Priming seeds-method for increasing the germination of soybean seeds under drought stress conditions

Abstract

Drought is one of the most important factors limiting the successful production of cultivated plants. One of the most sensitive stage to the water deficit in plants is seed germination. There are various methods of pre-sowing treatments that aim to reduce the negative impact of drought stress and improve seed germination. One of them is priming seeds. The aim of this experiment was to examine the effect of priming seeds in solutions - KNO3 (1%), ascorbic acid - AsA (100 mgl-1) and potassium chloride KCl (1%) on the reduction of the negative effect of drought stress. The effect of drought stress was simulated using different concentrations of PEG 6000 (0 (control), -0.30, -0.51, -0.80 MPa). The results of the research showed that with the increase of water deficiency, the effect of priming seeds is greater. At the water potential of the solution of -0.30 MPa, germination energy and seed germination increased on average by 6.77% and 5.08%, while at the water potential of the solution of -0.80 MPa, the increase was 19.28% and 16.75%, respectively. Also, priming seeds significantly reduced the intensity of lipid peroxidation and the content of free proline. From all the above, it can be concluded that priming of seeds is a method that can serve to improve the germination of soybean seeds in conditions of drought stress.

Potassium Nitrate and Ascorbic Acid Priming Improved Tissue Chemical Composition and Antioxidant and Antimicrobial Activities of Linseed (Linum usitatissimum L.) Sprouts

“…Interestingly, KNO 3 priming had the greatest effect on the seed germination when compared to AsA priming. This finding is consistent with other studies of soybean and wheat seeds . It was hypothesized that presoaking of seeds in KNO 3 would reactivate aquaporins and trigger the amylase activity that is essential for embryo development .…”

“…Besides, the APX activity is linked with the chloroplast AsA pool . Our study is also in line with many other studies which reported that exogenous application of AsA could improve the antioxidant enzyme activity. , Interestingly, priming with KNO 3 improved the accumulation of the carbohydrate content in linseed sprouts. Increased carbohydrate contents in seed sprouts justified their increased emergence, growth, and seedling vigor.…”

How long can primed soybean (Glycine max L.) seeds be stored in natron paper bags?

“…The ratio of seed volume to solution volume was 1:5 (v / v). After 6 h of priming (Miladinov et al, 2015;Miladinov et al, 2020), seeds were washed with distilled water and dried at a temperature of 25 °C to a humidity of 10-11%. The storage potential of dried primed seeds was determined by their storage at a temperature of 25 °C for 90 days.…”

Priming seeds of sorghum and sudangrass using water and aquatic extracts of willow and banana

“…On-farm seed priming increased the yield of maize in Zimbabwe and India to a tune of 22 per cent, sorghum in Zimbabwe and Pakistan to a tune of 31 per cent, wheat in India and Pakistan to a tune of 37 percent and upland rice in West Africa to a tune of 70 percent (Das et al, 2015). In numerous cases, the beneficial impact of priming on plant growth is more obvious under nonoptimal than under optimal conditions, leading to the global concept that a major advantage of priming lies in an increase in stress resistance (Miladinov et al, 2020a;Garcia et al, 2021). Miladinov et al (2018) pointed out that, in addition to the positive effect, immersion can also have a negative effect on seed germination, because the effect of immersion depends on many factors.…”