Priming by DNA immunization augments T-cell responses induced by modified live bovine herpesvirus vaccine

Loehr, B. I.; Pontarollo, Reno; Rankin, Robert; Latimer, L.; Willson, Philip; Babiuk, Lorne A.; Hurk, Sylvia van Drunen Littel‐van den

doi:10.1099/0022-1317-82-12-3035

Cited by 22 publications

(13 citation statements)

References 30 publications

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“…Despite their high BoHV-1 specific IFN-γ production and lymphocyte proliferation, the calves vaccinated three times with the pCDNA3.1VP8 showed no reduction of virus excretion as compared to the controls. This discrepancy between the cellular immune response and the virological protection after challenge contrasts with the results of studies performed with conventional vaccines [20,57] but is in agreement with observations of BoHV-1 DNA immunisation studies using other viral antigens [33,34,43]. The viral target antigens of DNA vaccines are very limited as compared to those encountered in conventional vaccines and the protective cellular immunity may be restricted to only a few proteins of BoHV-1.…”

Section: Discussionsupporting

confidence: 81%

“…Further DNA vaccination experiments performed in mice and cattle have shown that a construct encoding a secreted form of BoHV-1 gD induces a higher immune response than membrane-associated full-length gD [4,54]. Different routes of DNA administration to cattle have been tested such as intradermic or intramuscular injection [10,17,54] and biolistic delivery (gene-gun) of gold particles coated with DNA vaccines [6,33,34]. Mucosal administration of plasmid DNA via a gene-gun [34] or into suppositories [35] has also been shown to induce systemic as well as mucosal immunity.…”

Section: Introductionmentioning

confidence: 99%

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Genetic immunisation of cattle against Bovine herpesvirus 1: glycoprotein gD confers higher protection than glycoprotein gC or tegument protein VP8

et al. 2005

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-Bovine herpesvirus 1 (BoHV-1) has frequently been used as a model for testing parameters affecting DNA immunisation in large animals like cattle. However, the selection of target antigens has been poorly studied, and most of the experiments have been conducted in mice. In the present study, we demonstrated in cattle that a DNA vaccine encoding BoHV-1 glycoprotein gD induces higher neutralising antibody titres than vaccines encoding BoHV-1 gC. Additionally, we show that a DNA vaccine encoding a secreted form of gD induces a higher immune response than a vaccine encoding full-length gD. However, the enhanced immunogenicity associated with the secretion of gD could not be extended to the glycoprotein gC. The current study also describes for the first time the development and the evaluation of a DNA vaccine encoding the major tegument protein VP8. This construct, which is the first BoHV-1 plasmid vaccine candidate that is not directed against a surface glycoprotein, induced a high BoHV-1 specific cellular immunity but no humoral immune response. The calves vaccinated with the constructs encoding full-length and truncated gD showed a non-significant tenfold reduction of virus excretion after challenge. Those calves also excreted virus for significantly (p < 0.05) shorter periods (1.5 days) than the nonvaccinated controls. The other constructs encoding gC and VP8 antigens induced no virological protection as compared to controls. Altogether the DNA vaccines induced weaker immunity and protection than conventional marker vaccines tested previously, confirming the difficulty to develop efficient DNA vaccines in large species.BoHV-1 / DNA immunisation / gC / gD / VP8

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Section: Discussionsupporting

confidence: 81%

Section: Introductionmentioning

confidence: 99%

Genetic immunisation of cattle against Bovine herpesvirus 1: glycoprotein gD confers higher protection than glycoprotein gC or tegument protein VP8

et al. 2005

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“…Indeed, strong cellular responses in the absence of high antibody titers do not fully protect against infection (25,26). In contrast, calves fed freeze-thawed colostrum containing high levels of neutralizing antibodies to BHV-1 but no viable immune cells were fully protected against fatal multisystemic infectious rhinotracheitis (29).…”

Section: Discussionmentioning

confidence: 99%

The Immunogenicity and Protective Efficacy of Bovine Herpesvirus 1 Glycoprotein D plus Emulsigen Are Increased by Formulation with CpG Oligodeoxynucleotides

Ioannou¹,

Griebel²,

Hecker

et al. 2002

J Virol

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118

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The immunogenicity and protective efficacy of a bovine herpesvirus 1 (BHV-1) subunit vaccine formulated with Emulsigen (Em) and a synthetic oligodeoxynucleotide containing unmethylated CpG dinucleotides (CpG ODN) was determined in cattle. A truncated, secreted version of BHV-1 glycoprotein D (tgD) formulated with Em and CpG ODN at concentrations of 25, 2.5, or 0.25 mg/dose produced a more balanced immune response, higher levels of virus neutralizing antibodies, and greater protection after BHV-1 challenge compared to tgD adjuvanted with either Em or CpG ODN alone. In contrast, tgD formulated with Em and either 25 mg of a non-CpG ODN or another immunostimulatory compound, dimethyl dioctadecyl ammonium bromide, induced similar immunity and protection compared to tgD formulated with Em alone, a finding which confirms the immunostimulatory effect of ODN to be CpG motif mediated. Our results demonstrate the ability of CpG ODN to induce a strong and balanced immune response in a target species.

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“…To further analyze the immune responses induced by the two different constructs, we determined the lymphocyte proliferative responses (19). After primary as well as secondary immunization, the tgD-specific proliferative responses of both plasmid-vaccinated groups were superior to those of the control group (P Ͻ 0.001).…”

Section: Figmentioning

confidence: 99%

“…1. (a) Mean tgD-specific IgG titers Ϯ SEM, determined by ELISA as previously described (19). (b) Mean tgD-specific IgA titers Ϯ SEM, determined by ELISA (19).…”

Section: Figmentioning

confidence: 99%

Bovine Herpesvirus 1 VP22 Enhances the Efficacy of a DNA Vaccine in Cattle

Zheng

Babiuk

Hurk

2005

J Virol

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For this study, the intercellular trafficking ability of bovine herpesvirus 1 (BHV-1) VP22 was applied to improve the efficacy of a DNA vaccine in calves. A plasmid encoding a truncated version of glycoprotein D (tgD) fused to VP22 was constructed. The plasmid encoding tgD-VP22 elicited significantly enhanced and more balanced immune responses than those induced by a plasmid encoding tgD. Furthermore, protection against a BHV-1 challenge was obtained in calves immunized with the plasmid encoding tgD-VP22, as shown by significant reductions in viral excretion. However, less significant protection was observed for animals vaccinated with the tgD-expressing plasmid, correlating with the lower level of immunity observed prechallenge. This is the first report of the use of VP22 as a transport molecule in the context of a DNA vaccine for a large animal species.

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Priming by DNA immunization augments T-cell responses induced by modified live bovine herpesvirus vaccine

Cited by 22 publications

References 30 publications

Genetic immunisation of cattle against Bovine herpesvirus 1: glycoprotein gD confers higher protection than glycoprotein gC or tegument protein VP8

Genetic immunisation of cattle against Bovine herpesvirus 1: glycoprotein gD confers higher protection than glycoprotein gC or tegument protein VP8

The Immunogenicity and Protective Efficacy of Bovine Herpesvirus 1 Glycoprotein D plus Emulsigen Are Increased by Formulation with CpG Oligodeoxynucleotides

Bovine Herpesvirus 1 VP22 Enhances the Efficacy of a DNA Vaccine in Cattle

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