Priming the prophenoloxidase system of Artemia franciscana by heat shock proteins protects against Vibrio campbellii challenge

Baruah, Kartik; Ranjan, Jayant; Sorgeloos, Patrick; MacRae, Thomas H.; Bossier, Peter

doi:10.1016/j.fsi.2011.04.008

Cited by 50 publications

(36 citation statements)

References 51 publications

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“…3-5 were performed. In accordance with the findings of our previous studies (Baruah et al, 2011;Sung et al, 2007), Hspi treatment markedly increased Hsp70 production in the brine shrimp larvae as compared to the control (Fig. 3).…”

Section: Putative Mechanism Of Hsp70 Induction By Hspisupporting

confidence: 92%

“…We previously reported that feeding of recombinant Hsp70 elicits protective innate immunity in the brine shrimp host (Baruah et al, 2011). Because Hsp70 was strongly induced by Hspi, we next investigated the effect of Hspi on the temporal expression of the proPO and tgase immune genes in the shrimp larvae.…”

Section: Prophenoloxidase (Propo) and Transglutaminase (Tgase) Immunementioning

confidence: 99%

“…One potential new approach is offered by the discovery of molecular chaperones or heat shock proteins (Hsp), and their cytoprotective potential (Almeida et al, 2011;Baruah et al, 2011;Powers et al, 2010). Hsps, mainly the 70 kDa Hsp (Hsp70), are highly conserved proteins of which expression is either constitutive or inducible under different conditions.…”

Section: Introductionmentioning

confidence: 99%

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Reactive oxygen species generated by a heat shock protein (Hsp) inducing product contributes to Hsp70 production and Hsp70-mediated protective immunity in Artemia franciscana against pathogenic vibrios

Baruah

Norouzitallab

Linayati

et al. 2014

Developmental & Comparative Immunology

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Section: Putative Mechanism Of Hsp70 Induction By Hspisupporting

confidence: 92%

Section: Prophenoloxidase (Propo) and Transglutaminase (Tgase) Immunementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Reactive oxygen species generated by a heat shock protein (Hsp) inducing product contributes to Hsp70 production and Hsp70-mediated protective immunity in Artemia franciscana against pathogenic vibrios

Baruah

Norouzitallab

Linayati

et al. 2014

Developmental & Comparative Immunology

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“…Axenic Artemia cysts and nauplii were obtained following decapsulation and hatching as described previously (Baruah et al 2011;Haldar et al 2011). Briefly, 1 g of Artemia cysts of each H/D treatment and of the control was hydrated in 89 ml of distilled water for 1 h. Sterile nauplii were obtained via decapsulation of cysts using 3.3 ml NaOH (32 %) and 50 ml NaOCl (50 %).…”

Section: Axenic Hatching Of Artemiamentioning

confidence: 99%

Hydration/dehydration cycles imposed on Artemia cysts influence the tolerance limit of nauplii against abiotic and biotic stressors

et al. 2015

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The brine shrimp Artemia franciscana is an important live feed for fish and shellfish larviculture. Cysts of Artemia are naturally found in a wide variety of harsh environments worldwide where they are exposed to different changing environmental conditions. Artemia cysts are also exposed to different hydration/dehydration (H/D) conditions during the post-harvest processing period in the Artemia processing industry. In this study, cysts of two strains of A. franciscana originating from two different geographical locations-one a natural population from Great Salt Lake (GSL), Utah, USA, and the other cultured in the Vinh Chau (VC) salt ponds, Vietnam-where they experience different environmental factors, were exposed to successive hydration/dehydration (H/D) cycles with the aim of determining the effects of these conditions on the stress (i.e., induction of Hsp70) and functional (i.e., resistance toward abiotic and pathogenic biotic factors) responses of the emerged nauplii. Our results showed that a short period of H/D of the cysts did not appear to have a deleterious effect on the emerged nauplii of both strains, as was evidenced by the absence of significant difference in the survival of nauplii emerged from the control and treated cysts, upon challenge with a thermal stressor or with pathogenic Vibrio campbellii. A limited exposure to H/D treatment even leads to the induction of enhanced thermotolerance in GSL nauplii. In essence, these observations add some insights to our current understanding of stress responses in Artemia under the described experimental conditions. In addition, the impact of H/D cycles associated with stress response should be taken into consideration when Artemia is considered as a model organism for future research and applications

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“…Hsp70 has a role in the growth of A. franciscana (Clegg et al 2000;Willsie and Clegg 2002) and protection against stress, including heat and pathogenic bacteria (Sung et al 2008(Sung et al , 2009aBaruah et al 2011), but information on its major co-chaperone, Hsp40, is lacking as it is for most crustaceans. With this in mind, a search was initiated for J-domain proteins in A. franciscana resulting in the cloning and sequencing of a complementary DNA (cDNA), termed ArHsp40, encoding a type I Hsp40.…”

Section: Introductionmentioning

confidence: 99%

ArHsp40, a type 1 J-domain protein, is developmentally regulated and stress inducible in post-diapause Artemia franciscana

Jiang

Rowarth

Panchakshari

et al. 2016

Cell Stress and Chaperones

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Upon diapause termination and exposure to favorable environmental conditions, cysts of the crustacean Artemia franciscana reinitiate development, a process dependent on the resumption of metabolic activity and the maintenance of protein homeostasis. The objective of the work described herein was to characterize molecular chaperones during post-diapause growth of A. franciscana. An Hsp40 complementary DNA (cDNA) termed ArHsp40 was cloned and shown to encode a protein with an amino-terminal J-domain containing a conserved histidine, proline, and aspartic acid (HPD) motif. Following the J-domain was a Gly/Phe (G/F) rich domain, a zinc-binding domain which contained a modified CXXCXGXG motif, and the carboxyl-terminal substrate binding region, all characteristics of type I Hsp40. Multiple alignment and protein modeling showed that ArHsp40 is comparable to Hsp40s from other eukaryotes and likely to be functionally similar. qRT-PCR revealed that during postdiapause development, ArHsp40 messenger RNA (mRNA) varied slightly until the E2/E3 stage and decreased significantly upon hatching. The immunoprobing of Western blots demonstrated that ArHsp40 was also relatively constant until E2/ E3 and then declined dramatically. The drop in ArHsp40 when metabolism and protein synthesis were increasing was unexpected and demonstrated developmental regulation. The reduction in ArHsp40 at such an active life history stage indicates, as one possibility, that A. franciscana possesses additional Hsp40s, one or more of which replaces ArHsp40 as development progresses. Increased synthesis upon heat shock established that in addition to being developmentally regulated, ArHsp40 is stress inducible and, because it is found in mature cysts, ArHsp40 has the potential to contribute to stress tolerance during diapause.

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Priming the prophenoloxidase system of Artemia franciscana by heat shock proteins protects against Vibrio campbellii challenge

Cited by 50 publications

References 51 publications

Reactive oxygen species generated by a heat shock protein (Hsp) inducing product contributes to Hsp70 production and Hsp70-mediated protective immunity in Artemia franciscana against pathogenic vibrios

Reactive oxygen species generated by a heat shock protein (Hsp) inducing product contributes to Hsp70 production and Hsp70-mediated protective immunity in Artemia franciscana against pathogenic vibrios

Hydration/dehydration cycles imposed on Artemia cysts influence the tolerance limit of nauplii against abiotic and biotic stressors

ArHsp40, a type 1 J-domain protein, is developmentally regulated and stress inducible in post-diapause Artemia franciscana

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