Principles of Canine and Feline Blood Collection, Processing, and Storage

Abrams‐Ogg, Anthony; Blois, Shauna L.

doi:10.1002/9781119500537.ch98

Cited by 4 publications

(3 citation statements)

References 80 publications

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“…15,22 In our study, following standard veterinary recommendations for 1 year of storage, while also trying to demonstrate what practitioners are able to do when storing plasma in their own practice, storage temperature was maintained between −18° C and −25°C. 5,23 Decreasing the storage temperature of feline plasma units could improve the conservation of coagulation factors, as previously observed in human plasma storage, and thus improve the therapeutic effect. 20 However, further studies are needed to confirm this hypothesis.…”

Section: Discussionmentioning

confidence: 81%

Stability of coagulation factors in feline fresh frozen plasma intended for transfusion after 1 year of storage

Blasi-Brugué

Ferreira

Sánchez

et al. 2022

Journal of Feline Medicine and Surgery

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Objectives The most common use of plasma transfusion is for haemostatic purposes, but coagulation factor activities in stored feline plasma are unknown. The concentration and stability of coagulation factors I (fibrinogen), II, V, VII, VIII, IX, X, XI and XII in feline fresh frozen plasma (fFFP) stored for 1 year were studied. Methods Fifty-five units of fFFP were produced from 55 fresh whole-blood donations obtained from indoor healthy blood donor cats. Twenty-one units were stored for <2 weeks (T0) and 34 were stored for 1 year (T1). After the completion of storage, specific coagulation factor activities for factors II, V, VII, VIII, IX, X, XI and XII were tested using modified one-stage activated partial thromboplastin or prothrombin time assays. Fibrinogen was determined using the Clauss method. Results Significantly decreased activities were observed for factors II (T0: 101.94% ± 19.06%; T1: 73.23% ± 39.06% [ P = 0.001]), VII (T0: 102.78% ± 24.69%; T1: 60.08% ± 38.17% [ P <0.001]), VIII (T0: 77.52% ± 30.39%; T1: 50.32% ± 23.8% [ P = 0.001]), XI (T0: 88.76% ± 22.73%; T1: 66.28% ± 22.2% [ P = 0.001]) and XII (T0: 89.50% ± 21.85%; T1: 55.46% ± 23.18% [ P <0.001]) when comparing units at time 0 and after 1 year of storage. No significant difference was observed for factors IX (T0: 84.86% ± 29.35%; T1: 71.37% ± 22.23% [ P = 0.064]) and X (T0: 96.24% ± 25.1%; T1: 83.91% ± 49.54% [ P = 0.236]). Unexpectedly, a significant increase was observed for factor V (T0: 71.94% ± 24.14%; T1: 97.89% ± 62.33%; P = 0.046). Fibrinogen was 2.76 ± 1.09 g/l at T1. Factors VIII, XII and VII had the lowest mean activities after 1 year. Conclusions and relevance Although a decrease in most coagulation factors activities was noted with storage, 1-year-old fFFP was haemostatically active in vitro. The most suitable factors for quality control assessment of fFFP are factors VII and VIII. Approximately 13–20 ml/kg of fFFP is required to administer a minimum of 10 IU/kg coagulation factor activity.

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Section: Discussionmentioning

confidence: 81%

Stability of coagulation factors in feline fresh frozen plasma intended for transfusion after 1 year of storage

Blasi-Brugué

Ferreira

Sánchez

et al. 2022

Journal of Feline Medicine and Surgery

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“…The present study uses a CPDA-1 to blood ratio of 1 : 7 in green iguanas. A 1 : 7 ratio of CPDA-1 to blood was used in a successful homologous blood transfusion in box turtles (Mehalick et al 2007) and has been reported by other authors (Lichtenberger 2004;Abrams-Ogg and Schneider 2010). The evaluations of different anticoagulants (including CPDA-1) for short-term as well as long-term reptile blood storage were performed in alligators and sea turtles (Emerson et al 2014;Phillips et al 2017).…”

Section: Discussionmentioning

confidence: 94%

Influence of blood storage on the haematological values in captive green iguanas (Iguana iguana)

