Primer design for identifying economically important <i><scp>L</scp>iriomyza</i> species (<scp>D</scp>iptera: <scp>A</scp>gromyzidae) by multiplex <scp>PCR</scp>

Nakamura, Shigeo; Masuda, Toshio; Konishi, Kazuhiko; Tokumaru, Susumu; Ueno, Kazuto; Yamaguchi, Takuhiro

doi:10.1111/1755-0998.12025

Cited by 28 publications

(25 citation statements)

References 34 publications

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“…To reduce the possibility of taxonomic confusion, rapid, reliable, sensitive and simple molecular identification methods based on real-time polymerase chain reactions and enzyme-linked immunosorbent assays (ELISA) have been developed. 56,57 Furthermore, rapid bioassay techniques to detect insecticide resistance have been developed so that changes in resistance status can be monitored in a timely manner. 58 Taking advantage of such methods and understanding factors that give rise to leafminer outbreaks will be useful in managing this pest in China.…”

Section: Lessons Learned and Conclusionmentioning

confidence: 99%

A decade of leafminer invasion in China: lessons learned

Gao

Reitz

Xing

et al. 2017

Pest Management Science

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Liriomyza trifolii (Burgess) is a highly invasive species that has become established in agricultural and ornamental crops throughout the world. L. trifolii was first recorded in China in 2005 in Guangdong Province. Subsequently, its known distribution in China has rapidly expanded to another 11 provinces (Zhejiang, Jiangsu, Shanghai, Fujian, Guangxi, Shandong, Hainan, Hebei, Henan, Hubei and Anhui), where it is currently causing considerable damage and economic losses. Experimental research and field surveys have contributed to our understanding of the ecology of L. trifolii and particularly the process of invasion and its interactions with other established, exotic Liriomyza leafminer species. A number of factors have contributed to the successful establishment of L. trifolii and displacement of interspecific competitors. In China, L. trifolii has become a particularly devastating pest in areas with intensive farming with extensive monocultures and a reliance on insecticide use. Integrated pest management incorporating judicious applications of insecticide combined with biological and cultural controls is likely to provide the best long-term management strategy for L. trifolii. We provide information regarding 10 years of L. trifolii invasion in China and discuss areas of future research to enhance our overall understanding of the biology and ecology of L. trifolii and to improve management programmes for this widespread invasive insect pest. © 2017 Society of Chemical Industry.

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Section: Lessons Learned and Conclusionmentioning

confidence: 99%

A decade of leafminer invasion in China: lessons learned

Gao

Reitz

Xing

et al. 2017

Pest Management Science

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“…() ( n = 40), Nakamura et al . () ( n = 5) and Blacket et al . () ( n = 34) (see Supporting information, Table S1).…”

Section: Methodsmentioning

confidence: 88%

“…International trade of ornamental and vegetable crops facilitates their dispersion because the eggs and larval mines are not always visible in the host. In addition, the late identification (in some cases erroneous) and the occurrence of cryptic species among leafminer flies hinder strategic planning for the elimination and/or containment of these invasive pest species (Parrella, ; Scheffer et al ., ; Gao et al ., ; Nakamura et al ., ).…”

Section: Introductionmentioning

confidence: 97%

“…Leafminer flies are quarantine pests in several countries. However, these pest species continue to be introduced and reintroduced in several regions of the world (Abe & Tokumaru, ; Nakamura et al ., ; Blacket et al ., ). International trade of ornamental and vegetable crops facilitates their dispersion because the eggs and larval mines are not always visible in the host.…”

Section: Introductionmentioning

confidence: 98%

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Host range and genetic strains of leafminer flies (Diptera: Agromyzidae) in eastern Brazil reveal a new divergent clade ofLiriomyza sativae

et al. 2016

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1 Leafminer flies are phytophagous and cosmopolitan pests. Leafminer fly outbreaks and damage to cultivated plants have steadily increased in several regions in the world. 2 In the present study, we report host range, geographical distribution, Wolbachia infection and mitochondrial strains of leafminer fly species from eastern Brazil. 3 Four leaf miner fly species were identified using cytochrome c oxidase subunit I DNA barcoding. Liriomyza sativae is the main pest as a result of its high polyphagy and wide distribution. Liriomyza brassicae, Liriomyza huidobrensis and Calycomyza malvae are important in Brassicaceae, Curcubitaceae and Malvaceae crops, respectively. 4 There is no relationship among host range, geographical distribution and mitochondrial strains of leafminer fly species. We did not find Wolbachia infection in any of the specimens collected. 5 Phylogeographical analyses suggests that there are four strains of L. sativae in the world, one of which is endemic from Brazil. The lack of shared haplotypes between Brazilian specimens and those from other world regions indicates the absence of recent gene flow of leafminer flies from Brazil with specimens from Americas and Old world. The exception is L. brassicae, which exhibits one haplotype shared among Brazil, Philippines and Sri Lanka. 6 The host range identification and geographical isolation of leafminer fly species from Brazil comprise useful information for quarantine and pest management purposes.

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“…Recently, multiplex PCR assays were developed for differentiating Liriomyza species (Miura et al ; Guan et al ; Nakamura et al ). This method required amplification of a region of mitochondrial (COI or COII) or nuclear gene (ITS) regions using multiprimer sets which could differentiate Liriomyza species by the numbers and sizes of the fragments amplified.…”

Section: Introductionmentioning

confidence: 99%

Multiplex real‐time PCR assay for the detection of three invasive leafminer species: Liriomyza huidobrensis, L. sativae and L. trifolii (Diptera: Agromyzidae)

Sooda

Gunawardana

et al. 2016

Austral Entomology

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Rapid and precise identification of immature stages of leafminers of the genus Liriomyza Mik associated with imported fresh produce is essential to ensure appropriate biosecurity decisions at the border, in quarantine and post border. The leafminers Liriomyza huidobrensis, Liriomyza sativae and Liriomyza trifolii are not present in New Zealand and classified as regulated pests when detected at New Zealand's border. To assist rapid species identification of the immature stages of interceptions, a multiplex real-time TaqMan PCR assay was developed to identify these three species simultaneously in a single test. Species-specific primers and probes were designed by amplifying the mitochondrial COI gene of each targeting species, respectively. The multiplex real-time PCR assay demonstrated high specificity for all three target species and the assay detected DNA quantities as low as 0.1 pg for all species. Linear responses and high correlation coefficients between the amount of DNA and C q values for each species were also achieved. Therefore, the assay demonstrated its sensitivity and reliability for the identification of these three invasive Liriomyza species.

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Primer design for identifying economically important Liriomyza species (Diptera: Agromyzidae) by multiplex PCR

Cited by 28 publications

References 34 publications

A decade of leafminer invasion in China: lessons learned

A decade of leafminer invasion in China: lessons learned

Host range and genetic strains of leafminer flies (Diptera: Agromyzidae) in eastern Brazil reveal a new divergent clade ofLiriomyza sativae

Multiplex real‐time PCR assay for the detection of three invasive leafminer species: Liriomyza huidobrensis, L. sativae and L. trifolii (Diptera: Agromyzidae)

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