Primatone RL in mammalian cell culture media

Mizrahi, A.

doi:10.1002/bit.260191013

Cited by 38 publications

(8 citation statements)

References 8 publications

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“…In addition, however, proteomics can be used to examine the purity and effectiveness of raw materials used in the fermentation process. It has been noted that materials such as primatone are often poorly defined complex biological samples (16). In this context, one issue about the production of biologic pharmaceuticals that is particularly troubling is the inability for a process development scientist to effectively assay the effectiveness of different media supplements.…”

Section: Discussionmentioning

confidence: 99%

Proteomic Analysis for the Assessment of Different Lots of Fetal Bovine Serum as a Raw Material for Cell Culture. Part IV. Application of Proteomics to the Manufacture of Biological Drugs

Zheng

Baker

Hancock

et al. 2006

Biotechnology Progress

273

229

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Fetal bovine serum (FBS) is the most widely used growth supplement for cell cultures, primarily because of its high levels of growth stimulatory factors and low levels of growth inhibitory factors. Maintaining successful and consistent cell fermentations can be difficult, as FBS is a complex natural product and may vary from lot to lot even from a single manufacturer. The quality and concentration of both bulk and specific proteins can affect cell growth. Quality control tools for FBS are relatively primitive and expensive given the complexity of the sample and the large amounts of FBS used. We undertook this study to examine whether proteomics could be used as a tool to analyze the variability of different fermentation processes. We hypothesized that inconsistent cell growth in fermentations could be due to the quality of FBS and that different lots of FBS had varying concentrations of proteins such as growth stimulatory factors, growth inhibitory factors, and/or other proteins that may correlate with cellular growth rate. To investigate whether this was the case, we grew three batches of adult retinal pigment epithelial cells (ARPE-19) using three different lots of fetal bovine serum (FBS-Ia, FBS-Ib, and FBS-II). We found that the growth rate of the culture was significantly and consistently higher in the FBS-II lot. To determine why the other lots promoted different growth properties, we used proteomic techniques to analyze the protein composition of the three lots. We then performed a time course study to monitor specific changes in individual proteins in the fermentation medium. The amount of several extracellular matrix and structural proteins, which are indicators of cell growth, increased over time. Alternatively, components supplied by the FBS addition, such as nutritional-related and cell-spreading-related proteins, decreased over time.

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Section: Discussionmentioning

confidence: 99%

Proteomic Analysis for the Assessment of Different Lots of Fetal Bovine Serum as a Raw Material for Cell Culture. Part IV. Application of Proteomics to the Manufacture of Biological Drugs

Zheng

Baker

Hancock

et al. 2006

Biotechnology Progress

273

229

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“…On day 10, cells were passed to fresh confluent OP9 layers with a-MEM/20% FCS/3% SCF-CM/2% Flt-3L-CM at 7 · 10 4 cells/mL in T25 flask. On day 12, the media was changed to Iscove's modified Dulbecco's medium (IMDM)/2% FCS/ 0.03% Primatone [35]/1% IL-7-CM/2% Flt-3L-CM. On day 15, all cells were tapped off, filtrated, and subsequently passed to fresh OP9 layers (70% confluency).…”

Section: Differentiation Of Es and Ips Cellsmentioning

confidence: 99%

Induced Pluripotent Stem Cells Expressing Elevated Levels of Sox-2, Oct-4, and Klf-4 Are Severely Reduced in Their Differentiation from Mesodermal to Hematopoietic Progenitor Cells

Seiler

Noghabi

Karjalainen

et al. 2011

Stem Cells and Development

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Induced pluripotent stem (iPS) cells have been generated from bone marrow (BM) hematopoietic progenitor cells by ectopic expression of Sox-2, Oct-4, and Klf-4 with the hope that they may differentiate more efficiently than embryonic stem (ES) cells in vitro into hematopoietic cell lineages because of their epigenetic memory. An in vitro culture system has been standardized to allow a quantitative assessment of the capacities of different ES, BM-derived iPS, and fibroblast-derived iPS cell lines developing to erythroid, myeloid, and lymphoid cell lineages. Surprisingly, the efficiency to differentiate BM-derived iPS cells to hematopoietic cells in vitro is severely reduced compared with ES cells and fibroblast-derived iPS cells. Undifferentiated as well as differentiated stages of the BM-derived iPS lines express elevated mRNA levels of the transcription factors Sox-2, Oct-4, and Klf-4 with which the iPS cells have been transduced. Overexpression of the transcription factors inhibits development of Flk-1(+) mesodermal to CD45(+) hematopoietic progenitors. The overexpression of Sox-2 appears to be inversely related to hematogenic potency. These results suggest that iPS cell generation with the aim of developing hematopoietic cells should be controlled and selected for low levels of transduced Sox-2, Oct-4, and Kfl-4 expression.

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“…This article reports the effects of a peptone, Primatone RL (Mizrahi, 1977), upon the sialylation of human interferon-␥ (IFN-␥) derived from both batch and fed-batch CHO cell cultures. IFN-␥ is a cytokine which exhibits antiviral, antiproliferative, and immunomodulatory activities (Farrar and Schreiber, 1993).…”

Section: Introductionmentioning

confidence: 99%

Influence of Primatone RL supplementation on sialylation of recombinant human interferon-γ produced by Chinese hamster ovary cell culture using serum-free media

Xie²,

Harmon³

et al. 1997

Biotechnol. Bioeng.

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Primatone RL in mammalian cell culture media

Cited by 38 publications

References 8 publications

Proteomic Analysis for the Assessment of Different Lots of Fetal Bovine Serum as a Raw Material for Cell Culture. Part IV. Application of Proteomics to the Manufacture of Biological Drugs

Proteomic Analysis for the Assessment of Different Lots of Fetal Bovine Serum as a Raw Material for Cell Culture. Part IV. Application of Proteomics to the Manufacture of Biological Drugs

Induced Pluripotent Stem Cells Expressing Elevated Levels of Sox-2, Oct-4, and Klf-4 Are Severely Reduced in Their Differentiation from Mesodermal to Hematopoietic Progenitor Cells

Influence of Primatone RL supplementation on sialylation of recombinant human interferon-γ produced by Chinese hamster ovary cell culture using serum-free media

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