Primer reporte de Tomato brown rugose fruit virus (ToBRFV) en Michoacán, México

Abstract

A nivel mundial, la producción de jitomate y chile es de gran relevancia. Recientemente, en Israel y Jordania se notificó la presencia de un nuevo miembro del género Tobamovirus en plantaciones de jitomate, al cual se le llamó Tomato brown rugose fruit virus (ToBRFV). Los frutos de plantas infectadas manifiestan áreas amarillas, necróticas o de color marrón incluso rugosidades. En los municipios de Yurécuaro y Tanhuato, Michoacán, se han observado cultivos de jitomate y chile con síntomas similares a los antes mencionados por lo que, el objetivo del presente trabajo fue determinar si el ToBRFV se encuentra presente en estas localidades. Se colectó tejido foliar de jitomate con síntomas y se realizó RT-PCR con iniciadores que amplifican un segmento del ORF2 del genoma de este virus; además, se prepararon rejillas para su observación al microscopio electrónico de transmisión (MET). Se obtuvo el amplicón esperado y las secuencias tuvieron una similitud de 99 a 100 % con ToBRFV. Al MET se observaron partículas virales en forma de varilla rígida típicas de tobamovirus. Hasta donde sabemos este es el primer reporte de la presencia del ToBRFV asociado a plantas de jitomate y chile cultivados en México.

Development and evaluation of a loop-mediated isothermal amplification (LAMP) assay for the detection ofTomato brown rugose fruit virus(ToBRFV)

“…Tomato brown rugose fruit virus (ToBRFV) is a member of the Tobamovirus genus with a host range including tomato, pepper and some weeds such as nightshades [3]. It was first detected in Israel in 2014 [4] and later in countries in North America [5,6], Asia [7–9] and Europe [10,11]. Within North America, both the United States [12] and Mexico [13] consider ToBRFV to be a regulated pest.…”

Tomato brown rugose fruit disease: current distribution, knowledge and future prospects

“…To date, the incidence and current distribution of ToBRFV infection have been reported from Jordan (Salem et al , ), Israel (Luria et al , ), Mexico (Cambrón‐Crisantos et al , ), the USA (Chitambar, ; Ling et al , ), Germany (Menzel et al , ), Italy (Panno et al , ), Palestine (Alkowni et al , ), Turkey (Fidan et al , ) and China (Yan et al , ). Likely occurrences have also been reported but not confirmed in Chile, Ethiopia, Sudan and the Netherlands.…”

“…Symptoms expressed on plants consisted of mild to severe mosaic, narrow leaves and yellow spotted fruits, similar to those induced by the tobamoviruses TMV and tomato mosaic virus (ToMV), but following sequence analysis the virus was found to be an isolate of ToBFRV. More recently, ToBRFV was detected in tomato and chilli pepper plants growing in nurseries in Yurecuaro, Michoacan, Mexico (Cambrón‐Crisantos et al , ), although details of the incidence were not provided.…”

Development and evaluation of a loop-mediated isothermal amplification (LAMP) assay for the detection of Tomato brown rugose fruit virus (ToBRFV)

“…Tomato brown rugose fruit virus (ToBRFV) is a member of the Tobamovirus genus with a host range including tomato, pepper and some weeds such as nightshades [4]. It was first detected in Israel in 2014 [5] and later in countries in North America [6,7], Asia [8][9][10] and Europe [11,12]. Within North America, both the United States [13] and Mexico [14] consider ToBRFV to be a regulated pest.…”