Primers for amplifying the hypervariable, male‐transmitted <i>COII‐COI</i> junction region in amblemine freshwater mussels (Bivalvia: Unionoidea: Ambleminae)

Walker, Jennifer; Bogan, Arthur E.; Bonfiglio, Elsie A.; Campbell, David; Christian, Alan D.; Curole, Jason P.; Harris, John L.; Wojtecki, Rudy J.; Hoeh, Walter R.

doi:10.1111/j.1471-8286.2006.01630.x

Cited by 45 publications

(40 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…PCR conditions for both markers, the female lineages of mitochondrial cytochrome c oxidase subunit 1, COI (LCO22me2 + HCO700dy2; Walker et al, 2006Walker et al, , 2007andLCO1490 + HCO2198;Folmer et al, 1994) and 28S ribosomal RNA (28S-RD1.3f and 28S-rD4b; Whiting, 2002) were described in Froufe et al (2014). Annealing temperatures of 48°C were used for COI (LCO1490 + HCO2198) and 28S; and 50°C for COI (LCO22me2 + HCO700dy2).…”

Section: Dna Extraction Amplification and Sequencingmentioning

confidence: 99%

Phylogeny of the most species-rich freshwater bivalve family (Bivalvia: Unionida: Unionidae): Defining modern subfamilies and tribes

Lopes‐Lima

Froufe

et al. 2017

Molecular Phylogenetics and Evolution

Self Cite

136

130

View full text Add to dashboard Cite

a b s t r a c t Freshwater mussels of the order Unionida are key elements of freshwater habitats and are responsible for important ecological functions and services. Unfortunately, these bivalves are among the most threatened freshwater taxa in the world. However, conservation planning and management are hindered by taxonomic problems and a lack of detailed ecological data. This highlights the urgent need for advances in the areas of systematics and evolutionary relationships within the Unionida. This study presents the most comprehensive phylogeny to date of the larger Unionida family, i.e., the Unionidae. The phylogeny is based on a combined dataset of 1032 bp (COI + 28S) of 70 species in 46 genera, with 7 of this genera being sequenced for the first time. The resulting phylogeny divided the Unionidae into 6 supported subfamilies and 18 tribes, three of which are here named for the first time (i.e., Chamberlainiini nomen novum, Cristariini nomen novum and Lanceolariini nomen novum). Molecular analyses were complemented by investigations of selected morphological, anatomical and behavioral characters used in traditional phylogenetic studies. No single morphological, anatomical or behavioral character was diagnostic at the subfamily level and few were useful at the tribe level. However, within subfamilies, many tribes can be recognized based on a subset of these characters. The geographical distribution of each of the subfamilies and tribes is also presented. The present study provides important advances in the systematics of these extraordinary taxa with implications for future ecological and conservation studies.

show abstract

Section: Dna Extraction Amplification and Sequencingmentioning

confidence: 99%

Phylogeny of the most species-rich freshwater bivalve family (Bivalvia: Unionida: Unionidae): Defining modern subfamilies and tribes

Lopes‐Lima

Froufe

et al. 2017

Molecular Phylogenetics and Evolution

Self Cite

136

130

View full text Add to dashboard Cite

show abstract

“…Whole genomic DNA was extracted from small tissue pieces preserved in 96% ethanol, using a standard high-salt protocol (Sambrook et al, 1989). A fragment of ~700 bp of CO1 gene was amplified by PCR using the LCO22me2 and HCO700dy2 primers (Walker et al, 2006(Walker et al, , 2007. The PCR conditions (25 μL reactions) are described elsewhere (Froufe et al, 2014), with the annealing temperature being done at 50°C.…”

