the United Arab Emirates. Utilizing an aquaponic system for these commonly cultivated herbs resulted in improved growth and resource efficiency, affirming its viability as an environmentally sustainable and efficient method of food production in desert climates.

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Principles of Enzymic Histo‐ and Cytochemistry

Cited by 21 publications

References 103 publications

Adaptive physiological and biochemical response of sugarcane genotypes to high-temperature stress

Adaptive physiological and biochemical response of sugarcane genotypes to high-temperature stress

Combined Kinetin and Spermidine Treatments Ameliorate Growth and Photosynthetic Inhibition in Vigna angularis by Up-Regulating Antioxidant and Nitrogen Metabolism under Cadmium Stress

Aquaponics as a climate‐smart technology for sustainable food production: A comparison with conventional production system in United Arab Emirates

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