Primary structure of the α‐subunit of bovine adenylate cyclase‐inhibiting G‐protein deduced from the cDNA sequence

Abstract

The primary structure of the a-subunit of the adenylate cyclase-inhibiting G-protein (G,) has been deduced from the nucleotide sequence of cloned DNA complementary to the bovine cerebral mRNA encoding the polypeptide. A much higher degree of amino acid sequence homology is observed between the ix-subunits of G, and transducin (68%) than between those of G, and the adenylate cyclase-stimulating G-protein (G,) (43%) or between those of transucin and G, (42%

Localization of mRNAs encoding the α‐subunits of signal‐transducing G‐proteins within rat brain and among peripheral tissues

“…(ii) Using stringencies of hybridization identical to those used for in situ hybridizations, all of the probes identified mRNA species on Northern blots with the same size as previously reported using 196 long cloned probes [14][15][16][17][18]24,25,27,28]. We suggest that the additional, larger, mRNA species observed with the G, and G, probes may represent unprocessed RNA transcripts.…”

“…We call the bovine (241 and human [28] Gi sequences Gil. These sequences, obtained from brain cDNA libraries, agree precisely with the chemically determined sequence of Gi from rat [ 131 and bovine [24] brain. We term the Gi cloned from rat C6 glioma [ 131, mouse macrophage [ 141 and human monocyte [25] libraries Gi2.…”

“…The sequences of rat [ 131, mouse [ 141, human [15,16], and bovine G, [17,18]; rat [13] and bovine G, [19]; bovine rod [20-221 and cone [23] transducins; and bovine [24], human [25], rat [ 131 and mouse [14] Gi have been reported. For Go and G, the protein sequences predicted from cDNAs are almost completely conserved across species.…”

Characterization of four GO‐type proteins purified from bovine brain membranes

“…The cr subunits of Gi serve as the substrate of pertussis toxin-(lAP-) catalyzed ADP-ribosylation in their trimeric forms, have been classified into three subtypes of Oti-1, O/i-2 and o'i-3 from analyses of molecular cloning of the genes [2] and cDNAs [3][4][5][6]. In regard to the cesubunits of Go, at least two types of the genes were informed from Northern blot analysis [7].…”

Isolation and characterization of the human Gs alpha gene.

“…Recent progress in molecular cloning of Ga cDNAs revealed that they are highly similar proteins (4). So far, four Gsa (5), three Gia (4,6,41), two Gta (7), and Goa (4) cDNAs were isolated and their sequences were determined (see also ref. 1).…”