Section: Dna Extraction Amplification and Sequencingmentioning

confidence: 99%

When and how? Freshwater mussel recolonization in Lake Orta

et al. 2016

View full text Add to dashboard Cite

Thanks to a video posted on a social network, live mussels of Unio elongatulus, have been recorded from Lake Orta (Italy) over one century after the last (and only) report. With its long and well documented history of pollution, Lake Orta offers the opportunity to document the post-extirpation recovery of freshwater unionid mussels. This case evidences that recovery/recolonization is possible despite a high devastation in the past, and permits to evaluate how fast recolonization may occur, in which way, and in what conditions. The answer to the how fast was sought by estimating the age of the larger and seemingly older individuals of the population. To address the in which way we compared the haplotypes of Lake Orta specimens of Unio elongatulus (the only species present) with those of surrounding populations. We concluded that, since Lake Orta lacks a direct connection with the putative source populations, colonizing mussels were almost certainly transported by fish carrying glochidia that were used for lake restocking after liming. Data from the long-term monitoring of water chemistry and sediments have allowed defining what conditions proved to be suitable for survival making possible the start of mussels recovery. But not only water and sediment quality matters for mussels recovery, which was delayed by nearly ten years after the reappearance of fish. This delay reflects the need of the whole trophic chain to be reestablished to allow the survival of the suitable and healthy host-fish populations necessary for mussels reproduction.

show abstract

“…500 bp fragment), with 16SL and 16SH primers (Palumbi et al, 1991); the F-type mtDNA cytochrome oxidase subunit 1 gene (COI; ca. 700 bp fragment), with LCO_22me and HCO_700dy primers (Walker et al, 2006(Walker et al, , 2007; and for the nuclear 28Sr DNA (ca. 800 bp fragment) with 28S-RD1.3f and 28S-rD4b primers (Whiting, 2002).…”

Section: Sequencing Alignment Phylogenetic Analysesmentioning

confidence: 99%

Phylogeny, phylogeography, and evolution in the Mediterranean region: News from a freshwater mussel (Potomida, Unionida)

Froufe

Prié

Faria

et al. 2016

Molecular Phylogenetics and Evolution

View full text Add to dashboard Cite

a b s t r a c tThe Potomida genus (Bivalvia, Unionida) has a Circum-Mediterranean distribution and like other freshwater mussel species, its populations have suffered dramatic declines. Although this genus is currently considered as monotypic, it has a long history of taxonomic revisions and presently many aspects of its systematics and evolutionary history are unclear. We sampled a total of 323 individuals from 39 different sites across the Potomida genus distribution, and sequenced two mitochondrial (16S rDNA and Cytochrome c Oxidase Subunit I) and one nuclear (28S rDNA) genes to clarify its phylogeny and phylogeographic history. Our results show that the genus includes two well-supported clades, one comprising solely the western Mediterranean species Potomida littoralis, and the other including two eastern Mediterranean species, the Greek endemic P. acarnanica and the Anatolian and Middle Eastern P. semirugata. We suggest that Potomida started radiating during the upper Miocene, and that both vicariance and dispersal events shaped the diversification and distribution of the genus along the Mediterranean region. P. littoralis is further divided in two mitochondrial lineages, one restricted to Europe and the other occurring mostly in North Africa. Moreover, some European basins present both lineages in sympatry. The conservation status of the three recognized species should be reevaluated, particularly P. acarnanica, since it is restricted to two Greek river basins presenting a high risk of extinction. Overall, our results clarify some important gaps in knowledge concerning the phylogeny, phylogeography and evolution of the Potomida genus in the Mediterranean region with important taxonomical, ecological and conservational implications.

show abstract

Primers for amplifying the hypervariable, male‐transmitted COII‐COI junction region in amblemine freshwater mussels (Bivalvia: Unionoidea: Ambleminae)

Cited by 45 publications

References 6 publications

Phylogeny of the most species-rich freshwater bivalve family (Bivalvia: Unionida: Unionidae): Defining modern subfamilies and tribes

Phylogeny of the most species-rich freshwater bivalve family (Bivalvia: Unionida: Unionidae): Defining modern subfamilies and tribes

When and how? Freshwater mussel recolonization in Lake Orta

Phylogeny, phylogeography, and evolution in the Mediterranean region: News from a freshwater mussel (Potomida, Unionida)

Contact Info

Product

Resources

About