Primed polymorphonuclear leukocytes constitute a possible link between inflammation and oxidative stress in hyperlipidemic patients

Mazor, Rafi; Shurtz-Swirski, Revital; Farah, Raymond; Kristal, Batya; Shapiro, Galina; Dorlechter, Faina; Cohen-Mazor, Meital; Meilin, Edna; Snitkovski, Tamara; Sela, Shifra

doi:10.1016/j.atherosclerosis.2007.08.014

Cited by 87 publications

(72 citation statements)

References 21 publications

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“…Our results support previous studies on the pathogenic role of primed neutrophils [1,[8][9][10][11][12][13][14] where it has been shown that minimally-primed neutrophils are able to induce overexpression of E-Selectin, VCAM-1 and ICAM-1, thus promoting an activating phenotype of EC. However, several novel observations were made here.…”

Section: Discussionsupporting

confidence: 82%

“…Secondly, minimally-primed dHL-60 cells neither alter EC viability nor induce irreversible lethal events that would result in cell detachment and apoptosis, outcomes reported when highly primed PMN are used [10,13,14]. This is coherent with the threshold degree of priming employed here, at variance from that used in previously published studies.…”

Section: Page 13 Of 24contrasting

confidence: 55%

“…A c c e p t e d M a n u s c r i p t 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 13 First, minimally-primed neutrophil surrogates can activate EC as long as they are co-cultured for a time period considerably higher (16 h) than that reported in studies employing highlyprimed neutrophils from hemodialysis, diabetic or hyperlipidemic patients (15 min) [10,13,14]. Secondly, minimally-primed dHL-60 cells neither alter EC viability nor induce irreversible lethal events that would result in cell detachment and apoptosis, outcomes reported when highly primed PMN are used [10,13,14].…”

Section: Page 13 Of 24mentioning

confidence: 68%

“…For instance, PMNs from patients with risk of developing atherosclerosis and cardiovascular complications (e.g. essential hypertension, diabetes mellitus type II or chronic renal failure) display a certain degree of priming that correlates positively with both the magnitude and type of the disease, leading to an oxidative imbalance within the endothelium [8][9][10][11][12][13][14]. This has led to propose that loose -but continuous -interaction between primed neutrophils and EC may induce a chronic threshold injury to the endothelium and a systemic oxidative stress able to initiate atherosclerosis and cardiovascular complications [8][9][10].…”

Section: Introductionmentioning

confidence: 99%

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Cross-talk between minimally primed HL-60 cells and resting HUVEC reveals a crucial role for adhesion over extracellularly released oxidants

Allegra

D’Acquisto

Tesoriere

et al. 2011

Biochemical Pharmacology

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To cite this version:M. Allegra, F.D'Acquisto, L. Tesoriere, M.A. Livrea, M. Perretti. Cross-talk between minimallyprimed HL-60 cells and resting HUVEC reveals a crucial role for adhesion over extracellularly-released oxidants.. Biochemical Pharmacology, Elsevier, 2010, 81 (3) This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. A c c e p t e d M a n u s c r i p t 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 2 AbstractThis study demonstrates that a long-lasting co-culture of neutrophil surrogates (HL-60 cells), minimally-primed by platelet activating factor (PAF), and resting endothelial cells (EC) results in the elaboration of an hyper-adhesive endothelial surface, as measured by the increase in the expression of endothelial adhesion molecules E-Selectin, VCAM-1, and ICAM-1. This endothelial dysfunction is mediated by the activation of the redox-sensitive transcription factor NF-B through an exclusive adhesion-driven mechanism active in the endothelial cell: reactive oxygen and nitrogen species, extracellularly-released by minimallyprimed HL-60 cells, are not involved in the induction of the endothelial dysfunction.By exploring for the first time the potential for minimally-primed neutrophil surrogates to induce endothelial dysfunction, this study suggests a novel mechanism which may be operative in pathologies, mediated by minimally-primed neutrophils, such as hyperdyslipidemia and cardiovascular complications.

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Section: Discussionsupporting

confidence: 82%

Section: Page 13 Of 24contrasting

confidence: 55%

Section: Page 13 Of 24mentioning

confidence: 68%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Cross-talk between minimally primed HL-60 cells and resting HUVEC reveals a crucial role for adhesion over extracellularly released oxidants

Allegra

D’Acquisto

Tesoriere

et al. 2011

Biochemical Pharmacology

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“…Under chronic inflammatory conditions, such as liver disease, hypertension, smoking and renal failure 29 neutrophil granulocytes can be primed, which makes them more ready to full activation upon a second stimulus. In our study cohort neutrophil priming, oxidative burst upon stimulation and phagocytosis was not different to normal controls and hyperlipidemia was not associated with changes in neutrophil function.…”

