Principles and Challenges Involved in the Enzymatic Hydrolysis of Cellulosic Materials at High Total Solids

Fockink, Douglas H.; Urio, Mateus Barbian; Chiarello, Luana Marcele; Sánchez, Jorge H.; Ramos, Luiz Pereira

doi:10.1007/978-3-319-30205-8_7

Cited by 12 publications

(7 citation statements)

References 114 publications

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“…Indeed, the wet waste pulp of red beet is a complex matrix and consists mainly of the plant cell wall polysaccharides (pectin, cellulose, hemicellulose), lignin, other small organic molecules (such as carbohydrates, betalains, and polyphenols), and inorganic ions (such as Ca 2+ , K + , and Na + ). Enzymatic pretreatment of agrifood waste with appropriate hydrolyzing enzymes is an already established approach . For instance, Papaioannou and Karabelas studied lycopene recovery from tomato peel under mild conditions assisted by enzymatic pretreatment and nonionic surfactants, thereby allowing disruption of the cell wall structure for enhanced recovery of compounds from plant cell walls.…”

Section: Introductionmentioning

confidence: 99%

“…Enzymatic pretreatment of agrifood waste with appropriate hydrolyzing enzymes is an already established approach. 27 For instance, Papaioannou and Karabelas 28 studied lycopene recovery from tomato peel under mild conditions assisted by enzymatic pretreatment and nonionic surfactants, thereby allowing disruption of the cell wall structure for enhanced recovery of compounds from plant cell walls.…”

Section: ■ Introductionmentioning

confidence: 99%

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Application of an Ultrasound-Assisted Extraction Method to Recover Betalains and Polyphenols from Red Beetroot Waste

Fernando

Wood

Papaioannou

et al. 2021

ACS Sustainable Chem. Eng.

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Section: Introductionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

Application of an Ultrasound-Assisted Extraction Method to Recover Betalains and Polyphenols from Red Beetroot Waste

Fernando

Wood

Papaioannou

et al. 2021

ACS Sustainable Chem. Eng.

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“…The cellulolytic system of Trichoderma reesei is well established and exploited at commercial level in textile and biofuel industries. However, T. reesei produces low amount of extracellular β-glucosidase renders partial hydrolysis of cellulosic material [7,8]. Therefore commercial enzymes are supplemented with β-glucosidase from Aspergillus niger [9].…”

Section: Introductionmentioning

confidence: 99%

Hydrolytic Potential of Cellulases from Pénicillium funiculosum and Trichoderma reesei against PhysicoChemically Different Feedstocks

Aa¹

2017

AIBM

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Competitive alternatives for cellulase producing organism for the development of efficient enzyme mixture should be explored to reduce the cost of commercial cellulases in the enzymatic hydrolysis of lignocellulosic biomass. Cellulolytic enzyme mixtures were produced using Penicillium funiculosum and Trichoderma reesei on ammonia treated wheat straw. Cellulolytic assay using crude extract obtained from P. funiculosum showed 36%, 181% and 370% higher FPase, CMCase and β-glucosidase specific activity compared to T. reesei. We compared enzyme mixtures of these two organisms against differently treated wheat straw, ammonia treated rice straw and bagasse. Culture supernatants obtained from both fungi showed equal hydrolytic performance among ammonia treated feedstock. However, P. funiculosum enzyme has released nearly 8-10% high sugar from rice straw and bagasse than T. reesei enzymes. Pretreatment catalysts like nitric, ammonia, caustic showed around 10% less hydrolysis using T. reesei enzyme extract, than P. funiculosum derived enzyme extracts. Here, we found that T. reesei with lower β-glucosidase causes decreased glucan and xylan hydrolyzing capacity than enzyme extract of P. funiculosum. However, this lower glucan yield is compensated for high cellobiose yield using T. reesei derived enzymes. Caustic pretreatment showed higher digestibility irrespective of enzyme source. Finally supplementation of β-glucosidase showed improvement in high glucose release and ultimately hydrolytic performance by more than 20%.

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“…One approach to improve the economic feasibility of the process is to increase the amount of biomass in the enzymatic hydrolysis reaction, which is referred to as "high-solids" enzymatic hydrolysis [12][13][14]. A process can be considered "high-solids" when the content of insoluble solids is such that no free water is present in the slurry at the onset of the hydrolysis reaction, which is generally observed at solids loadings higher than 15% (w/w) dry matter for most pretreated materials [15][16][17]. The use of high-solids content benefits the economics of lignocellulosic biomass conversion to fuels and chemicals by decreasing both capital and operational costs, as the increase in the final product concentration reduces equipment volumes alongside the costs for the separation steps, the water consumption, the wastewater generation, and the subsequent cost for disposal [18,19].…”

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confidence: 99%

Constraints and advances in high-solids enzymatic hydrolysis of lignocellulosic biomass: a critical review

Silva

Espinheira

Teixeira

et al. 2020

Biotechnol Biofuels

147

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The industrial production of sugar syrups from lignocellulosic materials requires the conduction of the enzymatic hydrolysis step at high-solids loadings (i.e., with over 15% solids [w/w] in the reaction mixture). Such conditions result in sugar syrups with increased concentrations and in improvements in both capital and operational costs, making the process more economically feasible. However, this approach still poses several technical hindrances that impact the process efficiency, known as the “high-solids effect” (i.e., the decrease in glucan conversion yields as solids load increases). The purpose of this review was to present the findings on the main limitations and advances in high-solids enzymatic hydrolysis in an updated and comprehensive manner. The causes for the rheological limitations at the onset of the high-solids operation as well as those influencing the “high-solids effect” will be discussed. The subject of water constraint, which results in a highly viscous system and impairs mixing, and by extension, mass and heat transfer, will be analyzed under the perspective of the limitations imposed to the action of the cellulolytic enzymes. The “high-solids effect” will be further discussed vis-à-vis enzymes end-product inhibition and the inhibitory effect of compounds formed during the biomass pretreatment as well as the enzymes’ unproductive adsorption to lignin. This review also presents the scientific and technological advances being introduced to lessen high-solids hydrolysis hindrances, such as the development of more efficient enzyme formulations, biomass and enzyme feeding strategies, reactor and impeller designs as well as process strategies to alleviate the end-product inhibition. We surveyed the academic literature in the form of scientific papers as well as patents to showcase the efforts on technological development and industrial implementation of the use of lignocellulosic materials as renewable feedstocks. Using a critical approach, we expect that this review will aid in the identification of areas with higher demand for scientific and technological efforts.

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Principles and Challenges Involved in the Enzymatic Hydrolysis of Cellulosic Materials at High Total Solids

Cited by 12 publications

References 114 publications

Application of an Ultrasound-Assisted Extraction Method to Recover Betalains and Polyphenols from Red Beetroot Waste

Application of an Ultrasound-Assisted Extraction Method to Recover Betalains and Polyphenols from Red Beetroot Waste

Hydrolytic Potential of Cellulases from Pénicillium funiculosum and Trichoderma reesei against PhysicoChemically Different Feedstocks

Constraints and advances in high-solids enzymatic hydrolysis of lignocellulosic biomass: a critical review

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