Principles and Prospects of New Serological Techniques Including Immunosorbent Immunofluorescence, Immunoaffinity Isolation and Immunosorbent Enrichment for Sensitive Detection of Phytopathogenic Bacteria

Vuurde, J W van; Ruissen, M.A.; Vruggink, H.

doi:10.1007/978-94-009-3555-6_180

Cited by 8 publications

(7 citation statements)

References 9 publications

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“…Recently, selective isolation methods utilizing specific antibodies have been developed to assist in the culture of specific bacteria on non-selective or semi-selective media (Stead eral., 1987;van Vuurde, Ruissen and Vruggink, 1987). The approach involves the selective isolation and concentration of bacteria by immunoaffinity chromatography .…”

Section: Selective Mediamentioning

confidence: 99%

“…Another approach is to develop and use group-specific assays to identify unknown pathogens, e.g. for ISEM or immunosorbent immunofluorescence (van Vuurde, Ruissen and Vruggink, 1987) one could use a mixture of group-specific antisera to trap the unknown pathogen. After visualization and determination of the group affiliation based on other properties of the pathogen, one can then systematically decorate the same specimen with more specific antibodies.…”

Section: Future Developmentsmentioning

confidence: 99%

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New Approaches to the Detection of Microbial Plant Pathogens

Chu

Waterhouse

Martín

et al. 1989

Biotechnology and Genetic Engineering Reviews

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Section: Selective Mediamentioning

confidence: 99%

Section: Future Developmentsmentioning

confidence: 99%

New Approaches to the Detection of Microbial Plant Pathogens

Chu

Waterhouse

Martín

et al. 1989

Biotechnology and Genetic Engineering Reviews

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“…Currently, crop diseases are diagnosed by visual scouting of symptoms. Generally, disease confirmation is performed using serological and molecular methods such as: Enzyme-Linked ImmuneSorbent Assay (ELISA), western blots, immuno-strip assays, dot-blot and specific electron microscopy (Van Vuurde et al, 1987). ELISA techniques through both poly-and monoclonal antibodies are useful to identification of many bacteria.…”

Section: Detection Methods Of Plant Diseasesmentioning

confidence: 99%

Proposal of a Genome Editing System for Genetic Resistance to Tomato Spotted Wilt Virus

Martinelli¹,

Grillone²,

Sgroi³

2014

American Journal of Applied Sciences

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Viruses provoke considerable economical losses in agriculture. New molecular approaches to develop genetic resistance based on translational genomics and precision genetic modifications are highly expected. The type II Clustered, Regularly Interspaced Palindromic Repeats (CRISPR) system including Cas9 nuclease represent a promising and very powerful tool to specifically modulate the expression and activity of genes involved in biotic stress responses. In this study, we describe an approach to develop a platform system based on CRISPR system for genome editing technology in tomato. Tomato is an excellent plant for this approach considering the high-quality genome sequence, the rapid life cycle, the highly efficient in vitro plant culture and transformation protocols, Genome editing can be used to allow resistance to Tomato Spotted Wilt Virus (TSWV) infections by the successful obtainment of two specific objectives: (1) development of a Genome Editing (GE) system using CRISPR-Cas9 system in tomato (Objective 1) and (2) test the system in inducing genetic resistance to TSWV infections. First, it will be necessary to model the molecular dynamics of key host and pathogen proteins predicting how targeted mutations affect these interactions. Then these host players will be targeted by CRISPR-Cas9 technology. The obtained plants can be evaluated for their phenotypic resistance and deeply analyzed using "omic" platforms to gain insight into gene regulatory networks of plant resistance. Outcomes of the proposed project will be essentially three: (1) Identify host target proteins interacting with pathogenic proteins and model their dynamic interactions; (2) develop a platform technology usable to obtain resistant tomatoes to TSWV inducing targeted genetic modifications in the genome; (3) facilitate the adaption of this platform to the improvement of important traits in other specialty crops.

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“…5 mm) commercial affinity beads (Biorad, Pharmacia) or large (1-3 mm) polystyrene adsorbing beads, from which immunotrapped bacteria can be desorbed and plated. Concentration of the immunoselected bacteria present in the column eluent can be obtained by centrifugation followed by plating of the dissolved pellet, or by filtration through a sterile 0.45 pm millipore filter subsequently placed on the surface of a suitable agar medium to allow colony formation Van Vuurde et al, 1987).…”

Section: Solid Phases and Procedures For Immunoisolationmentioning

confidence: 99%

“…Selective desorption procedures of (blood) cells after immunobinding are described by Pharmacia (1980). The procedure for immunoisolation of phytopathogenic bacteria is partly described and discussed elswhere (Van Vuurde & Van Henten, 1983;Van Vuurde et al, 1987).…”

Section: Solid Phases and Procedures For Immunoisolationmentioning

confidence: 99%

New approach in detecting phytopathogenic bacteria by combined immunoisolation and immunoidentification assays¹

Vuurde¹

1987

EPPO Bulletin

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Antibodies coated onto a suitable solid phase, e.g. polystyrene beads, rods for inoculating agar plates, or petri dishes, enable selective trapping of homologous bacteria and of immunologically related bacteria by immunoaffinity. After washing away unbound organisms, the bound organisms are desorbed and plated on a suitable medium. Selective immunoisolation has been demonstrated for various phytopathogenic bacteria including Corynebacterium michiganense ssp. michiganense, Erwinia chrysanthemi, E. carotovora ssp. atroseptica, Xanthomonas campestris pv. begoniae and Pseudomonas syringae pv. phaseolicola. Three serological methods are described for additional rapid immunoidentification of colonies directly on agar plates and for screening for cross-reacting microorganisms: (a) direct immunodiffusion on dilution plates, (b) agar mixed-antibody assay, (c) fluorescent antibody colony staining. Schemes are presented for increasing the reliability and sensitivity of sample screening for quality indexing of plant material, and for efficient screening and isolation of possible cross-reacting microorganisms to enable production of more specific antisera.

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Principles and Prospects of New Serological Techniques Including Immunosorbent Immunofluorescence, Immunoaffinity Isolation and Immunosorbent Enrichment for Sensitive Detection of Phytopathogenic Bacteria

Cited by 8 publications

References 9 publications

New Approaches to the Detection of Microbial Plant Pathogens

New Approaches to the Detection of Microbial Plant Pathogens

Proposal of a Genome Editing System for Genetic Resistance to Tomato Spotted Wilt Virus

New approach in detecting phytopathogenic bacteria by combined immunoisolation and immunoidentification assays¹

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Principles and Prospects of New Serological Techniques Including Immunosorbent Immunofluorescence, Immunoaffinity Isolation and Immunosorbent Enrichment for Sensitive Detection of Phytopathogenic Bacteria

Cited by 8 publications

References 9 publications

New Approaches to the Detection of Microbial Plant Pathogens

New Approaches to the Detection of Microbial Plant Pathogens

Proposal of a Genome Editing System for Genetic Resistance to Tomato Spotted Wilt Virus

New approach in detecting phytopathogenic bacteria by combined immunoisolation and immunoidentification assays1

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New approach in detecting phytopathogenic bacteria by combined immunoisolation and immunoidentification assays¹