Primary structure of O- and N-glycosylic carbohydrate chains derived from murine submandibular mucin (MSM)

Abstract

The carbohydrate moiety of mouse submandibular mucin (MSM) contains mainly D-mannose and 2-acetamido-2-deoxy-o-glucose together with sialic acid, Dgalactose, and 2-acetamido-2-deoxy-D-galactose. 0-Glycosylically bound saccharides, obtained by treatment of MSM with alkaline borohydride, were shown by methylation analysis to have the structure: a-NeuAc-(243)~P-Gal-(1+3)-GalNAc-01. N-Glycosylically bound saccharides obtained from MSM by hydrazinolysis, and analysed by 500-MHz 'H-n.m.r. spectroscopy, were shown to have the following comprehensive structures.

Glycosylation Pattern and Cell Attachment‐Inhibiting Property of Human Salivary Mucins

“…Enzymic and chemical treatment as employed in this work for selective cleavage of carbohydrate residues was chosen on the basis of previous information on carbohydrate composition of this highly glycosylated and sulfated salivary constituent. 5,6,25,29,30 The results of the present study reveal an important role of carbohydrate residues in fibroblast attachmentinhibiting property of Muc. While the presence of some relatively negligible amounts of N-glycosidically linked carbohydrates in salivary mucin-type glycoproteins has been reported, 5,29 they do not appear to be involved in the inhibition of fibroblast attachment as shown by the present findings.…”

The Historical Development of Salivary Research in The Netherlands

“…At that time, the scientific community received this with skepticism, since the contemporary general dogma stated that mucins possessed only 0-glycans. Therefore, the studies on the presence of N-glycans in salivary mucins were quite difficult to publish (Nieuw Amerongen etal., 1983, 1987.…”