Primary Structure and Expression of a 24-kD Vacuolar Protein (VP24) Precursor in Anthocyanin-Producing Cells of Sweet Potato in Suspension Culture

Xu, Wenxin; Shioiri, Hidenari; Kojima, Mineo; Nozue, Masayuki

doi:10.1104/pp.125.1.447

Cited by 27 publications

(19 citation statements)

References 42 publications

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“…It is proposed that glutathione-S-transferase-like proteins such as maize BZ2 (Marrs et al, 1995), petunia AN9 (Mueller et al, 2000), and Arabidopsis TT19 (Kitamura et al, 2004) deliver their flavonoid substrates to the transporter, such as the maize multidrug resistance-associated protein (MRP)-type transporter ZmMRP3 (Goodman et al, 2004) or the Arabidopsis multidrug and toxic compound extrusion transporter TT12 . In sweet potato, it has been suggested that VP24 precursor protein is involved in vacuolar transport and/or accumulation of anthocyanin (Nozue et al, 1997;Xu et al, 2001).…”

Section: Discussionmentioning

confidence: 99%

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Isolation of a Regulatory Gene of Anthocyanin Biosynthesis in Tuberous Roots of Purple-Fleshed Sweet Potato

Mano¹,

Ogasawara²,

Sato³

et al. 2007

Plant Physiol.

246

185

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Many transcriptional factors harboring the R2R3-MYB domain, basic helix-loop-helix domain, or WD40 repeats have been identified in various plant species as regulators of flavonoid biosynthesis in flowers, seeds, and fruits. However, the regulatory elements of flavonoid biosynthesis in underground organs have not yet been elucidated. We isolated the novel MYB genes IbMYB1 and IbMYB2s from purple-fleshed sweet potato (Ipomoea batatas L. Lam. cv Ayamurasaki). IbMYB1 was predominantly expressed in the purple flesh of tuberous roots but was not detected (or only scarcely) in other anthocyanin-containing tissues such as nontuberous roots, stems, leaves, or flowers. IbMYB1 was also expressed in the tuberous roots of other purple-fleshed cultivars but not in those of orange-, yellow-, or white-fleshed cultivars. Although the orange-or yellow-fleshed cultivars contained anthocyanins in the skins of their tuberous roots, we could not detect IbMYB1 transcripts in these tissues. These results suggest that IbMYB1 controls anthocyanin biosynthesis specifically in the flesh of tuberous roots. The results of transient and stable transformation experiments indicated that expression of IbMYB1 alone was sufficient for induction of all structural anthocyanin genes and anthocyanin accumulation in the flesh of tuberous roots, as well as in heterologous tissues or heterologous plant species.

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Section: Discussionmentioning

confidence: 99%

“…Genes harboring KPRPR(S/T) F-like motif correspond to the genes of the N09 subgroup in Figure 4C. transport and/or trapping (Nozue et al, 1997;Xu et al, 2001).…”

Section: Tissue-specific Expression Of Genes Involved In Anthocyanin mentioning

confidence: 99%

Isolation of a Regulatory Gene of Anthocyanin Biosynthesis in Tuberous Roots of Purple-Fleshed Sweet Potato

Mano¹,

Ogasawara²,

Sato³

et al. 2007

Plant Physiol.

246

185

View full text Add to dashboard Cite

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“…In sweet potato (Ipomea batatas), carnation (Dianthus caryophyllus), and lisianthus (Eustoma grandiorum), AVIs appear to lack surrounding membranes and instead consist of a protein matrix or thread-like structures (Small and Pecket, 1982;Nozue et al, 1993;Markham et al, 2000;Zhang et al, 2006;Conn et al, 2010). Besides anthocyanins, AVIs have been reported to contain a metalloprotease called VP24 in sweet potato (Nozue et al, 1997(Nozue et al, , 2003Xu et al, 2001) and tonoplast membrane lipids in grapevine cultured cells (Conn et al, 2010). In grapevine cell cultures, AVIs are enriched in acylated anthocyanins (Conn et al, 2003), suggesting that AVIs may sequester specific anthocyanin species.…”

