Primary Stability of a PGLA/Polydioxanone Membrane for Potential Autologous Chondrocyte Implantation in the Hip Joint. a Cadaveric Study

Abstract

The purpose of this cadaveric study was to evaluate the primary stability of a membrane (resorbable polyglactin-910/poly-p-dioxanone) for autologous chondrocyte implantation (ACI) inserted by press-fit into defects in the femoral head and acetabulum. The stability of the membrane was evaluated after implantation in a cartilage defect on both sides of the joint in 12 hips in six cadavers. The hip was manually put through a full range of motion for 50 cycles after each lesion had been created and filled, starting with the acetabulum. The implanted membranes showed stability in 83.3% of the acetabular defects and in 33.3% of the femoral defects after the 50 cycles. After an additional 50 cycles the acetabular membrane showed stability in a total of eight (67%) out of 12 acetabular lesions. The results indicate that this membrane tissue is potentially stable in cartilage defects of the acetabulum, which may have clinical applications in autologous chondrocyte implantation.

Characterization of Chondrogenic Gene Expression and Cartilage Phenotype Differentiation in Human Breast Adipose-Derived Stem Cells Promoted by Ginsenoside Rg1 In Vitro

“…Remove the fixation solution, and wash cells with 1× PBS (pH adjusted to 4.2) 1-2 times (3). Add a filtered 2% Alizarin Red solution to the fixed cells and incubate them at 37°C for 10-20 minutes (Note: monitor staining under a microscope every 2-5 min) (4). Remove the Alizarin Red solution, and rinse cells with PBS 1-2 times (5).…”

“…Place in absolute propylene glycol for 5 minutes to avoid transferring water into the Oil Red O. (3) Stain in Oil Red O solution for 8 minutes in 60°C oven (4). Rinse in 85% propylene glycol solution for 5 minutes (5).…”

“…Stain in Alcian blue solution for 30 minutes (3). Wash in running tap water for 2 minutes, and rinse in distilled water (4). Counterstain in nuclear Fast Red solution for 5 minutes, and wash in running tap water for 1 minute (5).…”

“…This technique consists of three main steps, including the isolation of chondrocytes from healthy cartilage tissue, chondrocyte cultivation or expansion in vitro over 2-3 weeks, and re-injection of chondrocytes into the injured cartilage, covered with a periosteal flap [1]. This method gained international acceptance within the orthopedic surgery field [2,3] and was further refined by adding biomaterials such as coated scaffolds, membranes, and different matrices [4,5]. Despite this wide acceptance, several studies have revealed certain problems and limitations of ACT, including cell leakage, the requirement for high cell concentrations, and apoptosis of the re-injected chondrocytes.…”

Comparison of four chondral repair techniques in the hip joint: a biomechanical study using a physiological human cadaveric model

“…The closest approximation to the natural environment is offered by the human cadaveric hip, which could be used to simulate the gait cycle in the post-operative rehabilitation period 10,12 . Previous studies testing cartilage repair in a cadaveric model have used simple sinus cyclic loading in both the knee and hip joint 19,21,36 . Fontana et al have recently reported on the stability of polydioxanone scaffolds in a cadaveric hip joint using continues passive motion performed by a manual operator 36 .…”