Principles of DNA separation with capillary electrophoresis

Heller, Christoph

doi:10.1002/1522-2683(200102)22:4<629::aid-elps629>3.0.co;2-s

Cited by 195 publications

(129 citation statements)

References 90 publications

(75 reference statements)

Supporting

Mentioning

126

Contrasting

Unclassified

Order By: Relevance

“…Provides a good guide for data analysis. 11) Principles of DNA separation with capillary electrophoresis [195]. Heller has published several seminal papers in this field.…”

Section: Discussion: An Annotated List Of Useful Articlesmentioning

confidence: 99%

Theory of DNA electrophoresis (∼ 1999 –2002 ½)

et al. 2002

View full text Add to dashboard Cite

Over the last two decades, the introduction of new methods such as pulsed-field gel electrophoresis and capillary array electrophoresis has made it possible to map and sequence entire genomes, including our own. The development of these experimental methods has been helped by the progress of theoretical and computational sciences, and the interactions between these three modi operandi of modern science are still pushing the limits of our technologies. We now see a clear trend towards proteomics and microfluidic (even nanofluidic!) devices. In this review, we take a look at the progress of the field over the last 3 years using the glasses of the theoretical scientist and focusing mostly on new ideas and concepts. About a dozen different subfields are discussed and reviewed. We conclude by giving a commented list of some of the best review articles published over the last 2-3 years.

show abstract

“…Provides a good guide for data analysis. 11) Principles of DNA separation with capillary electrophoresis [195]. Heller has published several seminal papers in this field.…”

Section: Discussion: An Annotated List Of Useful Articlesmentioning

confidence: 99%

Theory of DNA electrophoresis (∼ 1999 –2002 ½)

et al. 2002

View full text Add to dashboard Cite

show abstract

“…4 These polymers are widely used in capillary electrophoresis to improve the resolution in the separation of proteins 5 and DNAs. 6 It determines a specific region where the fluid viscosity and its shear rate has a linear relationship (on the usual log-log coordinates). Thus, the Carreau model predicts a power law region.…”

Section: Introductionmentioning

confidence: 99%

Boundary layer flow and heat transfer to Carreau fluid over a nonlinear stretching sheet

2015

View full text Add to dashboard Cite

This article studies the Carreau viscosity model (which is a generalized Newtonian model) and then use it to obtain a formulation for the boundary layer equations of the Carreau fluid. The boundary layer flow and heat transfer to a Carreau model over a nonlinear stretching surface is discussed. The Carreau model, adequate for many non-Newtonian fluids, is used to characterize the behavior of the fluids having shear thinning properties and fluids with shear thickening properties for numerical values of the power law exponent n. The modeled boundary layer conservation equations are converted to non-linear coupled ordinary differential equations by a suitable transformation. Numerical solution of the resulting equations are obtained by using the Runge-Kutta Fehlberg method along with shooting technique. This analysis reveals many important physical aspects of flow and heat transfer. Computations are performed for different values of the stretching parameter (m), the Weissenberg number (We) and the Prandtl number (Pr). The obtained results show that for shear thinning fluid the fluid velocity is depressed by the Weissenberg number while opposite behavior for the shear thickening fluid is observed. A comparison with previously published data in limiting cases is performed and they are in excellent agreement.

show abstract

“…When using an array of closely spaced size standards (DNA ladder), the peak width does not change significantly from peak to peak, and we can use the width of a single peak (we used the first peak of the pair) instead of the average peak width. 18 For the electropherogram resulting from separation of the 100 bp ladder, the range of R bp values was 37.2-91.5 (Table 2); the average of R bp found for PeT microchips was 56 bp. Each DNA-based application has its specific requirement with respect to the separation, depending on the number and length of the DNA fragments to be resolved.…”

Section: Dna Separation Of 100 Bp Dna Ladder On Pet Microchipsmentioning

confidence: 99%

Disposable polyester–toner electrophoresis microchips for DNA analysis

Duarte

Coltro

Borba

et al. 2012

Analyst

View full text Add to dashboard Cite

Microchip electrophoresis has become a powerful tool for DNA separation, offering all of the advantages typically associated with miniaturized techniques: high speed, high resolution, ease of automation, and great versatility for both routine and research applications. Various substrate materials have been used to produce microchips for DNA separations, including conventional (glass, silicon, and quartz) and alternative (polymers) platforms. In this study, we perform DNA separation in a simple and low-cost polyester-toner (PeT)-based electrophoresis microchip. PeT devices were fabricated by a directprinting process using a 600 dpi-resolution laser printer. DNA separations were performed on PeT chip with channels filled with polymer solutions (0.5% m/v hydroxyethylcellulose or hydroxypropylcellulose) at electric fields ranging from 100 to 300 V cm À1 . Separation of DNA fragments between 100 and 1000 bp, with good correlation of the size of DNA fragments and mobility, was achieved in this system. Although the mobility increased with increasing electric field, separations showed the same profile regardless of the electric field. The system provided good separation efficiency (215 000 plates per m for the 500 bp fragment) and the separation was completed in 4 min for 1000 bp fragment ladder. The cost of a given chip is approximately $0.15 and it takes less than 10 minutes to prepare a single device.

show abstract

Principles of DNA separation with capillary electrophoresis

Cited by 195 publications

References 90 publications

Theory of DNA electrophoresis (∼ 1999 –2002 ½)

Theory of DNA electrophoresis (∼ 1999 –2002 ½)

Boundary layer flow and heat transfer to Carreau fluid over a nonlinear stretching sheet

Disposable polyester–toner electrophoresis microchips for DNA analysis

Contact Info

Product

Resources

About