Primordial follicle assembly was regulated by notch signaling pathway in the mice

Chen, Chunlei; Fu, Xiafei; Wang, Linqing; Wang, Junjie; Ma, Haifeng; Cheng, Shun‐Feng; Hou, Zhu-Mei; Ma, Jin-Mei; Quan, Guobo; Shen, Wei; Li, Lan

doi:10.1007/s11033-014-3038-4

Cited by 42 publications

(22 citation statements)

References 43 publications

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“…Therefore, in the present study, we observed two critical periods during the formation and transformation of primordial follicles. Early studies found that Notch signaling mediates primordial follicle formation and transformation, particularly involving the SOHLH2, NOBOX and LHX8 transcription factors [26,27]. Early studies found that downregulation of mRNA expression of sohlh2 , nobox and lhx 8 alters primordial follicle numbers and results in a lifelong reduction in the primordial follicle pool [26].…”

Section: Discussionmentioning

confidence: 99%

“…Early studies found that Notch signaling mediates primordial follicle formation and transformation, particularly involving the SOHLH2, NOBOX and LHX8 transcription factors [26,27]. Early studies found that downregulation of mRNA expression of sohlh2 , nobox and lhx 8 alters primordial follicle numbers and results in a lifelong reduction in the primordial follicle pool [26]. Interestingly, in the present study, we did not observe a difference in the gene expression level of sohlh2 , nobox and lhx 8 in the fetal ovary.…”

Section: Discussionmentioning

confidence: 99%

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Effect of High Fat Dietary Intake during Maternal Gestation on Offspring Ovarian Health in a Pig Model

Che

Yang

et al. 2016

Nutrients

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Excessive fat intake is a global health concern as women of childbearing age increasingly ingest a high fat diet. We therefore determined the association of a maternal high fat diet in pregnancy with offspring ovarian health during the gestation and postnatal female offspring in pig a model. Thirty-two Yorkshire gilts with similar bodyweights mated at the third estrus were randomly assigned to two nutrition levels of either a control (CON, crude fat: 7.27%) or a high fat diet (HFD, crude fat: 11.78%). Ovary samples were collected during the fetal (Day 55 (g55) and Day 90 of gestation (g90)) and offspring (prepuberty Day 160 (d160) and age at puberty) period to detect ovary development, antioxidant status and apoptosis cells. Maternal HFD did not influence notch signaling gene expression, which regulates primordial follicle formation and transformation, and ovarian histological effect at g55 and g90. However, maternal HFD reduced the numbers of large follicles at d160 and small follicle numbers upon puberty compared to CON in offspring. The results also revealed that the antioxidant index of total antioxidative capability (T-AOC), cytoplasmic copper/zinc superoxide dismutase (CuZn-SOD), glutathione peroxidase (GPx) activities and mRNA expression were higher in the CON than the HFD at g90 and d160, whereas, malondialdehyde (MDA) concentration was decreased in the CON. Maternal HFD increased the inhibitor of the apoptosis-related gene of B-cell lymphoma-2 (bcl2) mRNA expression at g90 and d160, whereas, pro-apoptotic-related gene bcl-2 assaciated X protein (bax) was reduced. These data show that the maternal high fat diet does not delay fetal ovarian development, but it changes ovarian health by the induction of oxidative stress and accelerating cell apoptosis in offspring.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Effect of High Fat Dietary Intake during Maternal Gestation on Offspring Ovarian Health in a Pig Model

Che

Yang

et al. 2016

Nutrients

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“…The previous study reported rodent ovary culture has been successfully performed by other investigators to confirm roles for signaling molecules during early follicle formation [24,27,52]. We utilize an in vivo follicle formation model in this system, arguing that factors important for follicle assembly are important to ovarian development.…”

Section: Discussionmentioning

confidence: 99%

“…On the other hand, the Hippo signaling pathway, which consists of several oncogenes (MST1/LATS2/YAP1)could sense the changes of cell density in Drosophila ovaries [22]. The Hippo signaling pathway forms a cross network with a variety of factors and multiple signaling pathways such as Notch, TGFβ, cTGF, IGF, c-erbB2, c-myc, and PI3K/AKt in different levels [23,24,25,26,27]. Our earlier reports demonstrated that the physiological effect of Pten and Tsc1/mTorc1 signaling pathways, which have been proved to maintain of the quiescence of primordial follicles, included anti-oncogenes that activated in the course of tumorigenesis [28,29,30,31].…”

