Primary structure of a lipoxygenase from barley grain as deduced from its cDNA sequence

Mechelen, J.R. van; Smits, M.; Douma, A.C.; Rouster, Jacques; Cameron-Mills, Verena; Heidekamp, F.; Valk, B.E.

doi:10.1016/0005-2760(94)00231-m

Cited by 32 publications

(26 citation statements)

References 15 publications

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“…Nevertheless, the deduced amino acid sequence (data not shown) was consistent with the C terminus of a lipoxygenase, showing .v80% similarity to lipoxygenases from barley (Vanmechelen et al, 1995) and rice (Ohta et al, 1992). Lipoxygenases have been shown to be involved in plant-pathogen interactions in a number of plant species (Siedow, 1991;Farmer, 1994).…”

Section: E T P C S R T P R E S S~k I G P W G K I S G E F L D V P~p O mentioning

confidence: 82%

Benzothiadiazole, a novel class of inducers of systemic acquired resistance, activates gene expression and disease resistance in wheat.

et al. 1996

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Section: E T P C S R T P R E S S~k I G P W G K I S G E F L D V P~p O mentioning

confidence: 82%

Benzothiadiazole, a novel class of inducers of systemic acquired resistance, activates gene expression and disease resistance in wheat.

et al. 1996

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“…Two different isoforms, LOX-1 and LOX-2, have been purified and characterised [6]: LOX-1 contributes almost exclusively to the total LOX activity in quiescent grains, whereas LOX-2 activity increases rapidly during germination [7]. Three cDNA sequences encoding the LOX proteins have been isolated from barley grains, and the chromosomal locations of their corresponding genes determined: the LoxA and LoxC clones correspond to the LOX-1 and LOX-2 isoforms, respectively [8], and a third clone, known as LoxB [9], encodes a LOX isoform that has not been identified to date. The LoxA and LoxB loci are tightly linked (1 cM) and map to chromosome 4HS, whereas LoxC maps to the long arm of chromosome 5H [9].…”

Section: Introductionmentioning

confidence: 99%

Insight into durum wheat Lpx-B1: a small gene family coding for the lipoxygenase responsible for carotenoid bleaching in mature grains

et al. 2010

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BackgroundThe yellow colour of pasta products is one of the main criteria used by consumers to assess pasta quality. This character is due to the presence of carotenoid pigments in semolina. During pasta processing, oxidative degradation of carotenoid pigments occurs mainly due to lipoxygenase (LOX). In durum wheat (Triticum durum Desf.), two Lpx-1 genes have been identified on chromosome 4B, Lpx-B1.1 and Lpx-B1.2, and evidences have been reported that the deletion of Lpx-B1.1 is associated with a strong reduction in LOX activity in semolina. In the present study, we characterised the Lpx-B1 gene family identified in a durum wheat germplasm collection and related the distribution and expression of the Lpx-B1 genes and alleles to variations in LOX activity in the mature grains.ResultsIn addition to the already known Lpx-B1.1 and Lpx-B1.2 genes, a new gene was identified, Lpx-B1.3, along with three different Lpx-B1.1 alleles, Lpx-B1.1a, Lpx-B1.1b and the partially deleted Lpx-B1.1c. Screening of the germplasm collection showed that all of the genotypes have one of the three Lpx-B1.1 alleles, associated with either Lpx-B1.2 or Lpx-B1.3, thus showing that in this collection the two genes are alternatives. Therefore, based on Lpx-B1 distribution, three different haplotypes were distinguished: haplotype I, carrying Lpx-B1.3 and the Lpx-B1.1b allele; haplotype II carrying Lpx-B1.2 and the Lpx-B1.1a allele; and haplotype III carrying Lpx-B1.2 and the Lpx-B1.1c allele. Determination of Lpx-B1 transcript abundance and total LOX activity in mature grains revealed differences among these three haplotypes: haplotypes I, II and III showed high, intermediate and low levels, respectively, of functional Lpx-B1 transcripts and enzymatic activity.ConclusionsIn this germplasm collection, the Lpx-B1 gene family accounts for most of the total LOX activity in the mature grains. Information on these Lpx-B1 haplotypes provides significant improvement for prediction of LOX-1 activity levels in mature grains, and will therefore help in breeding programmes aimed at selection of new durum wheat genotypes with higher carotenoid contents in their end products.

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“…This report proved that Lox is one of the most important factors affecting the storage characteristics of bread wheat. LoxA, LoxB, and LoxC genes in the barley genome belong to a single copy of cloning, in which the gene sequence is relatively single (van Mechelen et al 1995(van Mechelen et al , 1999. 1977;Li et al 1999).…”

Section: Discussionmentioning

confidence: 99%

Molecular characterization of lipoxygenase genes on chromosome 4BS in Chinese bread wheat (Triticum aestivum L.)

Zhang¹,

Chen

Wu³

et al. 2015

Theor Appl Genet

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This study cloned two novel TaLox genes on chromosome of 4BS and developed a co-dominant marker, Lox-B23, in bread wheat that showed highly significant association with lipoxygenase activity. Lipoxygenase (Lox), a critical enzyme in the carotenoid biosynthetic pathway, significantly influences the color and processing quality of wheat-based products. Two novel Lox genes, designated TaLox-B2 and TaLox-B3, were cloned on chromosome 4BS of Chinese bread wheat. The deduced amino acid sequence showed that both TaLox-B2 and TaLox-B3 genes encoded an 861-aa protein and possessed a lipoxygenase superfamily domain at the 170-838 interval. Two different TaLox-B2 alleles, designated TaLox-B2a and TaLox-B2b, were subsequently discovered. A co-dominant marker, Lox-B23, was developed based on sequences of TaLox-B2a, TaLox-B2b, and TaLox-B3 genes to precisely distinguish these three alleles in Chinese bread cultivars. Among five allelic combinations of Lox genes at Lox-B1, Lox-B2, and Lox-B3 loci, wheat cultivars with TaLox-B1a/TaLox-B2a/TaLox-B3a combination exhibited the highest Lox activity, whereas those with TaLox-B1a/TaLox-B2b/TaLox-B3b combination significantly showed the lowest Lox activity. A RIL population was used to evaluate the influence of TaLox-B3a gene on Lox activity. Results showed that TaLox-B3a gene could significantly increase the Lox activity in bread wheat. Physical mapping indicated that both TaLox-B2 and TaLox-B3 genes were located on chromosome 4BS in bread wheat. This study provides useful information to further understand the molecular and genetic bases of Lox activity in bread wheat.

show abstract

Primary structure of a lipoxygenase from barley grain as deduced from its cDNA sequence

Cited by 32 publications

References 15 publications

Benzothiadiazole, a novel class of inducers of systemic acquired resistance, activates gene expression and disease resistance in wheat.

Benzothiadiazole, a novel class of inducers of systemic acquired resistance, activates gene expression and disease resistance in wheat.

Insight into durum wheat Lpx-B1: a small gene family coding for the lipoxygenase responsible for carotenoid bleaching in mature grains

Molecular characterization of lipoxygenase genes on chromosome 4BS in Chinese bread wheat (Triticum aestivum L.)

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