1991
DOI: 10.1016/0163-7258(91)90022-e
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Principles and practice of DNA filter elution

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Cited by 148 publications
(97 citation statements)
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“…ONOO Ϫ can also cause OHdG lesions through the formation of ⅐OH-like intermediates (Coddington et al, 1998). OHdG-DNA and ssDNA lesions in injured MNs could be related, because OH-DNA adducts can lead to abasic sites that are converted to DNA single-strand breaks (Kohn, 1991). The formation of ssDNA in preapoptotic MNs is consistent with a p53-dependent, Fas-regulated cell death mechanism.…”
Section: Onoosupporting
confidence: 49%
“…ONOO Ϫ can also cause OHdG lesions through the formation of ⅐OH-like intermediates (Coddington et al, 1998). OHdG-DNA and ssDNA lesions in injured MNs could be related, because OH-DNA adducts can lead to abasic sites that are converted to DNA single-strand breaks (Kohn, 1991). The formation of ssDNA in preapoptotic MNs is consistent with a p53-dependent, Fas-regulated cell death mechanism.…”
Section: Onoosupporting
confidence: 49%
“…Analysis of drug-induced DNA strand breaks was performed using the filter alkaline elution technique (Kohn, 1991) as already described (Montaudon et al, 1997). The cells were labelled with [methyl-3 H]thymidine (0.1 mCi ml -1 medium, Amersham Pharmacia Biotech), then treated with different drug concentrations for 2 h at 37˚C.…”
Section: Evaluation Of Dna Damage In C6 Cellsmentioning
confidence: 99%
“…micronuclei, mutations, structural chromosomal aberrations) that result from DNA damage, have been used to identify substances with genotoxic activity. This includes the most frequently used methods either the detection of DNA repair synthesis (unscheduled DNA synthesis or UDS) in individual cells, or the detection of DNA single strand breaks and alkaline labile sites in pooled cell populations using the alkaline elution assay (Kohn, 1991). A more practical and useful approach for assessing DNA damage is the single cell gel electrophoresis (SCGE) or the Comet assay (Singh et al, 1988).…”
Section: Introductionmentioning
confidence: 99%