Koleckarova¹,

Knotková²,

Knotek³

2022

Vet. Med. - Czech

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The aim of this study was to evaluate the influence of long-term blood storage in citrate-phosphate-dextrose-adenine (CPDA-1) on selected haematological values in the green iguana. Seven adult captive-bred green iguanas (Iguana iguana) were enrolled in this study. Samples of CPDA-1-blood mixture (ratio 1 : 7) were kept at 4 °C for 28 days. Haematological examinations were performed immediately after the blood was collected (D0) and repeated at one-week intervals (D7, D14, D21 and D28). The total erythrocyte and leukocyte counts at D21 and D28 differed significantly from the values at D0 (P < 0.05). Significant differences (P < 0.05) in the haemoglobin concentrations (in comparison to D0) were detected at D14, D21 and D28. Starting at D14, marked erythrocyte degradation was present in the majority of the samples and various degrees of leukocyte degradation were present in all the samples. The mean haemolytic scores at D0, D7, D14, D21 and D28 were 0.14 ± 0.38, 0.71 ± 0.95, 1.43 ± 0.98, 2.14 ± 1.35 and 2.43 ± 1.62, respectively. The results indicate that it is possible to keep the whole blood of green iguanas in CPDA-1 at 4 °C for seven days, as statistically significant changes in the haematological values begin to appear on the 14<sup>th</sup> day of blood storage.

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“…The estimated feline blood volume ranges from 37 to 67 mL/kg [83,84]. In the present study, 1.9 mL of venous blood was collected for each experiment.…”

Section: Haematocrit and Haemoglobinmentioning

confidence: 99%

The Effect of a Subsequent Dose of Dexmedetomidine or Other Sedatives following an Initial Dose of Dexmedetomidine on Electrolytes, Acid–Base Balance, Creatinine, Glucose, and Cardiac Troponin I in Cats: Part II

Margeti,

Kazakos,

Galatos

et al. 2024

Veterinary Sciences

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The administered dose of dexmedetomidine may occasionally fail to produce the anticipated sedative effects. Therefore, a subsequent dose or administration of another sedative may enhance sedation; however, patient safety may be affected. The safety of seven different drugs administered at the following time point after an insufficient dose of dexmedetomidine was evaluated in a crossover, blind, experimental study that included six healthy adult cats. All cats received an initial dose of dexmedetomidine and a subsequent dose of either dexmedetomidine (Group DD), NS 0.9% (DC), tramadol (DT), butorphanol (DBT), buprenorphine (DBP), ketamine (DK), or midazolam (DM). Animal safety was assessed using repeated blood gas analysis and measurement of electrolytes, glucose, cardiac troponin I, and creatinine to evaluate cardiac, respiratory, and renal function. The median values of creatinine, cardiac troponin I, pH, partial pressure of carbon dioxide, potassium, and sodium did not change significantly throughout the study. Heart rate was significantly decreased in all groups after administration of the drug combinations, except for in the DK group. Respiratory rate decreased significantly after administration of the initial dose of dexmedetomidine and in the DBP and DM groups. The partial pressure of oxygen, although normal, decreased significantly after the administration of dexmedetomidine, whereas the median concentration of glucose increased significantly following the administration of dexmedetomidine. The results of our study suggest that the drug combinations used did not alter the blood parameters above normal limits, while cardiac and renal function were not compromised. Therefore, a safe level of sedation was achieved. However, the administration of dexmedetomidine reduced the partial pressure of oxygen; thus, oxygen supplementation during sedation may be advantageous. Additionally, the increase in glucose concentration indicates that dexmedetomidine should not be used in cats with hyperglycaemia, whereas the decrease in haematocrit suggests that dexmedetomidine is not recommended in anaemic cats.

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Principles of Canine and Feline Blood Collection, Processing, and Storage

Cited by 4 publications

References 80 publications

Stability of coagulation factors in feline fresh frozen plasma intended for transfusion after 1 year of storage

Stability of coagulation factors in feline fresh frozen plasma intended for transfusion after 1 year of storage

Influence of blood storage on the haematological values in captive green iguanas (Iguana iguana)

The Effect of a Subsequent Dose of Dexmedetomidine or Other Sedatives following an Initial Dose of Dexmedetomidine on Electrolytes, Acid–Base Balance, Creatinine, Glucose, and Cardiac Troponin I in Cats: Part II

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