Primer Design and Optimization of Annealing Temperature for Gene Amplification GSTL2 on Rice
Violita Violita,
Afifatul Achyar,
Zulyusri Zulyusri
et al.
Abstract:Glutathione S-Transferase is a superfamily enzyme that has many roles for living things, especially in the detoxification of Reactive Oxygen Species (ROS), one of which is rice plants. One gene of this family that has a role for plants is GSTL2 This gene is known to have a contribution to Arabidopsis and Oryza sativa L. against abiotic stress. To find out how this gene expression is requires a primer to specifically amplify this gene, and an optimal annealing temperature to support the success of the primer. T… Show more
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