Section: Discussionmentioning

confidence: 99%

The influence of probiotic supplementation on gut permeability in patients with metabolic syndrome: an open label, randomized pilot study

et al. 2012

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BACKGROUND/OBJECTIVES: Obesity and metabolic disorders are linked to inflammation via gut microbiota and/or gut permeability. Gut-derived endotoxin triggers inflammation leading to metabolic syndrome (MetS) and contributing to oxidative stress. We intended to investigate the effect of Lactobacillus casei Shirota on gut permeability, presence of endotoxin and neutrophil function in MetS. SUBJECTS/METHODS: Patients with MetS were randomized to receive 3 Â 6.5 Â 10 9 CFU L. casei Shirota (probiotic group) or not for 3 months. Gut permeability was assessed by a differential sugar absorption method and by determination of diaminooxidase serum levels, endotoxin by an adapted limulus amoebocyte lysate assay, neutrophil function and toll-like receptor (TLR) expression by flow cytometry and ELISA was used to detect lipopolysaccharide-binding protein (LBP) and soluble CD14 (sCD14) levels. RESULTS: Twenty-eight patients and 10 healthy controls were included. Gut permeability was significantly increased in MetS compared with controls but did not differ between patient groups. None of the patients were positive for endotoxin. LBP and sCD14 levels were not significantly different from healthy controls. High-sensitive C-reactive protein and LBP levels slightly but significantly increased after 3 months within the probiotics group. Neutrophil function and TLR expression did not differ from healthy controls or within the patient groups. CONCLUSIONS: Gut permeability of MetS patients was increased significantly compared with healthy controls. L. casei Shirota administration in the MetS patients did not have any influence on any parameter tested possibly due to too-short study duration or underdosing of L. casei Shirota.

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Anti–apolipoprotein A‐1 IgG predicts major cardiovascular events in patients with rheumatoid arthritis

Vuilleumier¹,

Bas²,

Pagano³

et al. 2010

Arthritis & Rheumatism

163

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Objective. To determine whether antiapolipoprotein A-1 (anti-Apo A-1) IgG are associated with major cardiovascular events in patients with rheumatoid arthritis (RA).Methods. We determined anti-Apo A-1 IgG levels and the concentrations of cytokines, oxidized lowdensity lipoprotein (LDL), and matrix metalloproteinase 1 (MMP-1) MMP-2, MMP-3, and MMP-9 in sera from 133 patients with RA who did not have cardiovascular disease at baseline, all of whom were longitudinally followed up over a median period of 9 years. A major cardiovascular event was defined as a fatal or nonfatal stroke or acute coronary syndrome. The proinflammatory effects of anti-Apo A-1 IgG were assessed on human macrophages in vitro.Results. During followup, the overall incidence of major cardiovascular events was 15% (20 of 133 patients). At baseline, anti-Apo A-1 IgG positivity was 17% and was associated with a higher incidence of major cardiovascular events (adjusted hazard ratio 4.2, 95% confidence interval 1.5-12.1). Patients who experienced a subsequent major cardiovascular event had higher circulating levels of anti-Apo A-1 IgG at baseline compared with those who did not have a major cardiovascular event. Receiver operating curve analysis showed that anti-Apo A-1 IgG was the strongest of all tested biomarkers for the prediction of a subsequent major cardiovascular event, with an area under the curve value of 0.73 (P ‫؍‬ 0.0008). At the predefined and previously validated cutoff levels, the specificity and sensitivity of anti-Apo A-1 IgG to predict major cardiovascular events were 50% and 90%, respectively. Anti-Apo A-1 IgG positivity was associated with higher median circulating levels of interleukin-8 (IL-8), oxidized LDL, and MMP-9 and higher proMMP-9 activity as assessed by zymography. On human macrophages, anti-Apo A-1 IgG induced a significant dose-dependent increase in IL-8 and MMP-9 levels and proMMP-9 activity.Conclusion. Anti-Apo A-1 IgG is an independent predictor of major cardiovascular events in RA, possibly by affecting vulnerability to atherosclerotic plaque.

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Primed polymorphonuclear leukocytes constitute a possible link between inflammation and oxidative stress in hyperlipidemic patients

Cited by 87 publications

References 21 publications

Cross-talk between minimally primed HL-60 cells and resting HUVEC reveals a crucial role for adhesion over extracellularly released oxidants

Cross-talk between minimally primed HL-60 cells and resting HUVEC reveals a crucial role for adhesion over extracellularly released oxidants

The influence of probiotic supplementation on gut permeability in patients with metabolic syndrome: an open label, randomized pilot study

Anti–apolipoprotein A‐1 IgG predicts major cardiovascular events in patients with rheumatoid arthritis

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