Primary structure of an environmental stress and abscisic acid-inducible alfalfa protein

Luo, Ma; Lin, Li-Yun; Hill, Robert D.; Mohapatra, Shyam S.

doi:10.1007/bf00028745

Cited by 33 publications

(22 citation statements)

References 10 publications

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“…In contrast, CAP85 does not contain a serine cluster [22]. CAP85 is not arginine-rich like the cold-regulated genes from barley [4] nor is it enriched in glycine [45]. The structure of CAP85 as determined from the deduced amino acid sequence showed a number of interesting characteristics.…”

Section: Discussionmentioning

confidence: 97%

“…A number of plant genes upregulated in response to low temperature have recently been characterized [4,21,22,40,45,54]. A cold-inducible gene from alfalfa, pSM2075 [45], encodes a protein which, like the dehydrin B18 from barley [9], is glycine-rich.…”

Section: Discussionmentioning

confidence: 99%

“…A cold-inducible gene from alfalfa, pSM2075 [45], encodes a protein which, like the dehydrin B18 from barley [9], is glycine-rich. However, the cold-inducible gene does not encode either the serine cluster nor the lysine-rich repeat which are characteristic of Group 2 LEAs.…”

Section: Discussionmentioning

confidence: 99%

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Characterization of a spinach gene responsive to low temperature and water stress

et al. 1993

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The characterization of a cDNA for an 85 kDa spinach protein, CAP85 (cold acclimation protein) that is responsive to cold acclimation and water stress is described. Both transcript and protein levels are increased during cold acclimation and water stress. A novel characteristic of CAP85 is the presence of an 11 amino acid, lysine-rich repeat, common to Group 2 LEAs (late embryogenesis abundant proteins), which is included within a larger repeating motif present in 11 copies. Two other motifs of 8 and 16 residues are also found in three and four copies, respectively. CAP85 like other dehydrins and cold-regulated polypeptides remains soluble upon boiling. Protein blot analyses indicate that CAP85 protein is expressed in all aerial tissues as well as in roots. RNA blots show the presence of mRNA for the 85 kDa protein in leaf, petiole, and root tissue. Cell fractionation studies suggest that CAP85 is predominantly found in the cytosol.

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

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Characterization of a spinach gene responsive to low temperature and water stress

et al. 1993

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“…Biochemical and molecular studies have shown that cold acclimation involves the synthesis of a specific subset of proteins resulting from differential gene expression [7,20,25,34,36]. On the other hand, it is known that drought stress can induce increased freezing tolerance in winter rye [53] and cabbage [12].…”

Section: Introductionmentioning

confidence: 99%

Induction of chilling resistance by water stress, and cDNA sequence analysis and expression of water stress-regulated genes in rice

Takahashi¹,

Joshee²,

Kitagawa³

1994

Plant Mol Biol

111

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Exposure of seedlings of a chilling-sensitive variety of rice (Oryza sativa L. cv. Wasetoittu) to water stress (0.5 M mannitol, 30 min) at room temperature induced a degree of chilling resistance. No such resistance was induced by exogenous abscisic acid (ABA) application (10 microM, 60 min). Upon short-term water stress, new transcripts were expressed in both seedlings and suspension-cultured cells. We suggest that the genes induced by short-term water stress, and not those induced by ABA, are related to acquired chilling resistance in this chilling-sensitive rice variety. A total of nine different cDNA clones, specifically induced by short-term water stress, were isolated by differential hybridization and partial sequencing. Northern hybridization analysis using RNAs from the seedlings subjected to chilling after water stress treatment reveal three distinct groups of above mentioned nine cDNA clones: wsi (water stress-induced) 18, 76, and 724, representative of genes whose expression increases, decreases, and remains almost fixed during chilling, respectively. The nucleotide and deduced amino acid sequences of the three representative clones were determined. Characteristic features of wsi18 are the presence of one set of amino acid sequence repeats, a conserved amino acid sequence common to LEA-group genes in the N-terminal region, and an alanine- and lysine-rich tract in the C-terminal region.

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“…Proline-rich proteins constitute a class of structural proteins that are known to be environmentally and developmentally regulated in plants [23]. Although many COR genes have been isolated and characterized in alfalfa [11,13,14,18,24] and in other plant species [4,5,12], m s a C I C is the first reported cold-regulated proline-rich protein.…”

mentioning

confidence: 99%

A cold-induced gene from Medicago sativa encodes a bimodular protein similar to developmentally regulated proteins

Castonguay¹,

Laberge²,

Nadeau³

et al. 1994

Plant Mol Biol

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A new cold-regulated (COR) gene, msa CIC, was isolated by differential screening of a cDNA library from cold-acclimated crowns of alfalfa (Medicago sativa L. cv. Apica). Transcripts of msa CIC were not detectable in unacclimated alfalfa and accumulated to higher levels in cold-acclimated plants of the cold-tolerant cv. Apica than in those of the cold-sensitive cv. CUF-101. The DNA sequence analysis of a full-length cDNA clone revealed that msa CIC encodes for a putative protein (MSACIC) of 166 amino acids with distinct proline-rich and hydrophobic domains. Protein sequence comparisons indicated that MSACIC is similar to a group of bimodular proteins that are developmentally regulated in other plant species.

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Primary structure of an environmental stress and abscisic acid-inducible alfalfa protein

Cited by 33 publications

References 10 publications

Characterization of a spinach gene responsive to low temperature and water stress

Characterization of a spinach gene responsive to low temperature and water stress

Induction of chilling resistance by water stress, and cDNA sequence analysis and expression of water stress-regulated genes in rice

A cold-induced gene from Medicago sativa encodes a bimodular protein similar to developmentally regulated proteins

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