Primer Design for the Identification of Oral Rothia Species Using Multiplex PCR

Abstract

Rothia dentocariosa and Rothia mucilaginosa, which are opportunistic pathogens that are capable of causing serious infections, inhabit the oral cavity. However, there is no suitable method for assessing the prevalence of Rothia species in the oral cavity. In this study, four polymerase chain reaction(PCR)primers were designed based on partial sequences of the 16S rDNA genes of the abovementioned oral Rothia species. These primers react to the oral Rothia species and did not react to other Rothia species except Rothia aeria. Moreover, representative non-Rothia oral bacteria displayed negative reactions to these primers.These results indicate that these primers are useful for identifying R. dentocariosa and R. mucilaginosa. We also developed a simple multiplex PCR procedure involving the two primer pairs designed in the present study as a rapid and reliable method of identifying known oral Rothia species.

New Primer Design for Identification of Oral rothia, Including R. aeria, Using Multiplex PCR

“…The design of species-specific primers for R. dentocariosa, R. mucilaginosa and R. aeria was performed as described previously (21) . Briefly, the 16S rRNA sequences of R. aeria…”

“…Thus, a simple and more reliable assay for identifying Rothia species is required. In our previous study, we designed speciesspecific primers for detecting R. dentocariosa and R. mucilaginosa using a multiplex PCR method (21) . However, this method was unable to distinguish R. dentocariosa from R. aeria.…”