Section: Introductionmentioning

confidence: 99%

Anthocyanin Vacuolar Inclusions Form by a Microautophagy Mechanism

et al. 2015

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Anthocyanins are flavonoid pigments synthesized in the cytoplasm and stored inside vacuoles. Many plant species accumulate densely packed, 3-to 10-mm diameter anthocyanin deposits called anthocyanin vacuolar inclusions (AVIs). Despite their conspicuousness and importance in organ coloration, the origin and nature of AVIs have remained controversial for decades. We analyzed AVI formation in cotyledons of different Arabidopsis thaliana genotypes grown under anthocyanin inductive conditions and in purple petals of lisianthus (Eustoma grandiorum). We found that cytoplasmic anthocyanin aggregates in close contact with the vacuolar surface are directly engulfed by the vacuolar membrane in a process reminiscent of microautophagy. The engulfed anthocyanin aggregates are surrounded by a single membrane derived from the tonoplast and eventually become free in the vacuolar lumen like an autophagic body. Neither endosomal/prevacuolar trafficking nor the autophagy ATG5 protein is involved in the formation of AVIs. In Arabidopsis, formation of AVIs is promoted by both an increase in cyanidin 3-O-glucoside derivatives and by depletion of the glutathione S-transferase TT19. We hypothesize that this novel microautophagy mechanism also mediates the transport of other flavonoid aggregates into the vacuole.

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“…The structure has been shown to be, in part, comprised of a hydrophobic proteinaceous matrix which colocalises with anthocyanins. A component of the AVIs was determined to be a 24-kDa protein (VP24), which has been purified and localised to the AVIs by immuno-localisation (Nozue et al , 1997 and the gene cloned (Xu et al 2001).…”

Section: Introductionmentioning

confidence: 99%

Characterization of anthocyanic vacuolar inclusions in Vitis vinifera L. cell suspension cultures

Conn

Franco

Zhang

2010

Planta

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Anthocyanic vacuolar inclusions (AVIs) are intra-vacuolar structures capable of concentrating anthocyanins and are present in over 50 of the highest anthocyanin-accumulating plant species. Presence of AVIs alters pigment intensity, total anthocyanin levels, pigment hue and causes bathochromic shifts in a spatio-temporal manner within various flowers, vegetables and fruits. A year-long study on Vitis vinifera cell suspension cultures found a strong correlation between AVI prevalence and anthocyanin content, but not the number of pigmented cells, growth rate or stilbene content. Furthermore, enhancement of the prevalence of AVIs and anthocyanins was achieved by treatment of V. vinifera cell suspension cultures with sucrose, jasmonic acid and white light. A unique autofluorescence of anthocyanins was used to demonstrate microscopically that AVIs proceed from the cytosol across the tonoplast and were able to coalesce intravacuolarly, with fewer, larger AVIs predominating as cells mature. Purification and characterisation of these bodies were performed, showing that they were dense, highly organic structures, with a lipid component indicative of membrane-encasement. These purified AVIs were also shown to comprise long-chain tannins and possessed an increased affinity for binding acylated anthocyanins, though no unique protein component was detected.

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Primary Structure and Expression of a 24-kD Vacuolar Protein (VP24) Precursor in Anthocyanin-Producing Cells of Sweet Potato in Suspension Culture

Cited by 27 publications

References 42 publications

Isolation of a Regulatory Gene of Anthocyanin Biosynthesis in Tuberous Roots of Purple-Fleshed Sweet Potato

Isolation of a Regulatory Gene of Anthocyanin Biosynthesis in Tuberous Roots of Purple-Fleshed Sweet Potato

Anthocyanin Vacuolar Inclusions Form by a Microautophagy Mechanism

Characterization of anthocyanic vacuolar inclusions in Vitis vinifera L. cell suspension cultures

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