Priming and Cross-Priming for H-Y in Female Mice

Desquenne-Clark, Lise; Kimura, Hiroyuki; Silvers, Willys K.

doi:10.1097/00007890-199211000-00028

Cited by 7 publications

(3 citation statements)

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“…Immunization of F1(MHC axb ) females with MHC a male cells led to the activation of two sets of CTL: one that could kill MHC a , but not MHC b , male targets, and one with the converse specificity. Other studies, including those from our group, have demonstrated similar results using skin graft models (13,14).…”

supporting

confidence: 78%

CD8 T Cells Specific for a Donor-Derived, Self-Restricted Transplant Antigen Are Nonpathogenic Bystanders after Vascularized Heart Transplantation in Mice

Valujskikh

Zhang

Heeger

2006

The Journal of Immunology

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CD8 T cell cross-priming, an established mechanism of protective antiviral immunity, was originally discovered during studies involving minor transplantation Ags. It is unclear whether or how cross-primed CD8 T cells, reactive to donor-derived, but recipient class I MHC-restricted epitopes, could injure a fully MHC-disparate, vascularized transplant. To address this question we studied host class I MHC-restricted, male transplantation Ag-reactive T cell responses in female recipients of fully MHC-disparate, male heart transplants. Cross-priming to the immune-dominant determinant HYUtyp occurred at low frequency after heart transplantation. CD8 T cell preactivation through immunization with HYUtyp mixed in CFA did not alter the kinetics of acute rejection. Furthermore, neither HYUtyp immunization nor adoptive transfer of HYUtyp-specific TCR-transgenic T cells affected outcome in 1) a model of chronic rejection in the absence of immunosuppression or 2) a model of allograft acceptance induced by costimulatory blockade. The results support the contention that CD8 T cells reactive to host-restricted, but donor-derived, Ags are highly specific and are nonpathogenic bystanders during rejection of MHC-disparate cardiac allografts.

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supporting

confidence: 78%

CD8 T Cells Specific for a Donor-Derived, Self-Restricted Transplant Antigen Are Nonpathogenic Bystanders after Vascularized Heart Transplantation in Mice

Valujskikh

Zhang

Heeger

2006

The Journal of Immunology

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“…Moreover, cross-priming to this epitope has been reported to be inefficient in models when allo (H2 k 3 H2 b ) or ␤ 2 -microglobulin-deficient (␤ 2 m Ϫ/Ϫ ) cells are used as the source of male Ag (16,17). Finally, the HY model is physiologically and pathologically relevant because the minor histocompatibility Ags are normal cellular proteins and have been implicated in the clinical complications associated with graft-versus-host disease.…”

Section: In This Model Cd8mentioning

confidence: 99%

Antigen Persistence Is Required for Dendritic Cell Licensing and CD8+ T Cell Cross-Priming

Jusforgues-Saklani

Uhl

Blachère

et al. 2008

The Journal of Immunology

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It has been demonstrated that CD4+ T cells require Ag persistence to achieve effective priming, whereas CD8+ T cells are on “autopilot” after only a brief exposure. This finding presents a disturbing conundrum as it does not account for situations in which CD8+ T cells require CD4+ T cell help. We used a physiologic in vivo model to study the requirement of Ag persistence for the cross-priming of minor histocompatibility Ag-specific CD8+ T cells. We report inefficient cross-priming in situations in which male cells are rapidly cleared. Strikingly, the failure to achieve robust CD8+ T cell activation is not due to a problem with cross-presentation. In fact, by providing “extra help” in the form of dendritic cells (DCs) loaded with MHC class II peptide, it was possible to achieve robust activation of CD8+ T cells. Our data suggest that the “licensing” of cross-presenting DCs does not occur during their initial encounter with CD4+ T cells, thus accounting for the requirement for Ag persistence and suggesting that DCs make multiple interactions with CD8+ T cells during the priming phase. These findings imply that long-lived Ag is critical for efficient vaccination protocols in which the CD8+ T cell response is helper-dependent.

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“…Regarding cross-priming with respect to the skin isograft model, a previous study by Desquenne-Clark et al [31] revealed that although female mice (H-2 b and H-2 g ) that are responders to H-Y can be crossprimed for H-Y antigen, the ability to do so varies from strain to strain and can be influenced by whether they are exposed to H-Y via a subcutaneous hind footpad inoculation of male cells or via a male skin graft. Unlike responder strains, low-responder females (H-2 k ) to H-Y can only be sensitized to male skin isografts following a hind footpad inoculation of syngeneic male cells, not by allogeneic male cells.…”

Section: Discussionmentioning

confidence: 99%

Immunological behavior of enhanced green fluorescent protein (EGFP) as a minor histocomaptibility antigen with a special reference to skin isograft and specific regulation of local graft-versus-host reaction (GvHR)

et al. 2009

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Priming and Cross-Priming for H-Y in Female Mice

Cited by 7 publications

References 0 publications

CD8 T Cells Specific for a Donor-Derived, Self-Restricted Transplant Antigen Are Nonpathogenic Bystanders after Vascularized Heart Transplantation in Mice

CD8 T Cells Specific for a Donor-Derived, Self-Restricted Transplant Antigen Are Nonpathogenic Bystanders after Vascularized Heart Transplantation in Mice

Antigen Persistence Is Required for Dendritic Cell Licensing and CD8+ T Cell Cross-Priming

Immunological behavior of enhanced green fluorescent protein (EGFP) as a minor histocomaptibility antigen with a special reference to skin isograft and specific regulation of local graft-versus-host reaction (GvHR)

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