Primordial germ cell migration

Molyneaux, Kathleen; Wylie, Christopher

doi:10.1387/ijdb.041833km

Cited by 220 publications

(164 citation statements)

References 43 publications

(70 reference statements)

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“…As PGCs in different organisms appear to utilize similar molecular pathways for their migration (Molyneaux and Wylie, 2004;Raz, 2004;Santos and Lehmann, 2004), the results described here may be relevant for mouse and Drosophila PGCs, as well. The finding that early zygotically transcribed genes and those whose function is regulated by the Dead end protein are essential for PGC transition from non-motile to directionally migrating cells should allow focusing the search for the specific molecules involved in this process.…”

Section: Discussionmentioning

confidence: 77%

“…In many organisms, primordial germ cells (PGCs) are specified early in development and subsequently migrate towards the region of the gonad where they give rise to gametes (Molyneaux and Wylie, 2004;Raz, 2004;Santos and Lehmann, 2004). We study PGC development in zebrafish, a vertebrate model organism whose germline is specified through the inheritance of asymmetrically localized maternally provided determinants, collectively termed germ plasm (Knaut et al, 2000;Yoon et al, 1997).…”

Section: Introductionmentioning

confidence: 99%

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Transition from non-motile behaviour to directed migration during early PGC development in zebrafish

Blaser

Eisenbeiß

Neumann

et al. 2005

Journal of Cell Science

165

189

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The migration of zebrafish primordial germ cells (PGCs) is directed by SDF-1a and serves as a model for long-range chemokine-guided cell migration. Whereas the development and migration of zebrafish PGCs have been studied in great detail starting at mid-gastrulation stages when the cells exhibit guided active migration [7-8 hours post fertilization (hpf)], earlier stages have not yet been examined. Here we show that the PGCs acquire competence to respond to the chemokine following discrete maturation steps. Using the promoter of the novel gene askopos and RNA elements of nanos1 to drive GFP expression in PGCs, we found that immediately after their specification (about 3 hpf) PGCs exhibit simple cell shape. This stage is followed by a phase at which the cells assume complex morphology yet they neither change their position nor do they respond to SDF-1a. During the third phase, a transition into a `migratory stage' occurs as PGCs become responsive to directional cues provided by somatic cells secreting the chemokine SDF-1a. This transition depends on zygotic transcription and on the function of the RNA-binding protein Dead end and is correlated with down regulation of the cell adhesion molecule E-cadherin. These distinctive morphological and molecular alterations could represent a general occurrence in similar processes critical for development and disease.

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Section: Discussionmentioning

confidence: 77%

Section: Introductionmentioning

confidence: 99%

Transition from non-motile behaviour to directed migration during early PGC development in zebrafish

Blaser

Eisenbeiß

Neumann

et al. 2005

Journal of Cell Science

165

189

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show abstract

“…1 and Fig. 2), and it is believed that different embryonic mesenchymal cells during migration generally follow the mechanisms described in the first section of the review (Gerthoffer, 2007;Molyneaux and Wylie, 2004;Pak et al, 2008) (Fig. 2).…”

Section: Cell Migration In the Developing Embryo Major Migratory Cellmentioning

confidence: 94%

“…This migration takes place over a considerable period of time (5 embryonic days for a mouse embryo, which constitutes approximately ¼ of the entire development), and is believed to occur in stages of rapid migration followed by relatively stationary periods (Molyneaux and Wylie, 2004;Molyneaux et al, 2001). The actual speed of this migration is difficult to estimate, because as the cells migrate, the embryo itself continues to grow and increases several times in size during the migration process (Fig.…”

Section: Embryonic Migratory Eventsmentioning

confidence: 99%

Cell biology of embryonic migration

Kurosaka¹,

Kashina²

2008

Birth Defects Research Pt C

113

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Cell migration is an evolutionarily conserved mechanism that underlies the development and functioning of uni-and multicellular organisms and takes place in normal and pathogenic processes, including various events of embryogenesis, wound healing, immune response, cancer metastases, and angiogenesis. Despite the differences in the cell types that take part in different migratory events, it is believed that all of these migrations occur by similar molecular mechanisms, whose major components have been functionally conserved in evolution and whose perturbation leads to severe developmental defects. These mechanisms involve intricate cytoskeleton-based molecular machines that can sense the environment, respond to signals, and modulate the entire cell behavior. A big question that has concerned the researchers for decades relates to the coordination of cell migration in situ and its relation to the intracellular aspects of the cell migratory mechanisms. Traditionally, this question has been addressed by researchers that considered the intra-and extracellular mechanisms driving migration in separate sets of studies. As more data accumulate researchers are now able to integrate all of the available information and consider the intracellular mechanisms of cell migration in the context of the developing organisms that contain additional levels of complexity provided by extracellular regulation. This review provides a broad summary of the existing and emerging data in the cell and developmental biology fields regarding cell migration during development.

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“…Thus, precursors of PGC are the first population of stem cells that is specified in the embryo at the beginning of gastrulation. PGC in mice subsequently move for a short period of time first to the basis of alantois, which is located in the extraembryonic mesoderm and then migrate into the embryo proper toward the genital ridges-where they will undergo developmental differentiation to oocytes or sperm respectively (Molyneaux and Wylie, 2004; Fig. 1).…”

Section: Germ Lineage a Mother Lineage Of All Cell Lineages In The Bodymentioning

confidence: 99%

Bone marrow‐derived very small embryonic‐like stem cells: Their developmental origin and biological significance

Kucia

Wan

Ratajczak

2007

Developmental Dynamics

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Data from our and other laboratories provide evidence that bone marrow (BM) contains a population of stem cells that expresses early developmental markers such as (1) stage-specific embryonic antigen (SSEA) and (2) transcription factors Oct-4 and Nanog. These are the markers characteristic for embryonic stem cells, epiblast stem cells, and primordial germ cells (PGC). The presence of these stem cells in adult BM supports the concept that this organ contains some population of pluripotent stem cells that is deposited in embryogenesis during early gastrulation. We hypothesize that these cells could be direct descendants of the germ lineage that, to pass genes on to the next generations, has to create soma and, thus, becomes a "mother lineage" for all somatic cell lineages present in the adult body. Germ potential is established after conception in totipotent zygotes and retained in blastomeres of morula, cells from the inner cell mass of blastocyst, epiblast, and population of PGC. We will present a concept that SSEA ؉ Oct-4 ؉ Nanog ؉ cells identified in BM could be descendants of epiblast cells as well as some rare migrating astray PGC. Developmental Dynamics 236:3309 -3320, 2007.

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Primordial germ cell migration

Cited by 220 publications

References 43 publications

Transition from non-motile behaviour to directed migration during early PGC development in zebrafish

Transition from non-motile behaviour to directed migration during early PGC development in zebrafish

Cell biology of embryonic migration

Bone marrow‐derived very small embryonic‐like stem cells: Their developmental origin and biological significance

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