Primary screening of potentially bio-active substances in the lyophilisate of Pectinatella magnifica biomass

Abstract

ABSTRACT:The purpose of this research was to examine potential bio-active substances in the lyophilisate of Pectinatella magnifica biomass and its focus was on primary screening of the biomass. Extracts of lyophilisate were applied to murine cells and their effects on cell population growth, cell morphology and cell distribution were monitored. The inhibition of cytoskeleton repair reached its maximum after a 24-h exposure of the cells to the extract prepared at a temperature of 37 °C, whereas the 1-h application of the extract leached at a temperature of 37 °C resulted in quick repair of cytoskeletal function. When compared with the control, in which no extract was applied, the application of all treatments of the extract reduced the time needed for the duplication of cells. The lowest growth rate was found in the cells in the extract prepared at 37 °C for 24 h. The mitotic activity reached its maximum when using the extract prepared at 37 °C for 1 h. Under such conditions, the process of cell duplication was even faster than in the control sample, while it was the slowest using the extract prepared at 37 °C for 24 h. The study of potential biologically active substances of Pectinatella magnifica is highly topical due to the frequent occurrence of this organism in the water-supply and recreational reservoirs.

AFLP reveals low genetic diversity of the bryozoan Pectinatella magnifica (Leidy, 1851) in the Czech Republic

“…Once formed, colonies exude a protective gelatin-like matrix of exopolymers, enabling attachment to different submerged natural or artificial substrates [ 6 ] as flat sheets (in the form of thin films), as sessile globular colonies, or as gelatinous balls floating in water. The colonies are covered on their surface with rosettes, each containing 12–18 genetically identical zooids and can lead to considerable dimension and weight, as in the case of nearly 1 m diameter and 70 kg colony described by Balounová et al [ 7 ]. Recent studies have focused on the determination of evolutionary relationships within P. magnifica , incorporating morphological traits (such as the morphology of the statoblasts, white spots on lophophora, pigmented mouth), molecular approaches, or a combination of both morphological traits and genetic data [ 8 ].…”

“…This fact clearly demonstrates that a high amount of genetic diversity is not necessarily a condition for global spread over contemporary timescales. Given the fact that P. magnifica was first reported in Europe in 1883 [ 7 ], a low diversity across Europe is expected, although it could also be possible that P. magnifica was previously undetected. The results of genetic analyses performed in this study on P. magnifica colonies sampled within 5 years across different localities correspond with the so called genetic paradox in invasive species, where invasive populations are able to overcome low genetic diversity and inbreeding to thrive in the invaded environment.…”