Primary structure analysis of the major internal protein p24 of human type C T-cell leukemia virus

Abstract

Primary structure analysis of the major internal protein p24 of human type C T-cell leukemia virus ( Communicated by Lloyd J. Old, November 16, 1981 ABSTRACT A human type C retrovirus [human T-cell leukemia (lymphoma) virus; HTLV], recently isolated from young adult patients with cutaneous T-cell lymphoma or leukemia, was not detectably related to other known animal retroviruses in molecular hybridization studies, by comparison of reverse transcriptase and the major core protein p24. The p24 core protein was purified to homogeneity. The amino acid composition, the COOHterminal amino acid, and the NH2-terminal amino acid sequence of the first 25 residues of this major internal structural protein were determined. These results were then compared to the known structure of the internal core protein of other retroviruses. The compositional data reveal that HTLV p24 is chemically distinct from p3O-p24 ofother animal retroviruses, in agreement with the earlier immunological analyses. However, HTLV p24 shares the common NH2-terminal proline and COOH-terminal leucine of all mammalian type C viral p3Os. In addition, like bovine leukemia virus (BLV), HTLV lacks the common prolylleucylarginine tripeptide and the larger conserved region found near the

Complete amino acid sequence of human T‐cell leukemia virus structural protein p15

“…Extensive biochemical and immunological studies provided evidence that HTLV is a unique retrovirus dinstinct from all previously known isolates [2,5]. Amino acid sequence analysis of the major internal protein p24 [6] confirmed these findings and revealed for the first time a structural relationship between HTLV p24 and protein homologs in other retroviruses, especially bovine leukemia virus (BLV). The initial NH2-terminal analysis and the results of ongoing studies show an -37% homology of the major core pro-…”

Bacterial expression of the acquired immunodeficiency syndrome retrovirus p24 gag protein and its use as a diagnostic reagent.

“…1 shows the sequence, derived from both the protein and DNA sequence (14), for the first 25 amino acids of the AIDS retrovirus p24 gag protein. This region of the AIDS retrovirus gag sequence shows few amino acids in common with either the two other known human retrovirus core proteins (24,25) or the core proteins from two other animal retroviruses (26,27), although the homology to the bovine leukemia virus p24 NH2 terminus (27) (16). The sequence is shown compared with two other human retroviruses, HTLV-I (24) and HTLV-II (25), and two animal retroviruses, bovine leukemia virus (BLV) (26) and feline leukemia virus (FeLV) (27).…”

Viral Aetiology of Adult T-Cell Leukaemia

“…This polypeptide is a major component of the virion. Determination of the amino acid sequence of the HTLV p24 revealed a statistically significant sequence homology with bovine leukaemia virus (BLV) (Oroszlan et al, 1982). Earlier, Kalyanaraman et al had detected precipitation of p24 and pl9 when iodine-labelled HTLV was reacted with sera from patients CR and NB, from whom HTLV had been derived, and the wife of CR Posner et al, 1981).…”