Primary structure of human skeletal growth factor: homology with human insulin-like growth factor-II

Mohan, Subburaman; Jennings, John C.; Linkhart, Thomas A.; Baylink, David J.

doi:10.1016/0304-4165(88)90127-4

Cited by 198 publications

(78 citation statements)

References 28 publications

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“…Previous studies have shown that human bone cells in culture produce IGF-II in vitro (19,20 To further purify the BP, peaks 1 and 2 (fractions 37-51) from the TE89 CM were pooled and subjected to Mono Q anion-exchange chromatography using a gradient of NaCl (data not shown). Again, there were two peaks of BP activity.…”

Section: Resultsmentioning

confidence: 99%

“…Specific binding was determined by subtracting nonspecific binding (in the presence of excess of unlabeled IGF-I) from total binding. IGF-I and IGF-II were radiolabeled with Na125I by a modification of the chloramine-T method as described (19). (ii) Radioreceptor assay for IGF-IL: H-35 rat hepatoma cells, which contain abundant IGF-II but not IGF-I receptors, were used as the receptor source (21).…”

Section: Methodsmentioning

confidence: 99%

“…IGF-I purified from bone matrix extract was used as standard and tracer. The assay was carried out as described (19). (iii) Cell proliferation assay: The proliferation of bone cells in serum-free culture was assayed by the incorporation of [3H]thymidine into tricholoroacetic acid-precipitable material (22).…”

Section: Methodsmentioning

confidence: 99%

“…Amino acid composition was analyzed with an Applied Biosystems model 420 instrument after hydrolysis in evacuated tubes for 24 hr at 110'C with 6 M HCI/0.5% phenol. N-terminal sequences were determined as described (19).…”

Section: Methodsmentioning

confidence: 99%

“…We have recently shown that skeletal growth factor isolated from human bones is very similar, if not identical, to IGF-II (19). The findings that human bone cell CM contains a large molecular weight form of IGF-I1 suggested that human bone cells in culture produce IGF-II and its BP (20).…”

mentioning

confidence: 99%

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Isolation of an inhibitory insulin-like growth factor (IGF) binding protein from bone cell-conditioned medium: a potential local regulator of IGF action.

Mohan

Bautista

Wergedal

et al. 1989

Proc. Natl. Acad. Sci. U.S.A.

331

127

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Inhibitory insulin-like growth factor binding protein (In-IGF-BP) has been purified to homogeneity from medium conditioned by TE89 human osteosarcoma cells by two different methods using Sephadex G-100 gel filtration, FPLC Mono Q ion-exchange, HPLC C4 reverse-phase, HPLC CN reverse-phase, and affinity chromatographies. In-IGF-BP thus purified appeared to be homogeneous and unique by the following criteria. (i) N-terminal sequence analysis yielded a unique sequence (Asp-Glu-Ala-Ile-His-Cys-Pro-Pro-GluSer-Glu-Ala-Lys-Leu-Ala). (i) Amino acid composition of In-IGF-BP revealed marked differences with the amino acid compositions of other known BPs. (iii) In-IGF-BP exhibited a single band with a molecular mass of 25 kDa under reducing conditions on sodium dodecyl sulfate/polyacrylamide gels.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

See 3 more Smart Citations

Isolation of an inhibitory insulin-like growth factor (IGF) binding protein from bone cell-conditioned medium: a potential local regulator of IGF action.

Mohan

Bautista

Wergedal

et al. 1989

Proc. Natl. Acad. Sci. U.S.A.

331

127

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Possible paracrine mechanism of insulin-like growth factor-2 in the development of liver metastases from colorectal carcinoma

Kawamoto

Onodera

Kan

et al. 1999

Cancer

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Growth factor regulation of insulin-like growth factor binding protein-6 expression in osteoblasts

Gabbitas

Canalis

1997

J. Cell. Biochem.

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Previously we have shown that transforming growth factor beta (TGF beta) 1, basic fibroblast growth factor (FGF), and platelet-derived growth factor (PDGF) BB inhibit the synthesis of insulin-like growth factor (IGF) II, but their effects on IGF binding protein (IGFBP)-6 in osteoblast cultures are not known. IGFBP-6 binds IGF II with high affinity and prevents IGF II-mediated effects, so that a possible mode of regulating the IGF II available to bone cells would be by changing the levels of IGFBP-6. To enhance our understanding of the actions of growth factors on the IGF II axis in bone, we tested the effects of TGF beta 1, basic FGF, PDGF BB, IGF I, and IGF II on the expression of IGFBP-6 in cultures of osteoblast-enriched cells from 22 day fetal rat calvariae (Ob cells). Treatment of Ob cells with TGF beta 1 caused a time- and dose-dependent decrease in IGFBP-6 mRNA levels, as determined by Northern blot analysis. The effect was maximal after 48 h and observed with TGF beta 1 concentrations of 0.04 nM and higher. TGF beta 1 also decreased IGFBP-6 polypeptide levels in the medium, as determined by Western immunoblot analysis. Cycloheximide at 3.6 microM decreased IGFBP-6 transcripts and prevented the effect of TGF beta 1. The decay of IGFBP-6 mRNA in transcriptionally arrested Ob cells was not modified by TGF beta 1. In addition, TGF beta 1 decreased the rates of IGFBP-6 transcription as determined by a nuclear run-on assay. In contrast, basic FGF, PDGF BB, IGF I, and IGF II did not change IGFBP-6 mRNA levels in Ob cells. In conclusion, TGF beta 1 inhibits IGFBP-6 expression in Ob cells by transcriptional mechanisms. Since IGFBP-6 binds IGF II and prevents its effects on bone cells, decreased synthesis of IGFBP-6 induced by TGF beta 1 could be a local feedback mechanism to increase the amount of IGF II available in the bone microenvironment.

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Primary structure of human skeletal growth factor: homology with human insulin-like growth factor-II

Cited by 198 publications

References 28 publications

Isolation of an inhibitory insulin-like growth factor (IGF) binding protein from bone cell-conditioned medium: a potential local regulator of IGF action.

Isolation of an inhibitory insulin-like growth factor (IGF) binding protein from bone cell-conditioned medium: a potential local regulator of IGF action.

Possible paracrine mechanism of insulin-like growth factor-2 in the development of liver metastases from colorectal carcinoma

Growth factor regulation of insulin-like growth factor binding protein-6 expression in osteoblasts

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