Section: Introductionmentioning

confidence: 99%

Hippo Signaling Pathway Reveals a Spatio-Temporal Correlation with the Size of Primordial Follicle Pool in Mice

Xiang

et al. 2015

Cell Physiol Biochem

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Background: The Hippo signaling pathway, a highly conserved cell signaling system, exists in most multicellular organisms and regulates cell proliferation, differentiation, and apoptosis. It has been reported that the members of Hippo signaling are expressed in mammalian ovaries, but the exact functions of this pathway in primordial follicle development remains unclear. Methods: To analyze the spatio-temporal correlation between the core component of Hippo pathway and the size of primordial follicle pool, Western blot, Real-time PCR and immunohistochemistry were used, and the expression and localization of MST1, LATS2 and YAP1 mRNA and protein were examined in 3 d, 1 m, 5 m, 16 m postnatal mice ovary and the culture model of mice primordial follicle in vitro. Results: Both the protein and mRNA expression of the MST1 and LATS2 were decreased significantly as mouse age increased (p < 0.05), however, the mRNA expression of them increased significantly in 16 m compared with 5 m as well as the protein expression of LATS2.The expression of YAP showed the opposite trend, and the significant protein expression of pYAP was increased before 1 m, after which no significant change was observed. Moreover, the ratio of pYAP/YAP decreased significantly. Culturing ovaries for 8 d in vitro resulted in the activation of primordial follicles in 3 d postnatal mice ovaries, and these developed into primary follicles with the expression of PCNA increasing significantly (p < 0.05). The mRNA and protein expression of MST and LATS decreased significantly (p < 0.05), and the expression of YAP increased significantly (p < 0.05, p < 0.01), whereas the ratio of pYAP/YAP decreased significantly (p < 0.05). Conclusion: The above results reveal that the expression of the core components of Hippo pathway changed during mouse follicular development, especially before and after primordial follicle activation in vitro. The primordial follicle activation may be related to the significant decrease of the ratio of pYAP1/YAP1. In conclusion, Hippo signaling pathway expressed in mice ovaries and have spatio-temporal correlation with the size of primordial follicle pool.

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“…At the end of the culturing, the ovarian tissues were collected and washed three times with PBS for the indicated analyses. Oocytes were isolated from the cultured ovarian tissues as previously described [23][24][25][26][27].…”

Section: Ovarian Culturementioning

confidence: 99%

Activin A Accelerates the Progression of Fetal Oocytes Throughout Meiosis and Early Oogenesis in the Mouse

Liang

Zhang

Wang

et al. 2015

Stem Cells and Development

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Activins can exert several roles in ovary development. However, little is known about their involvement in early mammalian oogenesis. In this study, we reported that activin receptors (including ActRIA, ActRIB, ActRIIA, and ActRIIB) are expressed throughout the development of the mouse ovaries from 12.5 days postcoitum (dpc) to 21 days postparturition (dpp). Moreover, we found that in vitro, the addition of activin A (ActA) to the culture medium of 12.5 dpc ovarian tissues accelerated the progression of oocytes throughout meiotic prophase I stages. This result was reproduced in vivo following administration of ActA to pregnant mice. The in vitro effect of ActA was associated with increased expression of premeiotic and meiotic genes (including Dazl, Spo11, Stra8, Scp3, and Rec8) in the ovarian tissues. Mechanistically, ActA-dependent SMAD3 signaling modulated the expression of members of the retinoic acid (RA) system, including the RA degradation CYP26B1 enzyme and the RA receptors. Finally, ActA promoted the survival and growth of fetal and early postnatal oocytes and primordial follicle assembly both in vitro and in vivo. In conclusion, the present study identifies new roles of ActA in early oogenesis and suggested that ActA and RA might cooperate in promoting meiosis in female germ cells.

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Primordial follicle assembly was regulated by notch signaling pathway in the mice

Cited by 42 publications

References 43 publications

Effect of High Fat Dietary Intake during Maternal Gestation on Offspring Ovarian Health in a Pig Model

Effect of High Fat Dietary Intake during Maternal Gestation on Offspring Ovarian Health in a Pig Model

Hippo Signaling Pathway Reveals a Spatio-Temporal Correlation with the Size of Primordial Follicle Pool in Mice

Activin A Accelerates the Progression of Fetal Oocytes Throughout Meiosis and Early Oogenesis in the Mouse

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